A9BAY7 (GLGB_PROM4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	P9211_10681

Organism

Prochlorococcus marinus (strain MIT 9211) [Complete proteome] [HAMAP]

Taxonomic identifier

93059 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Cyanobacteria › Prochlorales › Prochlorococcaceae › Prochlorococcus › Prochlorococcus marinus

Protein attributes

Sequence length	759 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-HAMAP
Molecular_function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: UniProtKB-HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 759	759	1,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685		PRO_1000147760
Sites
	Active site	431	1	Nucleophile By similarity
	Active site	484	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9BAY7 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 6DD5F46C9793B497	FASTA	759	87,953
	10 20 30 40 50 60 MTTSFVSDWM AQEGQKLSDC KHDDPFSVLG IQPFQGKWIA RIWMPEAEEV ELLINGSLIP 70 80 90 100 110 120 LTPPNHPWIF EALLDENPGS NYQVKVHRGG IKHQQYDPWS FRDEWMGEID THLFAEGNHH 130 140 150 160 170 180 HIWRRMGAHL SEMNGVCGVM FCLWAPNARS VSVIGEVNSW DGRHHPMQKR MGGIWELFIP 190 200 210 220 230 240 GLKEGALYKY EIRTQNGHCY EKSDPYGFEH EVRPAQSSKV SRIDNFKWSD QEWITKRDSS 250 260 270 280 290 300 NPLDQPISVY EMHLGSWLHA PSDEPFIEPN GNKRTAVPAA DLKPGTRLLT YPELKQKLIK 310 320 330 340 350 360 YVKERGFTHI ELMPISEHPF DGSWGYQATG WYAPTSRYGT PNEFRAFVDA CHSEGIGVIL 370 380 390 400 410 420 DWVPGHFPKD SHGLAFFDGS HLYEHSDPRI GEHKEWGTLI FNYSRNEVRN FLVANLVFWF 430 440 450 460 470 480 EQFHIDGIRV DAVASMLYKD YLRPEGQWIP NEDGGNENTE AVKFLQQANH VLFEHFPGAL 490 500 510 520 530 540 SIAEESTTWP GVTNPTDVGG LGFNLKWNMG WMHDMLDYFE VDPWFRQFHQ NNVTFSITYN 550 560 570 580 590 600 YTENFMLALS HDEVVHGKSH LLHKMPGDDW RKYANTRALL AYMWTHPGKK TIFMGMEFGQ 610 620 630 640 650 660 RKEWNVWDDL EWDLLGYQPH QGIQRLVDDL NVLYKSNPAL WRDDFNQYGF QWIDCKDNKN 670 680 690 700 710 720 SVISFMRRET LNNEWLIVVA NFTPESHPNY RVGVPLEGFY EEIFNTDSDK YGGSNTGNMG 730 740 750 GKASEKWSIH EYEDSIDLSL PPLSVLVFKY AQKKSPNEN

References

[1]

"Patterns and implications of gene gain and loss in the evolution of Prochlorococcus." Kettler G.C., Martiny A.C., Huang K., Zucker J., Coleman M.L., Rodrigue S., Chen F., Lapidus A., Ferriera S., Johnson J., Steglich C., Church G.M., Richardson P., Chisholm S.W. PLoS Genet. 3:2515-2528(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MIT 9211.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000878 Genomic DNA. Translation: ABX08999.1.
RefSeq	YP_001550953.1. NC_009976.1.
3D structure databases
ProteinModelPortal	A9BAY7.
ModBase	Search...
MobiDB	Search...
Protein-protein interaction databases
STRING	93059.P9211_10681.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX08999; ABX08999; P9211_10681.
GeneID	5731278.
KEGG	pmj:P9211_10681.
PATRIC	22987706. VBIProMar136502_1129.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0296.
HOGENOM	HOG000283037.
KO	K00700.
OMA	KWIARIW.
OrthoDB	EOG6JX7GT.
Enzyme and pathway databases
BioCyc	PMAR93059:GHJV-1094-MONOMER.
UniPathway	UPA00164.
Family and domain databases
Gene3D	2.60.40.10. 2 hits. 2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
HAMAP	MF_00685. GlgB.
InterPro	IPR006048. A-amylase_b_C. IPR006407. GlgB. IPR015902. Glyco_hydro_13. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
PANTHER	PTHR10357. PTHR10357. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. SSF51445. 1 hit. SSF81296. SSF81296. 2 hits.
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_PROM4

Accession

Primary (citable) accession number: A9BAY7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	January 15, 2008
Last modified:	June 11, 2014
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program