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Protein

Glutamate--tRNA ligase 2

Gene

gltX2

Organism
Petrotoga mobilis (strain DSM 10674 / SJ95)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei257 – 2571ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPMOB403833:GH51-1878-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligase 2UniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetase 2UniRule annotation
Short name:
GluRS 2UniRule annotation
Gene namesi
Name:gltX2UniRule annotation
Ordered Locus Names:Pmob_1830
OrganismiPetrotoga mobilis (strain DSM 10674 / SJ95)
Taxonomic identifieri403833 [NCBI]
Taxonomic lineageiBacteriaThermotogaeThermotogalesThermotogaceaePetrotoga
ProteomesiUP000000789 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 488488Glutamate--tRNA ligase 2PRO_0000367743Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi403833.Pmob_1830.

Structurei

3D structure databases

SMRiA9BGT8. Positions 5-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi12 – 2211"HIGH" regionAdd
BLAST
Motifi254 – 2585"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK09698.
OMAiVTYDRKC.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BGT8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISNTVRTRF APSPTGYLHV GGARTALFNY LFSKKNEGKF ILRIEDTDIE
60 70 80 90 100
RSTKESEDQL INTLKWLNLN WDEGPIIGGD YEPYRQSERL EIYQRRAKEL
110 120 130 140 150
IEKGKAYEAY ISPEEIEEVK NQLISEGKPP HYTYDLISKY NTKERIEEYN
160 170 180 190 200
YKGLKPVIFL KMPQKDYELD DKIKGKVIFK KGSIGDFIIL RSNGIPTYNF
210 220 230 240 250
AVVVDDIEMK ITHVIRGDDH LPNTLRQMAI YEAFEVEPPI FAHVSMILGP
260 270 280 290 300
DGKKLSKRHG ATSVEEFMVQ GYLPEAVDNY LALLGWSHPE GKEILPLSEI
310 320 330 340 350
IDNFTLDRVN SSPAIFDEKK LKWMNGQYLR NKPLEEIYQL AEPFVTGSKL
360 370 380 390 400
LSKEQYEVNK KWVIKALETI LSSVEILSEI PEKMEVFLKD VYPDTNDPEF
410 420 430 440 450
KEYFDKRGVK EAINLIYKYF NEDDRWDTQT ITENLKNAMN EANPQKKPFY
460 470 480
MSLRKILTDS FHGPDLINTI FLLGRNTVLE RLQRVIEI
Length:488
Mass (Da):56,768
Last modified:January 14, 2008 - v1
Checksum:i8409936946E89202
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX32519.1.
RefSeqiYP_001568842.1. NC_010003.1.

Genome annotation databases

EnsemblBacteriaiABX32519; ABX32519; Pmob_1830.
KEGGipmo:Pmob_1830.
PATRICi22921502. VBIPetMob40494_1962.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000879 Genomic DNA. Translation: ABX32519.1.
RefSeqiYP_001568842.1. NC_010003.1.

3D structure databases

SMRiA9BGT8. Positions 5-486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi403833.Pmob_1830.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX32519; ABX32519; Pmob_1830.
KEGGipmo:Pmob_1830.
PATRICi22921502. VBIPetMob40494_1962.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK09698.
OMAiVTYDRKC.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciPMOB403833:GH51-1878-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 10674 / SJ95.

Entry informationi

Entry nameiSYE2_PETMO
AccessioniPrimary (citable) accession number: A9BGT8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 23, 2009
Last sequence update: January 14, 2008
Last modified: March 31, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.