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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Prochlorococcus marinus (strain MIT 9211)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei585Aminoacyl-adenylateUniRule annotation1
Binding sitei629ATPUniRule annotation1
Metal bindingi939ZincUniRule annotation1
Metal bindingi942ZincUniRule annotation1
Metal bindingi959ZincUniRule annotation1
Metal bindingi962ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:P9211_02611
OrganismiProchlorococcus marinus (strain MIT 9211)
Taxonomic identifieri93059 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesProchloraceaeProchlorococcus
Proteomesi
  • UP000000788 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891861 – 969Isoleucine--tRNA ligaseAdd BLAST969

Proteomic databases

PRIDEiA9BDK6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi93059.P9211_02611

Structurei

3D structure databases

ProteinModelPortaliA9BDK6
SMRiA9BDK6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi68 – 78"HIGH" regionAdd BLAST11
Motifi626 – 630"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A9BDK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKETRQGEQ NRPSYKESLN LLKTSFGMRA NATLREPELQ NFWADKGIDL
60 70 80 90 100
ELGLANKGPS FTLHDGPPYA NGALHMGHAL NKVLKDIINK FHIMRGHKVH
110 120 130 140 150
FVPGWDCHGL PIELKVLQTL SNKERQALTP IQLRKKAAAY ASKQVKGQME
160 170 180 190 200
GFKRWGIWGN WNKPYLTLQK EYESAQVKLF GQMVLKGYIY RGLKPVHWSP
210 220 230 240 250
SSRTALAEAE LEYPEAHTSP SVYVAFSVIH LPQSLSENLI KQGLELPGDE
260 270 280 290 300
VELSKRLKVC IWTTTPWTLP ANAAVSVNSN LDYSFVRHEE KGQILIFATE
310 320 330 340 350
LLEEVSEILG ISFKQVANAK GESLKGILYK HPLYERTAPI VLGGSYITTQ
360 370 380 390 400
SGTGLVHTAP GHGIDDFKTG LQNNLEVFCP VDEKGIFTSE AGKFEGLNVL
410 420 430 440 450
KDANKEIISS LEISGSLLKE LPYVHKYPYD WRTKKPTIFR ATEQWFASVE
460 470 480 490 500
GFREDALKAI DDVEWLPESG RNRIQSMVRE RGDWCISRQR TWGIPIPVFY
510 520 530 540 550
KKNTNEILLN KETIDHIENL FAQYGADIWW EFDESKLLPP SLASESHHWQ
560 570 580 590 600
KGVDTMDVWF DSGSSWASVS CQRDELGYPA DLYLEGTDQH RGWFQSSLLT
610 620 630 640 650
SVAVNGHAPY KKVLTHGFAL DENGRKMSKS LGNIIDPTVI INGGTNKKTD
660 670 680 690 700
PAYGADVLRL WVSSVDYSVD VPIGSNILKQ LSDVYRKVRN TSRYLLGNLH
710 720 730 740 750
DFDPKKNSVD IDNLPILDKW MLNRTAEVID EITLAFEKYE FSRFFQLLQS
760 770 780 790 800
FCTVDLSNFY LDIAKDRLYV SAPDDPRRRA CQTVMALIIE KLAGLISPVL
810 820 830 840 850
CHMAEDIWQN IPYQLSEESV FKRGWPVSPS NWKRASLTEP ISLIRELRTS
860 870 880 890 900
VNRVLEGCRN RQELGSSLEA GLRLEITNQG LLDAIDFLAK NGDHDVDCIR
910 920 930 940 950
DWFLVSQLQI GGEPWAEVLI SQQFELGVIE ISNARGYKCE RCWHYEQDIG
960
SCSEHPTLCG RCVTVMNRL
Length:969
Mass (Da):109,816
Last modified:January 15, 2008 - v1
Checksum:i9C4CAAB0CD942EAF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000878 Genomic DNA Translation: ABX08192.1
RefSeqiWP_012194817.1, NC_009976.1

Genome annotation databases

EnsemblBacteriaiABX08192; ABX08192; P9211_02611
KEGGipmj:P9211_02611

Similar proteinsi

Entry informationi

Entry nameiSYI_PROM4
AccessioniPrimary (citable) accession number: A9BDK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health