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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9211)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851Aminoacyl-adenylateUniRule annotation
Binding sitei629 – 6291ATPUniRule annotation
Metal bindingi939 – 9391ZincUniRule annotation
Metal bindingi942 – 9421ZincUniRule annotation
Metal bindingi959 – 9591ZincUniRule annotation
Metal bindingi962 – 9621ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR93059:GHJV-265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:P9211_02611
OrganismiProchlorococcus marinus (strain MIT 9211)
Taxonomic identifieri93059 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000000788 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Isoleucine--tRNA ligasePRO_1000189186Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi93059.P9211_02611.

Structurei

3D structure databases

ProteinModelPortaliA9BDK6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionAdd
BLAST
Motifi626 – 6305"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BDK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKETRQGEQ NRPSYKESLN LLKTSFGMRA NATLREPELQ NFWADKGIDL
60 70 80 90 100
ELGLANKGPS FTLHDGPPYA NGALHMGHAL NKVLKDIINK FHIMRGHKVH
110 120 130 140 150
FVPGWDCHGL PIELKVLQTL SNKERQALTP IQLRKKAAAY ASKQVKGQME
160 170 180 190 200
GFKRWGIWGN WNKPYLTLQK EYESAQVKLF GQMVLKGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPEAHTSP SVYVAFSVIH LPQSLSENLI KQGLELPGDE
260 270 280 290 300
VELSKRLKVC IWTTTPWTLP ANAAVSVNSN LDYSFVRHEE KGQILIFATE
310 320 330 340 350
LLEEVSEILG ISFKQVANAK GESLKGILYK HPLYERTAPI VLGGSYITTQ
360 370 380 390 400
SGTGLVHTAP GHGIDDFKTG LQNNLEVFCP VDEKGIFTSE AGKFEGLNVL
410 420 430 440 450
KDANKEIISS LEISGSLLKE LPYVHKYPYD WRTKKPTIFR ATEQWFASVE
460 470 480 490 500
GFREDALKAI DDVEWLPESG RNRIQSMVRE RGDWCISRQR TWGIPIPVFY
510 520 530 540 550
KKNTNEILLN KETIDHIENL FAQYGADIWW EFDESKLLPP SLASESHHWQ
560 570 580 590 600
KGVDTMDVWF DSGSSWASVS CQRDELGYPA DLYLEGTDQH RGWFQSSLLT
610 620 630 640 650
SVAVNGHAPY KKVLTHGFAL DENGRKMSKS LGNIIDPTVI INGGTNKKTD
660 670 680 690 700
PAYGADVLRL WVSSVDYSVD VPIGSNILKQ LSDVYRKVRN TSRYLLGNLH
710 720 730 740 750
DFDPKKNSVD IDNLPILDKW MLNRTAEVID EITLAFEKYE FSRFFQLLQS
760 770 780 790 800
FCTVDLSNFY LDIAKDRLYV SAPDDPRRRA CQTVMALIIE KLAGLISPVL
810 820 830 840 850
CHMAEDIWQN IPYQLSEESV FKRGWPVSPS NWKRASLTEP ISLIRELRTS
860 870 880 890 900
VNRVLEGCRN RQELGSSLEA GLRLEITNQG LLDAIDFLAK NGDHDVDCIR
910 920 930 940 950
DWFLVSQLQI GGEPWAEVLI SQQFELGVIE ISNARGYKCE RCWHYEQDIG
960
SCSEHPTLCG RCVTVMNRL
Length:969
Mass (Da):109,816
Last modified:January 15, 2008 - v1
Checksum:i9C4CAAB0CD942EAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000878 Genomic DNA. Translation: ABX08192.1.
RefSeqiWP_012194817.1. NC_009976.1.

Genome annotation databases

EnsemblBacteriaiABX08192; ABX08192; P9211_02611.
KEGGipmj:P9211_02611.
PATRICi22985966. VBIProMar136502_0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000878 Genomic DNA. Translation: ABX08192.1.
RefSeqiWP_012194817.1. NC_009976.1.

3D structure databases

ProteinModelPortaliA9BDK6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi93059.P9211_02611.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX08192; ABX08192; P9211_02611.
KEGGipmj:P9211_02611.
PATRICi22985966. VBIProMar136502_0279.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciPMAR93059:GHJV-265-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9211.

Entry informationi

Entry nameiSYI_PROM4
AccessioniPrimary (citable) accession number: A9BDK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: July 22, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.