Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Malate synthase G

Gene

glcB

Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: glyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei118Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Binding sitei276Acetyl-CoAUniRule annotation1
Binding sitei313Acetyl-CoAUniRule annotation1
Active sitei340Proton acceptorUniRule annotation1
Binding sitei340GlyoxylateUniRule annotation1
Metal bindingi434MagnesiumUniRule annotation1
Binding sitei434GlyoxylateUniRule annotation1
Metal bindingi462MagnesiumUniRule annotation1
Binding sitei543Acetyl-CoA; via carbonyl oxygenUniRule annotation1
Active sitei638Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processGlyoxylate bypass, Tricarboxylic acid cycle
LigandMagnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:Daci_0665
OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
Taxonomic identifieri398578 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
Proteomesi
  • UP000000784 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001308891 – 730Malate synthase GAdd BLAST730

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei624Cysteine sulfenic acid (-SOH)UniRule annotation1

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi398578.Daci_0665

Structurei

3D structure databases

ProteinModelPortaliA9BRF0
SMRiA9BRF0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni125 – 126Acetyl-CoA bindingUniRule annotation2
Regioni459 – 462Glyoxylate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QP3 Bacteria
COG2225 LUCA
HOGENOMiHOG000220740
KOiK01638
OMAiTEPVLHA

Family and domain databases

Gene3Di2.170.170.11, 1 hit
HAMAPiMF_00641 Malate_synth_G, 1 hit
InterProiView protein in InterPro
IPR011076 Malate_synth-like_sf
IPR023310 Malate_synth_G_beta_sub_dom
IPR001465 Malate_synthase
IPR006253 Malate_synthG
PANTHERiPTHR42739 PTHR42739, 1 hit
PfamiView protein in Pfam
PF01274 Malate_synthase, 1 hit
SUPFAMiSSF51645 SSF51645, 1 hit
TIGRFAMsiTIGR01345 malate_syn_G, 1 hit

Sequencei

Sequence statusi: Complete.

A9BRF0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDRTQAHSL QVATELHRFI NEQVLPGTGV APEAFWKGFD AIVNDLAPRN
60 70 80 90 100
AALLAERDRL QTELDTWHKA HPGPITDMAG YQQFLTQIGY LVEQPKDAKA
110 120 130 140 150
TTTNVDAELA IQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIGE
160 170 180 190 200
DGGAEKGKGY NPVRGAKVIA FARDFLDQAA PLASGSHKDA AGYRVEAGQL
210 220 230 240 250
VVALKSGGTT GLKNTAQFVG YQGDAAAPSS VLLLNNGLHI DIRIDKTTAI
260 270 280 290 300
GQTDAAGVAD VVVEAALSTI LDLEDSVAAV DAEDKVVGYA NWLGILKGTL
310 320 330 340 350
TETFDKGGKS MTRGLNADRE YTGADGKPVK LHGRSLMFVR NVGHLMTNPA
360 370 380 390 400
ILWGNGKEIP EGILDAMVTT AIAVHDIKGL GANGIRNSRT GSVYIVKPKM
410 420 430 440 450
HGPAEAAFAS ELFGRVEKVL GLPENTVKLG IMDEERRTSV NLKACIAAAA
460 470 480 490 500
SRVAFINTGF LDRTGDEMHT AMYAGPMVRK ADMKGSAWLA AYERSNVLVG
510 520 530 540 550
LGLGLRGKAQ IGKGMWAMPD LMKAMLEQKI GHPKAGANTA WVPSPTAATL
560 570 580 590 600
HALHYHQVNV AEVQKQLEQT NADAERATIL ADLLQVPVVK EDKWSAAEKQ
610 620 630 640 650
QELDNNVQGI LGYVVRWVDQ GVGCSKVPDI HNVGLMEDRA TLRISSQHIA
660 670 680 690 700
NWLQHGIVTE AQVRETFERM AAVVDQQNAG DALYKPMAGH FDTSMAYQAA
710 720 730
CDLVFKGKEQ PSGYTEPLLH AWRLKVKAAA
Length:730
Mass (Da):78,381
Last modified:January 15, 2008 - v1
Checksum:i74351D7228A3262E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000884 Genomic DNA Translation: ABX33311.1
RefSeqiWP_012202597.1, NC_010002.1

Genome annotation databases

EnsemblBacteriaiABX33311; ABX33311; Daci_0665
GeneIDi24118705
KEGGidac:Daci_0665

Similar proteinsi

Entry informationi

Entry nameiMASZ_DELAS
AccessioniPrimary (citable) accession number: A9BRF0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: April 25, 2018
This is version 72 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health