A9BP16 (ISPE_DELAS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Daci_5432

Organism

Delftia acidovorans (strain DSM 14801 / SPH-1) [Complete proteome] [HAMAP]

Taxonomic identifier

398578 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Comamonadaceae › Delftia

Protein attributes

Sequence length	287 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 287	287	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_1000092082
Regions
	Nucleotide binding	95 – 105	11	ATP Potential
Sites
	Active site	12	1	By similarity
	Active site	137	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BP16 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 23BB0DE4A0C65834	FASTA	287	31,597
	10 20 30 40 50 60 MRSLHDVPAP AKLNLFLHIT GRRADGYHLL QSVFMLLDWH DTLHFDRRGD GQISREDLSG 70 80 90 100 110 120 MALPADDLIV RAARALQQAT GCRAGVHIGV EKRLPAQAGM GGGSSDAAST LIALNRLWQL 130 140 150 160 170 180 NLTRRELQRI ALDLGADVPF FLCGNSAWVE GIGEHITPLE QAHALPPQRF VVVKPEAGLE 190 200 210 220 230 240 TLSIFRDATL KRDHAHATIE DFAADHYGFG QNDLQPVAER LQPEVKKAID WLNSLKLHAR 250 260 270 280 MTGSGSAVFA PLTQTIDLKD APGAWNVRVC SNLQAHPLKD WLKDESL

References

[1]

"Complete sequence of Delftia acidovorans DSM 14801 / SPH-1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Lowry S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Schleheck D., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 14801 / SPH-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000884 Genomic DNA. Translation: ABX38061.1.
RefSeq	YP_001566446.1. NC_010002.1.
3D structure databases
ProteinModelPortal	A9BP16.
ModBase	Search...
Protein-protein interaction databases
STRING	A9BP16.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5751049.
GenomeReviews	Gene locus Daci_5432 in contig CP000884_GR.
KEGG	dac:Daci_5432.
PATRIC	21644923. VBIDelAci41351_5489.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734593.
OMA	LNLFLHV.
ProtClustDB	PRK00343.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_DELAS

Accession

Primary (citable) accession number: A9BP16

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program