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A9BI29

- SYI_PETMO

UniProt

A9BI29 - SYI_PETMO

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Protein

Isoleucine--tRNA ligase

Gene
ileS, Pmob_1626
Organism
Petrotoga mobilis (strain DSM 10674 / SJ95)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei552 – 5521Aminoacyl-adenylate By similarity
Binding sitei596 – 5961ATP By similarity
Metal bindingi886 – 8861Zinc By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMOB403833:GH51-1668-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Pmob_1626
OrganismiPetrotoga mobilis (strain DSM 10674 / SJ95)
Taxonomic identifieri403833 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePetrotoga
ProteomesiUP000000789: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Isoleucine--tRNA ligaseUniRule annotationPRO_1000088551Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi403833.Pmob_1626.

Structurei

3D structure databases

ProteinModelPortaliA9BI29.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotationAdd
BLAST
Motifi593 – 5975"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BI29-1 [UniParc]FASTAAdd to Basket

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MDYKDTINLP KTSFKMKANL KEKEPQILQK WEDINIAYYL RNIRIGGTKF    50
VLHDGPPYAN GDIHMGTALN KVLKDIVLKY KTLRGYDAPY VPGWDTHGLP 100
IEHNVTTKLG EKANALSKLE IRKLCEDYAM KYVDIQRESF KRLGVIGFWD 150
KPYLTLKPEY EAKVLEILRS IVESGNIYRG TKPIYWCTEC QTALAEAEVE 200
YHDHTSDSIY VKFPLVGENN TYVIIWTTTP WTLPANVAIA VHPNFDYAKV 250
EIGNEYWIMA KELVDKTLKE AGIDDYKIID TFKGLTLEGE KARHPFVDRE 300
SLLVLADYVT LDEGTGCVHT APGHGMEDYI TGTKYNLQVI SPVDSQGYFT 350
DEAGKYKGMK IWEANKEIIK DLKENGFLVQ SGKITHSYPH CWRCKNPVIF 400
RATPQWFIDL EKNNYREKVL EEIKKVNWIP KWGENRISSM VRERPDWVIS 450
RQRAWGIPIP AIKCENCGET ILDTKIIDHV IEIIEKEGSN AWFEKEAKEL 500
LPNDYRCPKC GGSEFKKEED ILDVWIDSGS SFEAVANSRE ELKKFPVDLY 550
LEGSDQHRGW FQSSIFLSVA KHGIAPYESV LTHGFIKDEE GKKMSKSLGN 600
VVNPKDIINK YGADILRLWV ASADYRMDIK ISYNILEQQV ETYRKLRNTI 650
RFLLGNINDF DPDEDSVAYE EMLEIDQWAL MKLHNLIKNV TKAYDNYEFY 700
KVHYLINNFC TIDMSSTYLD IIKDRIYVEG KKSKLRRSAQ TVLYETAIAL 750
NKMISPILPF TAEEVYEHLN YSNKYETIFA ELWPEYKENY LSEELEEKWN 800
KIFALREDVL KALEEKRKEK FLGNSLDAKI ILNLKDDTLK QILSQYDNNW 850
IADLFIVSQF EFGNVDEGFE GRYATIKVTK AEGEKCERCW KVDPNTDNDP 900
DFPGVCPRCA RVLKEEINA 919
Length:919
Mass (Da):106,486
Last modified:January 15, 2008 - v1
Checksum:i712202C0E409C5DE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000879 Genomic DNA. Translation: ABX32320.1.
RefSeqiWP_012209417.1. NC_010003.1.
YP_001568643.1. NC_010003.1.

Genome annotation databases

EnsemblBacteriaiABX32320; ABX32320; Pmob_1626.
GeneIDi5756782.
KEGGipmo:Pmob_1626.
PATRICi22921064. VBIPetMob40494_1749.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000879 Genomic DNA. Translation: ABX32320.1 .
RefSeqi WP_012209417.1. NC_010003.1.
YP_001568643.1. NC_010003.1.

3D structure databases

ProteinModelPortali A9BI29.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 403833.Pmob_1626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX32320 ; ABX32320 ; Pmob_1626 .
GeneIDi 5756782.
KEGGi pmo:Pmob_1626.
PATRICi 22921064. VBIPetMob40494_1749.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMOB403833:GH51-1668-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10674 / SJ95.

Entry informationi

Entry nameiSYI_PETMO
AccessioniPrimary (citable) accession number: A9BI29
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: September 3, 2014
This is version 50 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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