A9BEI3 (GLO2_PROM4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	P9211_05621

Organism

Prochlorococcus marinus (strain MIT 9211) [Complete proteome] [HAMAP]

Taxonomic identifier

93059 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity. HAMAP MF_01374
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP MF_01374
Subunit structure	Monomer By similarity. HAMAP MF_01374
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Hydroxyacylglutathione hydrolase HAMAP MF_01374		PRO_1000144777
Sites
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 1 By similarity
	Metal binding	63	1	Zinc 2 By similarity
	Metal binding	64	1	Zinc 2 By similarity
	Metal binding	118	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 1 By similarity
	Metal binding	143	1	Zinc 2 By similarity
	Metal binding	181	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BEI3 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 265492D9DFB7B5D0	FASTA	253	28,798
	10 20 30 40 50 60 MTQEKKDFTI HALPVLKDNI IWIWEASGQA VVVDPAVSEP VKEFLSQNNL ALNSVLQTHH 70 80 90 100 110 120 HDDHIGGTRD LISNWPSASI IACKTDLERI PFQTHSVTDQ EVFTLFGYSV KVLEVPGHTR 130 140 150 160 170 180 GHVAYYLSDT NADNRNPALF CGDTLFAGGC GRLFEGTPHE MFKSLKLLNS LPSNTKIYCA 190 200 210 220 230 240 HEYTESNLHW ANHLYPEDLL IKKRLEYVSS QRANGLLSLP STIAEERKTN LFFRARTLEQ 250 FSQFRKHKDN WMS

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9211.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000878 Genomic DNA. Translation: ABX08493.1.
RefSeq	YP_001550447.1. NC_009976.1.
3D structure databases
ProteinModelPortal	A9BEI3.
ModBase	Search...
Protein-protein interaction databases
STRING	A9BEI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5731168.
GenomeReviews	Gene locus P9211_05621 in contig CP000878_GR.
KEGG	pmj:P9211_05621.
PATRIC	22986618. VBIProMar136502_0592.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG753931.
OMA	IGCGRVF.
PhylomeDB	A9BEI3.
ProtClustDB	CLSK919284.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2. [Tree]
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
KO	K01069.
PANTHER	PTHR11935:SF7. PTHR11935:SF7. 1 hit.
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_PROM4

Accession

Primary (citable) accession number: A9BEI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program