ID   UCRI_PROM4              Reviewed;         178 AA.
AC   A9BE84;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   27-MAR-2024, entry version 101.
DE   RecName: Full=Cytochrome b6-f complex iron-sulfur subunit {ECO:0000255|HAMAP-Rule:MF_01335};
DE            EC=7.1.1.6 {ECO:0000255|HAMAP-Rule:MF_01335};
DE   AltName: Full=Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein {ECO:0000255|HAMAP-Rule:MF_01335};
DE            Short=ISP {ECO:0000255|HAMAP-Rule:MF_01335};
DE            Short=RISP {ECO:0000255|HAMAP-Rule:MF_01335};
DE   AltName: Full=Rieske iron-sulfur protein {ECO:0000255|HAMAP-Rule:MF_01335};
GN   Name=petC {ECO:0000255|HAMAP-Rule:MF_01335};
GN   OrderedLocusNames=P9211_04631;
OS   Prochlorococcus marinus (strain MIT 9211).
OC   Bacteria; Cyanobacteriota; Cyanophyceae; Synechococcales;
OC   Prochlorococcaceae; Prochlorococcus.
OX   NCBI_TaxID=93059;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MIT 9211;
RX   PubMed=18159947; DOI=10.1371/journal.pgen.0030231;
RA   Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S.,
RA   Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M.,
RA   Richardson P., Chisholm S.W.;
RT   "Patterns and implications of gene gain and loss in the evolution of
RT   Prochlorococcus.";
RL   PLoS Genet. 3:2515-2528(2007).
CC   -!- FUNCTION: Component of the cytochrome b6-f complex, which mediates
CC       electron transfer between photosystem II (PSII) and photosystem I
CC       (PSI), cyclic electron flow around PSI, and state transitions.
CC       {ECO:0000255|HAMAP-Rule:MF_01335}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a plastoquinol + 2 H(+)(in) + 2 oxidized [plastocyanin] = a
CC         plastoquinone + 4 H(+)(out) + 2 reduced [plastocyanin];
CC         Xref=Rhea:RHEA:22148, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562,
CC         Rhea:RHEA-COMP:10039, Rhea:RHEA-COMP:10040, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17757, ChEBI:CHEBI:29036, ChEBI:CHEBI:49552,
CC         ChEBI:CHEBI:62192; EC=7.1.1.6; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01335};
CC   -!- COFACTOR:
CC       Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01335};
CC       Note=Binds 1 [2Fe-2S] cluster per subunit. {ECO:0000255|HAMAP-
CC       Rule:MF_01335};
CC   -!- SUBUNIT: The 4 large subunits of the cytochrome b6-f complex are
CC       cytochrome b6, subunit IV (17 kDa polypeptide, PetD), cytochrome f and
CC       the Rieske protein, while the 4 small subunits are PetG, PetL, PetM and
CC       PetN. The complex functions as a dimer. {ECO:0000255|HAMAP-
CC       Rule:MF_01335}.
CC   -!- SUBCELLULAR LOCATION: Cellular thylakoid membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01335}; Single-pass membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01335}. Note=The transmembrane helix obliquely spans the
CC       membrane in one monomer, and its extrinsic C-terminal domain is part of
CC       the other monomer. {ECO:0000255|HAMAP-Rule:MF_01335}.
CC   -!- MISCELLANEOUS: The Rieske iron-sulfur protein is a high potential 2Fe-
CC       2S protein.
CC   -!- SIMILARITY: Belongs to the Rieske iron-sulfur protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_01335}.
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DR   EMBL; CP000878; ABX08394.1; -; Genomic_DNA.
DR   RefSeq; WP_012195017.1; NC_009976.1.
DR   AlphaFoldDB; A9BE84; -.
DR   SMR; A9BE84; -.
DR   STRING; 93059.P9211_04631; -.
DR   KEGG; pmj:P9211_04631; -.
DR   eggNOG; COG0723; Bacteria.
DR   HOGENOM; CLU_055690_8_0_3; -.
DR   OrthoDB; 9767869at2; -.
DR   Proteomes; UP000000788; Chromosome.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:UniProt.
DR   GO; GO:0031676; C:plasma membrane-derived thylakoid membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR   GO; GO:0045158; F:electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009496; F:plastoquinol--plastocyanin reductase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0015979; P:photosynthesis; IEA:UniProtKB-UniRule.
DR   CDD; cd03471; Rieske_cytochrome_b6f; 1.
DR   Gene3D; 2.102.10.10; Rieske [2Fe-2S] iron-sulphur domain; 1.
DR   Gene3D; 1.20.5.700; Single helix bin; 1.
DR   HAMAP; MF_01335; Cytb6_f_Rieske; 1.
DR   InterPro; IPR023960; Cyt_b6_f_Rieske.
DR   InterPro; IPR017941; Rieske_2Fe-2S.
DR   InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR   InterPro; IPR014349; Rieske_Fe-S_prot.
DR   InterPro; IPR005805; Rieske_Fe-S_prot_C.
DR   InterPro; IPR006311; TAT_signal.
DR   PANTHER; PTHR10134; CYTOCHROME B-C1 COMPLEX SUBUNIT RIESKE, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR10134:SF50; CYTOCHROME B6-F COMPLEX IRON-SULFUR SUBUNIT, CHLOROPLASTIC; 1.
DR   Pfam; PF00355; Rieske; 1.
DR   PRINTS; PR00162; RIESKE.
DR   SUPFAM; SSF50022; ISP domain; 1.
DR   PROSITE; PS51296; RIESKE; 1.
DR   PROSITE; PS51318; TAT; 1.
PE   3: Inferred from homology;
KW   2Fe-2S; Disulfide bond; Electron transport; Iron; Iron-sulfur; Membrane;
KW   Metal-binding; Reference proteome; Thylakoid; Translocase; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..178
FT                   /note="Cytochrome b6-f complex iron-sulfur subunit"
FT                   /id="PRO_1000142569"
FT   TRANSMEM        20..42
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   DOMAIN          71..161
FT                   /note="Rieske"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   BINDING         107
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   BINDING         109
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   BINDING         125
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   BINDING         128
FT                   /ligand="[2Fe-2S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:190135"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
FT   DISULFID        112..127
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01335"
SQ   SEQUENCE   178 AA;  18858 MW;  2552824A7D1DB4E4 CRC64;
     MTQMTPSDVP SMGRRQFMNL LTFGTATGVA LGALYPVANY FMPLRAGGGG GGTSAKDELG
     NPITASGWLS NHPAGDRSLV QGLKGDPTYL IVEGSEAIGN FGINAICTHL GCVVPWNSGA
     NKYICPCHGS QYDANGKVVR GPAPLSLALA HIDVEDDKVF VSQWAETDFR TGEKPWWT
//