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A9BI29

- SYI_PETMO

UniProt

A9BI29 - SYI_PETMO

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Petrotoga mobilis (strain DSM 10674 / SJ95)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylateUniRule annotation
Binding sitei596 – 5961ATPUniRule annotation
Metal bindingi886 – 8861ZincUniRule annotation
Metal bindingi889 – 8891ZincUniRule annotation
Metal bindingi906 – 9061ZincUniRule annotation
Metal bindingi909 – 9091ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMOB403833:GH51-1668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Pmob_1626
OrganismiPetrotoga mobilis (strain DSM 10674 / SJ95)
Taxonomic identifieri403833 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePetrotoga
ProteomesiUP000000789: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligasePRO_1000088551Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi403833.Pmob_1626.

Structurei

3D structure databases

ProteinModelPortaliA9BI29.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi593 – 5975"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BI29-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYKDTINLP KTSFKMKANL KEKEPQILQK WEDINIAYYL RNIRIGGTKF
60 70 80 90 100
VLHDGPPYAN GDIHMGTALN KVLKDIVLKY KTLRGYDAPY VPGWDTHGLP
110 120 130 140 150
IEHNVTTKLG EKANALSKLE IRKLCEDYAM KYVDIQRESF KRLGVIGFWD
160 170 180 190 200
KPYLTLKPEY EAKVLEILRS IVESGNIYRG TKPIYWCTEC QTALAEAEVE
210 220 230 240 250
YHDHTSDSIY VKFPLVGENN TYVIIWTTTP WTLPANVAIA VHPNFDYAKV
260 270 280 290 300
EIGNEYWIMA KELVDKTLKE AGIDDYKIID TFKGLTLEGE KARHPFVDRE
310 320 330 340 350
SLLVLADYVT LDEGTGCVHT APGHGMEDYI TGTKYNLQVI SPVDSQGYFT
360 370 380 390 400
DEAGKYKGMK IWEANKEIIK DLKENGFLVQ SGKITHSYPH CWRCKNPVIF
410 420 430 440 450
RATPQWFIDL EKNNYREKVL EEIKKVNWIP KWGENRISSM VRERPDWVIS
460 470 480 490 500
RQRAWGIPIP AIKCENCGET ILDTKIIDHV IEIIEKEGSN AWFEKEAKEL
510 520 530 540 550
LPNDYRCPKC GGSEFKKEED ILDVWIDSGS SFEAVANSRE ELKKFPVDLY
560 570 580 590 600
LEGSDQHRGW FQSSIFLSVA KHGIAPYESV LTHGFIKDEE GKKMSKSLGN
610 620 630 640 650
VVNPKDIINK YGADILRLWV ASADYRMDIK ISYNILEQQV ETYRKLRNTI
660 670 680 690 700
RFLLGNINDF DPDEDSVAYE EMLEIDQWAL MKLHNLIKNV TKAYDNYEFY
710 720 730 740 750
KVHYLINNFC TIDMSSTYLD IIKDRIYVEG KKSKLRRSAQ TVLYETAIAL
760 770 780 790 800
NKMISPILPF TAEEVYEHLN YSNKYETIFA ELWPEYKENY LSEELEEKWN
810 820 830 840 850
KIFALREDVL KALEEKRKEK FLGNSLDAKI ILNLKDDTLK QILSQYDNNW
860 870 880 890 900
IADLFIVSQF EFGNVDEGFE GRYATIKVTK AEGEKCERCW KVDPNTDNDP
910
DFPGVCPRCA RVLKEEINA
Length:919
Mass (Da):106,486
Last modified:January 15, 2008 - v1
Checksum:i712202C0E409C5DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX32320.1.
RefSeqiYP_001568643.1. NC_010003.1.

Genome annotation databases

EnsemblBacteriaiABX32320; ABX32320; Pmob_1626.
GeneIDi5756782.
KEGGipmo:Pmob_1626.
PATRICi22921064. VBIPetMob40494_1749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX32320.1 .
RefSeqi YP_001568643.1. NC_010003.1.

3D structure databases

ProteinModelPortali A9BI29.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 403833.Pmob_1626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX32320 ; ABX32320 ; Pmob_1626 .
GeneIDi 5756782.
KEGGi pmo:Pmob_1626.
PATRICi 22921064. VBIPetMob40494_1749.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMOB403833:GH51-1668-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10674 / SJ95.

Entry informationi

Entry nameiSYI_PETMO
AccessioniPrimary (citable) accession number: A9BI29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: November 26, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3