Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A9BRF0

- MASZ_DELAS

UniProt

A9BRF0 - MASZ_DELAS

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Malate synthase G

Gene

glcB

Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi434 – 4341MagnesiumUniRule annotation
Binding sitei434 – 4341GlyoxylateUniRule annotation
Metal bindingi462 – 4621MagnesiumUniRule annotation
Binding sitei543 – 5431Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei638 – 6381Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciDACI398578:GHK3-682-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Daci_0665
OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
Taxonomic identifieri398578 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
ProteomesiUP000000784: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Malate synthase GPRO_1000130889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi398578.Daci_0665.

Structurei

3D structure databases

ProteinModelPortaliA9BRF0.
SMRiA9BRF0. Positions 10-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni459 – 4624Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A9BRF0-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDRTQAHSL QVATELHRFI NEQVLPGTGV APEAFWKGFD AIVNDLAPRN
60 70 80 90 100
AALLAERDRL QTELDTWHKA HPGPITDMAG YQQFLTQIGY LVEQPKDAKA
110 120 130 140 150
TTTNVDAELA IQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIGE
160 170 180 190 200
DGGAEKGKGY NPVRGAKVIA FARDFLDQAA PLASGSHKDA AGYRVEAGQL
210 220 230 240 250
VVALKSGGTT GLKNTAQFVG YQGDAAAPSS VLLLNNGLHI DIRIDKTTAI
260 270 280 290 300
GQTDAAGVAD VVVEAALSTI LDLEDSVAAV DAEDKVVGYA NWLGILKGTL
310 320 330 340 350
TETFDKGGKS MTRGLNADRE YTGADGKPVK LHGRSLMFVR NVGHLMTNPA
360 370 380 390 400
ILWGNGKEIP EGILDAMVTT AIAVHDIKGL GANGIRNSRT GSVYIVKPKM
410 420 430 440 450
HGPAEAAFAS ELFGRVEKVL GLPENTVKLG IMDEERRTSV NLKACIAAAA
460 470 480 490 500
SRVAFINTGF LDRTGDEMHT AMYAGPMVRK ADMKGSAWLA AYERSNVLVG
510 520 530 540 550
LGLGLRGKAQ IGKGMWAMPD LMKAMLEQKI GHPKAGANTA WVPSPTAATL
560 570 580 590 600
HALHYHQVNV AEVQKQLEQT NADAERATIL ADLLQVPVVK EDKWSAAEKQ
610 620 630 640 650
QELDNNVQGI LGYVVRWVDQ GVGCSKVPDI HNVGLMEDRA TLRISSQHIA
660 670 680 690 700
NWLQHGIVTE AQVRETFERM AAVVDQQNAG DALYKPMAGH FDTSMAYQAA
710 720 730
CDLVFKGKEQ PSGYTEPLLH AWRLKVKAAA
Length:730
Mass (Da):78,381
Last modified:January 15, 2008 - v1
Checksum:i74351D7228A3262E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000884 Genomic DNA. Translation: ABX33311.1.
RefSeqiWP_012202597.1. NC_010002.1.
YP_001561696.1. NC_010002.1.

Genome annotation databases

EnsemblBacteriaiABX33311; ABX33311; Daci_0665.
GeneIDi5746218.
KEGGidac:Daci_0665.
PATRICi21635185. VBIDelAci41351_0681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000884 Genomic DNA. Translation: ABX33311.1 .
RefSeqi WP_012202597.1. NC_010002.1.
YP_001561696.1. NC_010002.1.

3D structure databases

ProteinModelPortali A9BRF0.
SMRi A9BRF0. Positions 10-729.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 398578.Daci_0665.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX33311 ; ABX33311 ; Daci_0665 .
GeneIDi 5746218.
KEGGi dac:Daci_0665.
PATRICi 21635185. VBIDelAci41351_0681.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci DACI398578:GHK3-682-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
    Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14801 / SPH-1.

Entry informationi

Entry nameiMASZ_DELAS
AccessioniPrimary (citable) accession number: A9BRF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: October 29, 2014
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3