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A9BF22 (SYL_PETMO) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Pmob_0344
OrganismPetrotoga mobilis (strain DSM 10674 / SJ95) [Complete proteome] [HAMAP]
Taxonomic identifier403833 [NCBI]
Taxonomic lineageBacteriaThermotogaeThermotogalesThermotogaceaePetrotoga

Protein attributes

Sequence length828 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 828828Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199217

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif582 – 5865"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5851ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9BF22 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: F65300E294F9C3E0

FASTA82896,100
        10         20         30         40         50         60 
MEKTYNPQEI ERKWQRNWQE KDAFKVSNEE YHKKYYDLVM FPYPSGTLHV GHVKNYVIGD 

        70         80         90        100        110        120 
VIARYKRMRG YNVMHPFGFD AFGLPAENAA IEKGNIHPED WTMQNINIIR NQIKKLGISY 

       130        140        150        160        170        180 
NWEREVITCK EDYYKWTQWI FLQLYKNGLA YKKKAPVNWC PNCKTVLANE QVVNGKCERC 

       190        200        210        220        230        240 
GTVVEIKQLE QWYFKITDYA EKLLYDLEKL SGWPENVKIM QKNWIGKSVG AEVEFNLDNG 

       250        260        270        280        290        300 
KGSLRVFTTR PDTLWGVTFM ALAPESPLVE ELTTPENSEK VNQFLQRVSL QDRFKRTAEG 

       310        320        330        340        350        360 
AEKEGVFTGS YAINPVNGEK IPIYVANYIL YEYGTGAIMA VPAHDQRDFE FAKKYDLPIR 

       370        380        390        400        410        420 
IVIMPEGDEL TEENLEKAYI GEGVLVNSGE FTGLDNQTAI REVSQWLEDK KIGKVVTQYK 

       430        440        450        460        470        480 
LRDWLISRQR YWGAPIPVVY CEKCGVVPVP EKDLPVKLPR DVAFEPTGKS PLIDHPDFKE 

       490        500        510        520        530        540 
TTCPKCGGKA KREVDTMDTF VDSSWYYLRY VNPKLEDKPF NKEDVDNWLP VDQYIGGVEH 

       550        560        570        580        590        600 
AILHLLYSRF ITKVLKDLGY VSFDEPFKNL FTQGMIYRNG AKMSKSKGNV VSPEEMIEKY 

       610        620        630        640        650        660 
GTDALRTYIL FMAPPERDAE WNDSGIEGTY RFLNKVWNTY MKIQDKIIHL ENKPNYPLKN 

       670        680        690        700        710        720 
KSEKDLRRKL HQTIEKITSD IEGNFQFNTA VSSLMELLNE LNSYLNNTDD KDWNLNLLKE 

       730        740        750        760        770        780 
FSEDFVLMLS PIAPHISEEL WKNFGKDEFI FKASWPEIDK NALKAEEITL AVQINGKLRA 

       790        800        810        820 
QITVDVSLNE DEVKSYALED DKVQKYISGK KIQKIIYVPK KIINIVVK 

« Hide

References

[1]"Complete sequence of Petroga mobilis SJ95."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Meincke L., Brettin T., Bruce D., Detter J.C., Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L. expand/collapse author list , Kyrpides N., Mikhailova N., Noll K., Richardson P.
Submitted (NOV-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 10674 / SJ95.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000879 Genomic DNA. Translation: ABX31086.1.
RefSeqYP_001567409.1. NC_010003.1.

3D structure databases

ProteinModelPortalA9BF22.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING403833.Pmob_0344.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX31086; ABX31086; Pmob_0344.
GeneID5758364.
KEGGpmo:Pmob_0344.
PATRIC22918226. VBIPetMob40494_0360.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycPMOB403833:GH51-352-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_PETMO
AccessionPrimary (citable) accession number: A9BF22
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries