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A9CFX5

- BIOB_AGRT5

UniProt

A9CFX5 - BIOB_AGRT5

Protein

Biotin synthase

Gene

bioB

Organism
Agrobacterium tumefaciens (strain C58 / ATCC 33970)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi75 – 751Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi112 – 1121Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi143 – 1431Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi203 – 2031Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi275 – 2751Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciAGRO:ATU3997-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Atu3997
    ORF Names:AGR_L_1708
    OrganismiAgrobacterium tumefaciens (strain C58 / ATCC 33970)
    Taxonomic identifieri176299 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex
    ProteomesiUP000000813: Chromosome linear

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 339339Biotin synthasePRO_0000381187Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi176299.Atu3997.

    Structurei

    3D structure databases

    ProteinModelPortaliA9CFX5.
    SMRiA9CFX5. Positions 23-329.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiDETQALC.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9CFX5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQLATQIDG KPASIPAVET SSSLEEAKII YNLPFNDLLF RAQQVHRCHF    50
    DANAIQMSRL LSIKTGGCPE DCSYCSQSAR NPTGLKASKL MEVERVLAEA 100
    RKAKEGGATR YCMGAAWRNP KERDMEAVVA MVEGVKALDM ETCMTLGMLT 150
    PEQSERLADA GLDYYNHNVD TSERFYSEII TTRTFEDRLE TLANVRDAGI 200
    KVCAGGILGM GETVEDRISM LVTLANLPVP PESVPINMLI PIPGSKLANA 250
    DPVDPIDFVR TIALARILMP RSHVRLSAGR TEMSDETQAL CFLAGANSIF 300
    IGETLLTADN PGEDHDTALF RRLGLKPMEL QSSEAGGCR 339
    Length:339
    Mass (Da):37,013
    Last modified:January 15, 2008 - v1
    Checksum:i589ACD7924AC263B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007870 Genomic DNA. Translation: AAK89428.1.
    PIRiAI3047.
    B98238.
    RefSeqiNP_356643.1. NC_003063.2.

    Genome annotation databases

    EnsemblBacteriaiAAK89428; AAK89428; Atu3997.
    GeneIDi1135871.
    KEGGiatu:Atu3997.
    PATRICi20817426. VBIAgrTum91616_3816.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE007870 Genomic DNA. Translation: AAK89428.1 .
    PIRi AI3047.
    B98238.
    RefSeqi NP_356643.1. NC_003063.2.

    3D structure databases

    ProteinModelPortali A9CFX5.
    SMRi A9CFX5. Positions 23-329.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 176299.Atu3997.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAK89428 ; AAK89428 ; Atu3997 .
    GeneIDi 1135871.
    KEGGi atu:Atu3997.
    PATRICi 20817426. VBIAgrTum91616_3816.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi DETQALC.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci AGRO:ATU3997-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C58 / ATCC 33970.

    Entry informationi

    Entry nameiBIOB_AGRT5
    AccessioniPrimary (citable) accession number: A9CFX5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3