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Protein

Malate synthase G

Gene

glcB

Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi434 – 4341MagnesiumUniRule annotation
Binding sitei434 – 4341GlyoxylateUniRule annotation
Metal bindingi462 – 4621MagnesiumUniRule annotation
Binding sitei543 – 5431Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei638 – 6381Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciDACI398578:GHK3-682-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Daci_0665
OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
Taxonomic identifieri398578 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
ProteomesiUP000000784 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 730730Malate synthase GPRO_1000130889Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi398578.Daci_0665.

Structurei

3D structure databases

ProteinModelPortaliA9BRF0.
SMRiA9BRF0. Positions 10-729.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni459 – 4624Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

A9BRF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRTQAHSL QVATELHRFI NEQVLPGTGV APEAFWKGFD AIVNDLAPRN
60 70 80 90 100
AALLAERDRL QTELDTWHKA HPGPITDMAG YQQFLTQIGY LVEQPKDAKA
110 120 130 140 150
TTTNVDAELA IQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIGE
160 170 180 190 200
DGGAEKGKGY NPVRGAKVIA FARDFLDQAA PLASGSHKDA AGYRVEAGQL
210 220 230 240 250
VVALKSGGTT GLKNTAQFVG YQGDAAAPSS VLLLNNGLHI DIRIDKTTAI
260 270 280 290 300
GQTDAAGVAD VVVEAALSTI LDLEDSVAAV DAEDKVVGYA NWLGILKGTL
310 320 330 340 350
TETFDKGGKS MTRGLNADRE YTGADGKPVK LHGRSLMFVR NVGHLMTNPA
360 370 380 390 400
ILWGNGKEIP EGILDAMVTT AIAVHDIKGL GANGIRNSRT GSVYIVKPKM
410 420 430 440 450
HGPAEAAFAS ELFGRVEKVL GLPENTVKLG IMDEERRTSV NLKACIAAAA
460 470 480 490 500
SRVAFINTGF LDRTGDEMHT AMYAGPMVRK ADMKGSAWLA AYERSNVLVG
510 520 530 540 550
LGLGLRGKAQ IGKGMWAMPD LMKAMLEQKI GHPKAGANTA WVPSPTAATL
560 570 580 590 600
HALHYHQVNV AEVQKQLEQT NADAERATIL ADLLQVPVVK EDKWSAAEKQ
610 620 630 640 650
QELDNNVQGI LGYVVRWVDQ GVGCSKVPDI HNVGLMEDRA TLRISSQHIA
660 670 680 690 700
NWLQHGIVTE AQVRETFERM AAVVDQQNAG DALYKPMAGH FDTSMAYQAA
710 720 730
CDLVFKGKEQ PSGYTEPLLH AWRLKVKAAA
Length:730
Mass (Da):78,381
Last modified:January 14, 2008 - v1
Checksum:i74351D7228A3262E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000884 Genomic DNA. Translation: ABX33311.1.
RefSeqiWP_012202597.1. NC_010002.1.
YP_001561696.1. NC_010002.1.

Genome annotation databases

EnsemblBacteriaiABX33311; ABX33311; Daci_0665.
GeneIDi5746218.
KEGGidac:Daci_0665.
PATRICi21635185. VBIDelAci41351_0681.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000884 Genomic DNA. Translation: ABX33311.1.
RefSeqiWP_012202597.1. NC_010002.1.
YP_001561696.1. NC_010002.1.

3D structure databases

ProteinModelPortaliA9BRF0.
SMRiA9BRF0. Positions 10-729.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi398578.Daci_0665.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX33311; ABX33311; Daci_0665.
GeneIDi5746218.
KEGGidac:Daci_0665.
PATRICi21635185. VBIDelAci41351_0681.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciDACI398578:GHK3-682-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14801 / SPH-1.

Entry informationi

Entry nameiMASZ_DELAS
AccessioniPrimary (citable) accession number: A9BRF0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 13, 2009
Last sequence update: January 14, 2008
Last modified: March 3, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.