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A9C2R2 (BIOB_DELAS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Daci_3779
OrganismDelftia acidovorans (strain DSM 14801 / SPH-1) [Complete proteome] [HAMAP]
Taxonomic identifier398578 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 353353Biotin synthase HAMAP-Rule MF_01694
PRO_0000381343

Sites

Metal binding871Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding911Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1621Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2221Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2941Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9C2R2 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 62DBF333CF284EF5

FASTA35337,780
        10         20         30         40         50         60 
MTACSTTPTT SATSAQPAAG SPLQWHARPS KSDTLPFEGA WSVQAIQELL DKPFMDLMFQ 

        70         80         90        100        110        120 
AQSVHREHWP AGDIELATLL SVKTGGCPEN CGYCPQAAEF DTGVKAEKLM SVDEVTRAAQ 

       130        140        150        160        170        180 
AAKDAGATRF CMGAAWRAPK DRDIEKMNEL IGAVKGLGMQ TCATLGMLQP HQAQSLREAG 

       190        200        210        220        230        240 
LDYYNHNLDT APEYYQDVVS TRQYQDRLDT LKAVRGAGIS VCCGGIIGMG EAPVHRAGLI 

       250        260        270        280        290        300 
AQLANLNPHP ESVPINSLVR VPGTPLADSE PVDPLDFVRV IAVARITMPT ARVRLSAGRQ 

       310        320        330        340        350 
QLGDAVQALC FMAGANSIFY GDKLLVTGNP DVEADTTLLR KLGLSGTRTS VQE 

« Hide

References

[1]"Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14801 / SPH-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000884 Genomic DNA. Translation: ABX36411.1.
RefSeqYP_001564796.1. NC_010002.1.

3D structure databases

ProteinModelPortalA9C2R2.
SMRA9C2R2. Positions 40-348.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398578.Daci_3779.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX36411; ABX36411; Daci_3779.
GeneID5749362.
KEGGdac:Daci_3779.
PATRIC21641501. VBIDelAci41351_3808.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239957.
KOK01012.
OMARIMMPAS.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycDACI398578:GHK3-3826-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_DELAS
AccessionPrimary (citable) accession number: A9C2R2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways