A9BRF0 (MASZ_DELAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	Daci_0665

Organism

Delftia acidovorans (strain DSM 14801 / SPH-1) [Complete proteome] [HAMAP]

Taxonomic identifier

398578 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Delftia › Delftia acidovorans

Protein attributes

Sequence length	730 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 730	730	Malate synthase G HAMAP-Rule MF_00641		PRO_1000130889
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	638	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BRF0 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 74351D7228A3262E	FASTA	730	78,381
	10 20 30 40 50 60 MTDRTQAHSL QVATELHRFI NEQVLPGTGV APEAFWKGFD AIVNDLAPRN AALLAERDRL 70 80 90 100 110 120 QTELDTWHKA HPGPITDMAG YQQFLTQIGY LVEQPKDAKA TTTNVDAELA IQAGPQLVVP 130 140 150 160 170 180 ILNARYALNA ANARWGSLYD ALYGTDAIGE DGGAEKGKGY NPVRGAKVIA FARDFLDQAA 190 200 210 220 230 240 PLASGSHKDA AGYRVEAGQL VVALKSGGTT GLKNTAQFVG YQGDAAAPSS VLLLNNGLHI 250 260 270 280 290 300 DIRIDKTTAI GQTDAAGVAD VVVEAALSTI LDLEDSVAAV DAEDKVVGYA NWLGILKGTL 310 320 330 340 350 360 TETFDKGGKS MTRGLNADRE YTGADGKPVK LHGRSLMFVR NVGHLMTNPA ILWGNGKEIP 370 380 390 400 410 420 EGILDAMVTT AIAVHDIKGL GANGIRNSRT GSVYIVKPKM HGPAEAAFAS ELFGRVEKVL 430 440 450 460 470 480 GLPENTVKLG IMDEERRTSV NLKACIAAAA SRVAFINTGF LDRTGDEMHT AMYAGPMVRK 490 500 510 520 530 540 ADMKGSAWLA AYERSNVLVG LGLGLRGKAQ IGKGMWAMPD LMKAMLEQKI GHPKAGANTA 550 560 570 580 590 600 WVPSPTAATL HALHYHQVNV AEVQKQLEQT NADAERATIL ADLLQVPVVK EDKWSAAEKQ 610 620 630 640 650 660 QELDNNVQGI LGYVVRWVDQ GVGCSKVPDI HNVGLMEDRA TLRISSQHIA NWLQHGIVTE 670 680 690 700 710 720 AQVRETFERM AAVVDQQNAG DALYKPMAGH FDTSMAYQAA CDLVFKGKEQ PSGYTEPLLH 730 AWRLKVKAAA

References

[1]

"Complete sequence of Delftia acidovorans DSM 14801 / SPH-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14801 / SPH-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000884 Genomic DNA. Translation: ABX33311.1.
RefSeq	YP_001561696.1. NC_010002.1.
3D structure databases
ProteinModelPortal	A9BRF0.
SMR	A9BRF0. Positions 10-729.
ModBase	Search...
Protein-protein interaction databases
STRING	398578.Daci_0665.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX33311; ABX33311; Daci_0665.
GeneID	5746218.
KEGG	dac:Daci_0665.
PATRIC	21635185. VBIDelAci41351_0681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	SQFIENE.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	DACI398578:GHK3-763-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_DELAS

Accession

Primary (citable) accession number: A9BRF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program