A9BVX4 (A9BVX4_DELAS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase HAMAP MF_00108 RuleBase RU003490
EC=2.7.7.60 HAMAP MF_00108 RuleBase RU003490

Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthase HAMAP MF_00108
MEP cytidylyltransferase HAMAP MF_00108

Gene names

Name:	ispD HAMAP MF_00108
Ordered Locus Names:	Daci_2849

Organism

Delftia acidovorans (strain DSM 14801 / SPH-1) [Complete proteome] [HAMAP]

Taxonomic identifier

398578 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Delftia

Protein attributes

Sequence length	298 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) By similarity. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Catalytic activity	CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00108 RuleBase RU003490 SAAS SAAS018294
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6. HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004138
Sequence similarities	Belongs to the IspD family. HAMAP MF_00108 RuleBase RU004393

Ontologies

Keywords
Biological process	Isoprene biosynthesis HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393
Molecular function	Nucleotidyltransferase HAMAP MF_00108 SAAS SAAS018294 RuleBase RU004393 EMBL ABX35487.1 Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Site	52	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	65	1	Transition state stabilizer By similarity HAMAP MF_00108
	Site	204	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108
	Site	257	1	Positions MEP for the nucleophilic attack By similarity HAMAP MF_00108

Sequences

	Sequence		Length	Mass (Da)
	A9BVX4 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 19D3BB20CFF94999	FASTA	298	31,084
	10 20 30 40 50 60 MAAMQACPHV HGGAWPSLQC ATMTDLLAAA APASLEPARL WALIPCAGTG SRAIAPDAPA 70 80 90 100 110 120 PELPKQYHLV AGAPMVAHTL AAFAGTARVH HTLVVIAPGD DFWRQGLPGP SPTRYSTAAC 130 140 150 160 170 180 GGATRAESVT NGLKELLARG ASAHDWVLVH DAARCLVTPE LIDALIDACE HDAVGGLLAH 190 200 210 220 230 240 KLPDTLKTAV TGPGGVRVAG TVDRSDKWLA QTPQMFRIAP LLDALEHAGS AVTDEASAME 250 260 270 280 290 AMGQRPRLVP GGAQNFKVTY PDDFALAAAV LSQRAHGATL ERYRGKRDQP QASGNPFF

References

[1]

"Complete sequence of Delftia acidovorans DSM 14801 / SPH-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000884 Genomic DNA. Translation: ABX35487.1.
RefSeq	YP_001563872.1. NC_010002.1.
3D structure databases
ProteinModelPortal	A9BVX4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9BVX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5748425.
GenomeReviews	Gene locus Daci_2849 in contig CP000884_GR.
KEGG	dac:Daci_2849.
PATRIC	21639635. VBIDelAci41351_2884.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672839.
OMA	FPQGAIL.
ProtClustDB	CLSK963069.
Family and domain databases
HAMAP	MF_00108. IspD. [Tree]
InterPro	IPR001228. ISPD_synthase. IPR018294. ISPD_synthase_CS. [Graphical view]
KO	K00991.
Pfam	PF01128. IspD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00453. IspD. 1 hit.
PROSITE	PS01295. ISPD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9BVX4_DELAS

Accession

Primary (citable) accession number: A9BVX4

Entry history

Integrated into UniProtKB/TrEMBL:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	December 14, 2011
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)