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A9BDK6

- SYI_PROM4

UniProt

A9BDK6 - SYI_PROM4

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Protein

Isoleucine--tRNA ligase

Gene
ileS, P9211_02611
Organism
Prochlorococcus marinus (strain MIT 9211)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei585 – 5851Aminoacyl-adenylate By similarity
Binding sitei629 – 6291ATP By similarity
Metal bindingi939 – 9391Zinc By similarity
Metal bindingi942 – 9421Zinc By similarity
Metal bindingi959 – 9591Zinc By similarity
Metal bindingi962 – 9621Zinc By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR93059:GHJV-265-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:P9211_02611
OrganismiProchlorococcus marinus (strain MIT 9211)
Taxonomic identifieri93059 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaProchloralesProchlorococcaceaeProchlorococcus
ProteomesiUP000000788: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 969969Isoleucine--tRNA ligaseUniRule annotationPRO_1000189186Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi93059.P9211_02611.

Structurei

3D structure databases

ProteinModelPortaliA9BDK6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi68 – 7811"HIGH" regionUniRule annotationAdd
BLAST
Motifi626 – 6305"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiEVMDEIT.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9BDK6-1 [UniParc]FASTAAdd to Basket

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MTKETRQGEQ NRPSYKESLN LLKTSFGMRA NATLREPELQ NFWADKGIDL    50
ELGLANKGPS FTLHDGPPYA NGALHMGHAL NKVLKDIINK FHIMRGHKVH 100
FVPGWDCHGL PIELKVLQTL SNKERQALTP IQLRKKAAAY ASKQVKGQME 150
GFKRWGIWGN WNKPYLTLQK EYESAQVKLF GQMVLKGYIY RGLKPVHWSP 200
SSRTALAEAE LEYPEAHTSP SVYVAFSVIH LPQSLSENLI KQGLELPGDE 250
VELSKRLKVC IWTTTPWTLP ANAAVSVNSN LDYSFVRHEE KGQILIFATE 300
LLEEVSEILG ISFKQVANAK GESLKGILYK HPLYERTAPI VLGGSYITTQ 350
SGTGLVHTAP GHGIDDFKTG LQNNLEVFCP VDEKGIFTSE AGKFEGLNVL 400
KDANKEIISS LEISGSLLKE LPYVHKYPYD WRTKKPTIFR ATEQWFASVE 450
GFREDALKAI DDVEWLPESG RNRIQSMVRE RGDWCISRQR TWGIPIPVFY 500
KKNTNEILLN KETIDHIENL FAQYGADIWW EFDESKLLPP SLASESHHWQ 550
KGVDTMDVWF DSGSSWASVS CQRDELGYPA DLYLEGTDQH RGWFQSSLLT 600
SVAVNGHAPY KKVLTHGFAL DENGRKMSKS LGNIIDPTVI INGGTNKKTD 650
PAYGADVLRL WVSSVDYSVD VPIGSNILKQ LSDVYRKVRN TSRYLLGNLH 700
DFDPKKNSVD IDNLPILDKW MLNRTAEVID EITLAFEKYE FSRFFQLLQS 750
FCTVDLSNFY LDIAKDRLYV SAPDDPRRRA CQTVMALIIE KLAGLISPVL 800
CHMAEDIWQN IPYQLSEESV FKRGWPVSPS NWKRASLTEP ISLIRELRTS 850
VNRVLEGCRN RQELGSSLEA GLRLEITNQG LLDAIDFLAK NGDHDVDCIR 900
DWFLVSQLQI GGEPWAEVLI SQQFELGVIE ISNARGYKCE RCWHYEQDIG 950
SCSEHPTLCG RCVTVMNRL 969
Length:969
Mass (Da):109,816
Last modified:January 15, 2008 - v1
Checksum:i9C4CAAB0CD942EAF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000878 Genomic DNA. Translation: ABX08192.1.
RefSeqiYP_001550146.1. NC_009976.1.

Genome annotation databases

EnsemblBacteriaiABX08192; ABX08192; P9211_02611.
GeneIDi5731556.
KEGGipmj:P9211_02611.
PATRICi22985966. VBIProMar136502_0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000878 Genomic DNA. Translation: ABX08192.1 .
RefSeqi YP_001550146.1. NC_009976.1.

3D structure databases

ProteinModelPortali A9BDK6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 93059.P9211_02611.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX08192 ; ABX08192 ; P9211_02611 .
GeneIDi 5731556.
KEGGi pmj:P9211_02611.
PATRICi 22985966. VBIProMar136502_0279.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi EVMDEIT.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR93059:GHJV-265-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: MIT 9211.

Entry informationi

Entry nameiSYI_PROM4
AccessioniPrimary (citable) accession number: A9BDK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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