A9BCK1 (EFG_PROM4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Elongation factor G
Short name=EF-G

Gene names

Name:	fusA
Ordered Locus Names:	P9211_16321

Organism

Prochlorococcus marinus (strain MIT 9211) [Complete proteome] [HAMAP]

Taxonomic identifier

93059 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Prochlorophytes › Prochlorococcaceae › Prochlorococcus

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome By similarity. HAMAP MF_00054_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00054_B.
Sequence similarities	Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Elongation factor
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro translation elongation factor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 691	691	Elongation factor G HAMAP MF_00054_B		PRO_1000091749
Regions
	Nucleotide binding	17 – 24	8	GTP By similarity
	Nucleotide binding	81 – 85	5	GTP By similarity
	Nucleotide binding	135 – 138	4	GTP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BCK1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: DEB65B855E473867	FASTA	691	75,402
	10 20 30 40 50 60 MARPFPLERV RNIGIAAHID AGKTTTTERI LFYSGVVHKI GEVHDGAAVT DWMAQERERG 70 80 90 100 110 120 ITITAAAIST SWQDHRINII DTPGHVDFTI EVERSMRVLD GVIAVFCAVG GVQPQSETVW 130 140 150 160 170 180 RQADRYSVPR MVFVNKMDRT GADFLKVYGQ IKDRLKANAA PIQLPIGAEG DLSGIIDLVA 190 200 210 220 230 240 NKAYIYKNDL GTDIEESDIP ADMASEAAEW RAKLMETVAE TDEELIEQFL ENGELTEQQL 250 260 270 280 290 300 KKGIREGVLK HGLVPLLCGS AFKNKGVQLV LDAVVDYLPA PVDVPPIQGV LPNGEEAVRP 310 320 330 340 350 360 SDDSEPFSAL AFKVMADPYG KLTFVRMYSG VLEKGSYVTN STKDIKERIS RLVVLKADDR 370 380 390 400 410 420 EEVDQLRAGD LGAVLGLKNT TTGDTLCTTD EPIVLETLFI PEPVISVAVE PKTKGDMEKL 430 440 450 460 470 480 SKALVSLAEE DPTFRVSTDQ ETNQTVIAGM GELHLEILVD RMLREFKVEA NIGAPQVSYR 490 500 510 520 530 540 ETIRSSSKGE GKFARQTGGK GQYGHVVIEM EPGEPGTGFE FVNKIVGGVV PKEYIGPASN 550 560 570 580 590 600 GMKETCESGV LAGYPLIDVK VTMVDGSFHD VDSSEMAFKI AGSMAFKDGV KKCNPVLLEP 610 620 630 640 650 660 MMKVEVETPE DFLGSIIGDL SSRRGQVEGQ SIDDGQSKVQ AKVPLAEMFG YATQLRSMTQ 670 680 690 GRGIFSMEFS NYEEVPRNVA EAIISKNQGN S

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed: 18159947] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9211.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000878 Genomic DNA. Translation: ABX09563.1.
RefSeq	YP_001551517.1. NC_009976.1.
3D structure databases
ProteinModelPortal	A9BCK1.
SMR	A9BCK1. Positions 5-684.
ModBase	Search...
Protein-protein interaction databases
STRING	A9BCK1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5730094.
GenomeReviews	Gene locus P9211_16321 in contig CP000878_GR.
KEGG	pmj:P9211_16321.
PATRIC	22988938. VBIProMar136502_1734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG737692.
OMA	QGRAVYS.
PhylomeDB	A9BCK1.
ProtClustDB	PRK00007.
Family and domain databases
HAMAP	MF_00054_B. EF-G_EF-2_B. [Tree]
InterPro	IPR009022. Elongation_fac_G/III/V. IPR000795. ProtSyn_GTP-bd. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR005225. Small_GTP-bd_dom. IPR004540. Transl_elong_EFG/EF2. IPR000640. Transl_elong_EFG/EF2_C. IPR005517. Transl_elong_EFG/EF2_IV. IPR004161. Transl_elong_EFTu/EF1A_2. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.30.70.240. Transl_elong_EFG/EF2_C. 1 hit.
KO	K02355.
Pfam	PF00679. EFG_C. 1 hit. PF03764. EFG_IV. 1 hit. PF00009. GTP_EFTU. 1 hit. PF03144. GTP_EFTU_D2. 1 hit. [Graphical view]
PRINTS	PR00315. ELONGATNFCT.
SMART	SM00838. EFG_C. 1 hit. SM00889. EFG_IV. 1 hit. [Graphical view]
SUPFAM	SSF54980. EFG_III_V. 2 hits. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR00484. EF-G. 1 hit. TIGR00231. Small_GTP. 1 hit.
PROSITE	PS00301. EFACTOR_GTP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EFG_PROM4

Accession

Primary (citable) accession number: A9BCK1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program