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A9C2R2

- BIOB_DELAS

UniProt

A9C2R2 - BIOB_DELAS

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Protein
Biotin synthase
Gene
bioB, Daci_3779
Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi162 – 1621Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi222 – 2221Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi294 – 2941Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciDACI398578:GHK3-3826-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Daci_3779
OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
Taxonomic identifieri398578 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
ProteomesiUP000000784: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 353353Biotin synthaseUniRule annotation
PRO_0000381343Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi398578.Daci_3779.

Structurei

3D structure databases

ProteinModelPortaliA9C2R2.
SMRiA9C2R2. Positions 40-348.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239957.
KOiK01012.
OMAiRIMMPAS.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

A9C2R2-1 [UniParc]FASTAAdd to Basket

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MTACSTTPTT SATSAQPAAG SPLQWHARPS KSDTLPFEGA WSVQAIQELL    50
DKPFMDLMFQ AQSVHREHWP AGDIELATLL SVKTGGCPEN CGYCPQAAEF 100
DTGVKAEKLM SVDEVTRAAQ AAKDAGATRF CMGAAWRAPK DRDIEKMNEL 150
IGAVKGLGMQ TCATLGMLQP HQAQSLREAG LDYYNHNLDT APEYYQDVVS 200
TRQYQDRLDT LKAVRGAGIS VCCGGIIGMG EAPVHRAGLI AQLANLNPHP 250
ESVPINSLVR VPGTPLADSE PVDPLDFVRV IAVARITMPT ARVRLSAGRQ 300
QLGDAVQALC FMAGANSIFY GDKLLVTGNP DVEADTTLLR KLGLSGTRTS 350
VQE 353
Length:353
Mass (Da):37,780
Last modified:January 15, 2008 - v1
Checksum:i62DBF333CF284EF5
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000884 Genomic DNA. Translation: ABX36411.1.
RefSeqiYP_001564796.1. NC_010002.1.

Genome annotation databases

EnsemblBacteriaiABX36411; ABX36411; Daci_3779.
GeneIDi5749362.
KEGGidac:Daci_3779.
PATRICi21641501. VBIDelAci41351_3808.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000884 Genomic DNA. Translation: ABX36411.1 .
RefSeqi YP_001564796.1. NC_010002.1.

3D structure databases

ProteinModelPortali A9C2R2.
SMRi A9C2R2. Positions 40-348.
ModBasei Search...

Protein-protein interaction databases

STRINGi 398578.Daci_3779.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX36411 ; ABX36411 ; Daci_3779 .
GeneIDi 5749362.
KEGGi dac:Daci_3779.
PATRICi 21641501. VBIDelAci41351_3808.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239957.
KOi K01012.
OMAi RIMMPAS.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci DACI398578:GHK3-3826-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14801 / SPH-1.

Entry informationi

Entry nameiBIOB_DELAS
AccessioniPrimary (citable) accession number: A9C2R2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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