A9BGT8 (SYE2_PETMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Pmob_1830

Organism

Petrotoga mobilis (strain DSM 10674 / SJ95) [Complete proteome] [HAMAP]

Taxonomic identifier

403833 [NCBI]

Taxonomic lineage

Bacteria › Thermotogae › Thermotogales › Thermotogaceae › Petrotoga

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367743
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	254 – 258	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	257	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BGT8 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 8409936946E89202	FASTA	488	56,768
	10 20 30 40 50 60 MISNTVRTRF APSPTGYLHV GGARTALFNY LFSKKNEGKF ILRIEDTDIE RSTKESEDQL 70 80 90 100 110 120 INTLKWLNLN WDEGPIIGGD YEPYRQSERL EIYQRRAKEL IEKGKAYEAY ISPEEIEEVK 130 140 150 160 170 180 NQLISEGKPP HYTYDLISKY NTKERIEEYN YKGLKPVIFL KMPQKDYELD DKIKGKVIFK 190 200 210 220 230 240 KGSIGDFIIL RSNGIPTYNF AVVVDDIEMK ITHVIRGDDH LPNTLRQMAI YEAFEVEPPI 250 260 270 280 290 300 FAHVSMILGP DGKKLSKRHG ATSVEEFMVQ GYLPEAVDNY LALLGWSHPE GKEILPLSEI 310 320 330 340 350 360 IDNFTLDRVN SSPAIFDEKK LKWMNGQYLR NKPLEEIYQL AEPFVTGSKL LSKEQYEVNK 370 380 390 400 410 420 KWVIKALETI LSSVEILSEI PEKMEVFLKD VYPDTNDPEF KEYFDKRGVK EAINLIYKYF 430 440 450 460 470 480 NEDDRWDTQT ITENLKNAMN EANPQKKPFY MSLRKILTDS FHGPDLINTI FLLGRNTVLE RLQRVIEI

References

[1]

"Complete sequence of Petroga mobilis SJ95." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. , Kyrpides N., Mikhailova N., Noll K., Richardson P. Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 10674 / SJ95.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000879 Genomic DNA. Translation: ABX32519.1.
RefSeq	YP_001568842.1. NC_010003.1.
3D structure databases
ProteinModelPortal	A9BGT8.
SMR	A9BGT8. Positions 5-486.
ModBase	Search...
Protein-protein interaction databases
STRING	A9BGT8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5758046.
GenomeReviews	Gene locus Pmob_1830 in contig CP000879_GR.
KEGG	pmo:Pmob_1830.
PATRIC	22921502. VBIPetMob40494_1962.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	FEDLLHG.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PMOB403833:PMOB_1830-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K09698.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_PETMO

Accession

Primary (citable) accession number: A9BGT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program