Reviewed, UniProtKB/Swiss-Prot A9BJB2 (SYE1_PETMO)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Pmob_0592

Organism

Petrotoga mobilis (strain DSM 10674 / SJ95) [Complete proteome] [HAMAP]

Taxonomic identifier

403833 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Petrotoga

Protein attributes

Sequence length	467 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity.
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 467	467	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000367734
Regions
	Motif	8 – 18	11	"HIGH" region HAMAP MF_00022
	Motif	230 – 234	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	233	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BJB2-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: B499133D7412F892	FASTA	467	54,997
	10 20 30 40 50 60 MIRVRFAPSP TGHLHVGGLR TALFNWYFAK KNNGKFILRI EDTDMERSKK EYEDAILEEM 70 80 90 100 110 120 KWVGLDYDEG VDKPGEYGPY RQSERLDIYK HYIDQLLNEE KAYFSVTKND EIIFEGNNLL 130 140 150 160 170 180 DKYKKNNDYS VVVKFKVNND QKISFLDDVR GTIQFDTSNI NDFVILRSNG IPVYNFTVVI 190 200 210 220 230 240 DDYLMKISHV IRGEDHISNT PKQILIYNAL SFELPKFAHL PLILGEDKSP LSKRHGEVSI 250 260 270 280 290 300 TYFREEGYLP KAILNYLSLL GWNANEQIFD YTEKYQEFDL KKVSRNPSIF DYTKLLWTNE 310 320 330 340 350 360 VHLRNDPIEE VHKSFTEWAK YTTVKIDNED SFVKKIIEAS RAKVQTLKQL YEFSKNFFVE 370 380 390 400 410 420 EFEYEEEFIE KYMKKPWFKQ VIEATIRKLS EIDEYNLANV ENTLKEIADL NITSRKNVFQ 430 440 450 460 TIRGSLLGRL VTPGLYESII ILGKNESIKR LKRALEFSNT LDINPRR

References

[1]

"Complete sequence of Petroga mobilis SJ95." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Noll K., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000879 Genomic DNA. Translation: ABX31326.1.
RefSeq	YP_001567649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5758525.
GenomeReviews	Gene locus Pmob_0592 in contig CP000879_GR.
KEGG	pmo:Pmob_0592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	WISSEYI.
Family and domain databases
HAMAP	MF_00022. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_PETMO

Accession

Primary (citable) accession number: A9BJB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)