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A9C1G8 (SYL_DELAS) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Daci_6003
OrganismDelftia acidovorans (strain DSM 14801 / SPH-1) [Complete proteome] [HAMAP]
Taxonomic identifier398578 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia

Protein attributes

Sequence length911 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 911911Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091312

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif659 – 6635"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6621ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9C1G8 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: BBEBCBD4F02DA4FD

FASTA911101,345
        10         20         30         40         50         60 
MQEKYNHIDV ERAAQDHWSA RDAYRVTEDQ AKPKFYACSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
MLTRQLRMKG YNVLMPMGWD AFGLPAENAA LKNKVPPAQW TYDNIAYMKK QMQAMGLAID 

       130        140        150        160        170        180 
WSREIATCDP DYYKWNQWLF LKMLEKGIAY RKTQVVNWDP VDQTVLANEQ VIDGRGWRTG 

       190        200        210        220        230        240 
APVEKREIPG YYLKITDYAQ ELLDHVQVGG DKATLTGWPE KVRLMQENWI GKSSGVRFAF 

       250        260        270        280        290        300 
THDIRGADGQ PIQDGRLYVF TTRADTIMGV TFCAVAPEHP LALHAAATSP KLAAFIEECK 

       310        320        330        340        350        360 
KGGTTEAELA VKEKEGLPTG LFVTHPLTGA QVEVWVGNYV LMSYGDGAVM GVPAHDERDF 

       370        380        390        400        410        420 
AFAKKYGIAI RQVIAVEGET FSTEAWADWY GDKQRAVCIE SGELDGLPHA AAVDKVAELL 

       430        440        450        460        470        480 
AAKGLGEKKT TWRLRDWGVS RQRYWGTPIP IIHCEDCGAQ PVPAKDLPVV LPQDLVPDGS 

       490        500        510        520        530        540 
GNPLIKSEAF HAGVVCPCCG KSARRETDTM DTFVDSSWYF MRYCDARNSE QMVAEGAEYW 

       550        560        570        580        590        600 
MRDQNAATGG SGMDQYIGGI EHAILHLLYA RFWTKVMRDL GLVKVDEPFT KLLTQGMVLN 

       610        620        630        640        650        660 
HIYSRRTAKG AKEYFWPKDV EHVFDETGKI VGAKLKAEVD SADGLLPVGT DIDYEGVGTM 

       670        680        690        700        710        720 
SKSKNNGIDP QELIEKYGAD TARLYTMFTA PPELTLEWND AAVEGSYRFL RRVYNFGAKL 

       730        740        750        760        770        780 
SQMDMAGAVQ SVAGAKSLDD VEFGKAAKSL RLEIHTVLKQ VEYDYQRMQY NTVVSGAMKM 

       790        800        810        820        830        840 
INALEDFKSF DDAGAQVALI EGFGILLRVL YPATPHLAHA LWSELGYAAH LGDVLDAPWP 

       850        860        870        880        890        900 
QVDPQALVQD EISLVLQVNG KLRGAILVSS TADKAEIERI ALANEECQKF TNGAVPKKVI 

       910 
VVPGRLVNVV V 

« Hide

References

[1]"Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14801 / SPH-1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000884 Genomic DNA. Translation: ABX38631.1.
RefSeqYP_001567016.1. NC_010002.1.

3D structure databases

ProteinModelPortalA9C1G8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING398578.Daci_6003.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX38631; ABX38631; Daci_6003.
GeneID5751634.
KEGGdac:Daci_6003.
PATRIC21646121. VBIDelAci41351_6075.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDACI398578:GHK3-6097-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DELAS
AccessionPrimary (citable) accession number: A9C1G8
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries