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A9BRF0

- MASZ_DELAS

UniProt

A9BRF0 - MASZ_DELAS

Protein

Malate synthase G

Gene

glcB

Organism
Delftia acidovorans (strain DSM 14801 / SPH-1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi434 – 4341MagnesiumUniRule annotation
    Binding sitei434 – 4341GlyoxylateUniRule annotation
    Metal bindingi462 – 4621MagnesiumUniRule annotation
    Binding sitei543 – 5431Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei638 – 6381Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciDACI398578:GHK3-682-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:Daci_0665
    OrganismiDelftia acidovorans (strain DSM 14801 / SPH-1)
    Taxonomic identifieri398578 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesComamonadaceaeDelftia
    ProteomesiUP000000784: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 730730Malate synthase GPRO_1000130889Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei624 – 6241Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi398578.Daci_0665.

    Structurei

    3D structure databases

    ProteinModelPortaliA9BRF0.
    SMRiA9BRF0. Positions 10-729.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni459 – 4624Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A9BRF0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTDRTQAHSL QVATELHRFI NEQVLPGTGV APEAFWKGFD AIVNDLAPRN    50
    AALLAERDRL QTELDTWHKA HPGPITDMAG YQQFLTQIGY LVEQPKDAKA 100
    TTTNVDAELA IQAGPQLVVP ILNARYALNA ANARWGSLYD ALYGTDAIGE 150
    DGGAEKGKGY NPVRGAKVIA FARDFLDQAA PLASGSHKDA AGYRVEAGQL 200
    VVALKSGGTT GLKNTAQFVG YQGDAAAPSS VLLLNNGLHI DIRIDKTTAI 250
    GQTDAAGVAD VVVEAALSTI LDLEDSVAAV DAEDKVVGYA NWLGILKGTL 300
    TETFDKGGKS MTRGLNADRE YTGADGKPVK LHGRSLMFVR NVGHLMTNPA 350
    ILWGNGKEIP EGILDAMVTT AIAVHDIKGL GANGIRNSRT GSVYIVKPKM 400
    HGPAEAAFAS ELFGRVEKVL GLPENTVKLG IMDEERRTSV NLKACIAAAA 450
    SRVAFINTGF LDRTGDEMHT AMYAGPMVRK ADMKGSAWLA AYERSNVLVG 500
    LGLGLRGKAQ IGKGMWAMPD LMKAMLEQKI GHPKAGANTA WVPSPTAATL 550
    HALHYHQVNV AEVQKQLEQT NADAERATIL ADLLQVPVVK EDKWSAAEKQ 600
    QELDNNVQGI LGYVVRWVDQ GVGCSKVPDI HNVGLMEDRA TLRISSQHIA 650
    NWLQHGIVTE AQVRETFERM AAVVDQQNAG DALYKPMAGH FDTSMAYQAA 700
    CDLVFKGKEQ PSGYTEPLLH AWRLKVKAAA 730
    Length:730
    Mass (Da):78,381
    Last modified:January 15, 2008 - v1
    Checksum:i74351D7228A3262E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000884 Genomic DNA. Translation: ABX33311.1.
    RefSeqiWP_012202597.1. NC_010002.1.
    YP_001561696.1. NC_010002.1.

    Genome annotation databases

    EnsemblBacteriaiABX33311; ABX33311; Daci_0665.
    GeneIDi5746218.
    KEGGidac:Daci_0665.
    PATRICi21635185. VBIDelAci41351_0681.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000884 Genomic DNA. Translation: ABX33311.1 .
    RefSeqi WP_012202597.1. NC_010002.1.
    YP_001561696.1. NC_010002.1.

    3D structure databases

    ProteinModelPortali A9BRF0.
    SMRi A9BRF0. Positions 10-729.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 398578.Daci_0665.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX33311 ; ABX33311 ; Daci_0665 .
    GeneIDi 5746218.
    KEGGi dac:Daci_0665.
    PATRICi 21635185. VBIDelAci41351_0681.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci DACI398578:GHK3-682-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Complete sequence of Delftia acidovorans DSM 14801 / SPH-1."
      Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P.
      Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14801 / SPH-1.

    Entry informationi

    Entry nameiMASZ_DELAS
    AccessioniPrimary (citable) accession number: A9BRF0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 14, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3