Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Petrotoga mobilis (strain DSM 10674 / SJ95)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi492 – 4921MagnesiumUniRule annotation
Metal bindingi498 – 4981MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciPMOB403833:GH51-585-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:Pmob_0569
OrganismiPetrotoga mobilis (strain DSM 10674 / SJ95)
Taxonomic identifieri403833 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePetrotoga
ProteomesiUP000000789: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695Polyribonucleotide nucleotidyltransferasePRO_0000329756Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi403833.Pmob_0569.

Structurei

3D structure databases

ProteinModelPortaliA9BH73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini559 – 61860KHUniRule annotationAdd
BLAST
Domaini628 – 69164S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BH73-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVLEKELFG RKLRIEHGKV AKQSLGSVML TFNESTILVT ADASEETVKG
60 70 80 90 100
QDFFPLTVEF QEKFYAVGKI PGGFIKREGK PSDEAILAAR LIDRPIRPLF
110 120 130 140 150
PENFFNEVQV ITTVFSMLNG DSIETWGITG ASLALNLSPI PFNGIVAGVR
160 170 180 190 200
IGYVDGQFIA FPTQEQLKNS KIDMVVAGTK DAVTMVEGES LEVSEEEMVE
210 220 230 240 250
ALMFAQEKIK EIIAIEEEFL SELNIEKWEV QKEIVPEEFI EDYLSLIDEE
260 270 280 290 300
ELKKVLLTKG KKNRDKVISE YKKNVMKKFE EIFLEKWSVA FFEDKKRFLE
310 320 330 340 350
NAFEEKLQDS MRKMIINENK RVDGRTCDEI RDITCEVGLI PRVHGSALFT
360 370 380 390 400
RGETQSLGTV TLGAPLDVQV LDTIFSDEEK RFMLHYNFPP FSTGEVKRLR
410 420 430 440 450
GVSRREIGHG HLAERAHKNL IPSDEEFPYT IRVVSDILES NGSSSMASVC
460 470 480 490 500
SASLALMDAG VPIQKHVAGI AMGLIFENDN FVVLTDILGM EDHLGDMDFK
510 520 530 540 550
VAGTRDGITA FQMDVKTSQV NKEVLQKALE KAKIARLKIL DKMYETIPQP
560 570 580 590 600
RKELSPYVPI MKVFKIPVSK IGEVIGPGGK NIKEISELYN VEVYIEDDGK
610 620 630 640 650
VKVTGHNANK VDEAINHIQN LIAQVEKGGI FEGTVKRVEK YGIFVEVLPG
660 670 680 690
KVGMLHVSNL KDKLESFKIG DKVKVEVMKV EDQGKFQLKQ LKEEN
Length:695
Mass (Da):78,198
Last modified:January 15, 2008 - v1
Checksum:iE9F95A0FC9020DF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX31303.1.
RefSeqiYP_001567626.1. NC_010003.1.

Genome annotation databases

EnsemblBacteriaiABX31303; ABX31303; Pmob_0569.
GeneIDi5757941.
KEGGipmo:Pmob_0569.
PATRICi22918712. VBIPetMob40494_0596.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX31303.1.
RefSeqiYP_001567626.1. NC_010003.1.

3D structure databases

ProteinModelPortaliA9BH73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi403833.Pmob_0569.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX31303; ABX31303; Pmob_0569.
GeneIDi5757941.
KEGGipmo:Pmob_0569.
PATRICi22918712. VBIPetMob40494_0596.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciPMOB403833:GH51-585-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF13014. KH_3. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10674 / SJ95.

Entry informationi

Entry nameiPNP_PETMO
AccessioniPrimary (citable) accession number: A9BH73
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: January 15, 2008
Last modified: February 4, 2015
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.