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A9C2R2

- BIOB_DELAS

UniProt

A9C2R2 - BIOB_DELAS

Protein

Biotin synthase

Gene

bioB

Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi87 – 871Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi91 – 911Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi94 – 941Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi131 – 1311Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi162 – 1621Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi222 – 2221Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi294 – 2941Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciDACI398578:GHK3-3826-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:Daci_3779
    OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
    Taxonomic identifieri398578 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
    ProteomesiUP000000784: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 353353Biotin synthasePRO_0000381343Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi398578.Daci_3779.

    Structurei

    3D structure databases

    ProteinModelPortaliA9C2R2.
    SMRiA9C2R2. Positions 40-348.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239957.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9C2R2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTACSTTPTT SATSAQPAAG SPLQWHARPS KSDTLPFEGA WSVQAIQELL    50
    DKPFMDLMFQ AQSVHREHWP AGDIELATLL SVKTGGCPEN CGYCPQAAEF 100
    DTGVKAEKLM SVDEVTRAAQ AAKDAGATRF CMGAAWRAPK DRDIEKMNEL 150
    IGAVKGLGMQ TCATLGMLQP HQAQSLREAG LDYYNHNLDT APEYYQDVVS 200
    TRQYQDRLDT LKAVRGAGIS VCCGGIIGMG EAPVHRAGLI AQLANLNPHP 250
    ESVPINSLVR VPGTPLADSE PVDPLDFVRV IAVARITMPT ARVRLSAGRQ 300
    QLGDAVQALC FMAGANSIFY GDKLLVTGNP DVEADTTLLR KLGLSGTRTS 350
    VQE 353
    Length:353
    Mass (Da):37,780
    Last modified:January 15, 2008 - v1
    Checksum:i62DBF333CF284EF5
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000884 Genomic DNA. Translation: ABX36411.1.
    RefSeqiYP_001564796.1. NC_010002.1.

    Genome annotation databases

    EnsemblBacteriaiABX36411; ABX36411; Daci_3779.
    GeneIDi5749362.
    KEGGidac:Daci_3779.
    PATRICi21641501. VBIDelAci41351_3808.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000884 Genomic DNA. Translation: ABX36411.1 .
    RefSeqi YP_001564796.1. NC_010002.1.

    3D structure databases

    ProteinModelPortali A9C2R2.
    SMRi A9C2R2. Positions 40-348.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 398578.Daci_3779.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX36411 ; ABX36411 ; Daci_3779 .
    GeneIDi 5749362.
    KEGGi dac:Daci_3779.
    PATRICi 21641501. VBIDelAci41351_3808.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239957.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci DACI398578:GHK3-3826-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14801 / SPH-1.

    Entry informationi

    Entry nameiBIOB_DELAS
    AccessioniPrimary (citable) accession number: A9C2R2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3