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A9CFX5 (BIOB_AGRT5) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Atu3997
ORF Names:AGR_L_1708
OrganismAgrobacterium tumefaciens (strain C58 / ATCC 33970) [Reference proteome] [HAMAP]
Taxonomic identifier176299 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeRhizobium/Agrobacterium groupAgrobacteriumAgrobacterium tumefaciens complex

Protein attributes

Sequence length339 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 339339Biotin synthase HAMAP-Rule MF_01694
PRO_0000381187

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2031Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2751Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9CFX5 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 589ACD7924AC263B

FASTA33937,013
        10         20         30         40         50         60 
MDQLATQIDG KPASIPAVET SSSLEEAKII YNLPFNDLLF RAQQVHRCHF DANAIQMSRL 

        70         80         90        100        110        120 
LSIKTGGCPE DCSYCSQSAR NPTGLKASKL MEVERVLAEA RKAKEGGATR YCMGAAWRNP 

       130        140        150        160        170        180 
KERDMEAVVA MVEGVKALDM ETCMTLGMLT PEQSERLADA GLDYYNHNVD TSERFYSEII 

       190        200        210        220        230        240 
TTRTFEDRLE TLANVRDAGI KVCAGGILGM GETVEDRISM LVTLANLPVP PESVPINMLI 

       250        260        270        280        290        300 
PIPGSKLANA DPVDPIDFVR TIALARILMP RSHVRLSAGR TEMSDETQAL CFLAGANSIF 

       310        320        330 
IGETLLTADN PGEDHDTALF RRLGLKPMEL QSSEAGGCR 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE007870 Genomic DNA. Translation: AAK89428.1.
PIRAI3047.
B98238.
RefSeqNP_356643.1. NC_003063.2.

3D structure databases

ProteinModelPortalA9CFX5.
SMRA9CFX5. Positions 23-329.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING176299.Atu3997.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAK89428; AAK89428; Atu3997.
GeneID1135871.
KEGGatu:Atu3997.
PATRIC20817426. VBIAgrTum91616_3816.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMADETQALC.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycAGRO:ATU3997-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_AGRT5
AccessionPrimary (citable) accession number: A9CFX5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways