ID   FTHS_HERA2              Reviewed;         570 AA.
AC   A9B4H8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   27-MAR-2024, entry version 82.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Haur_1499;
OS   Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785 / 114-95).
OC   Bacteria; Chloroflexota; Chloroflexia; Herpetosiphonales;
OC   Herpetosiphonaceae; Herpetosiphon.
OX   NCBI_TaxID=316274;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 23779 / DSM 785 / 114-95;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E.,
RA   Bryant D.A., Richardson P.;
RT   "Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000875; ABX04143.1; -; Genomic_DNA.
DR   AlphaFoldDB; A9B4H8; -.
DR   SMR; A9B4H8; -.
DR   STRING; 316274.Haur_1499; -.
DR   KEGG; hau:Haur_1499; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_0; -.
DR   InParanoid; A9B4H8; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000000787; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR020628; Formate_THF_ligase_CS.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR   PROSITE; PS00721; FTHFS_1; 1.
DR   PROSITE; PS00722; FTHFS_2; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism.
FT   CHAIN           1..570
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000146685"
FT   BINDING         65..72
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   570 AA;  60778 MW;  98957634815C5115 CRC64;
     MKTSLQIAAE ATPRPITQIA EELAIAEQFV EPYGRYRAKI NLDLLDASHD RPRGKQILVT
     AMTPTPLGEG KTATTIGLGM ALSRLGKRAI CTLRQSSLGP VFGIKGGGSG GGYSQVIPLE
     DSLMHLTGDI HAVTQAHNQI AAMTDNSWYQ KNRLGIDPEQ IQIRRVLDVN DRFLRSITIG
     QGGSQHGIPR QTGFDITAAS ELMAILALVS GENHADVMRD LRQRIGRMVV AFTRQGQPIT
     ADDIQAAGAA TVIMRNAIHP TLMQTIENTP VLMHGGPFAN IAHGNASVVA DQVGLRIADY
     VVTEAGFAMD MGGEKFFDIK CRAFDAKPAV VVLVATIRAL KAHSGRWNIK PGRDLPTDLL
     QENPDAVYAG GANLQKHIRN AQLFGLPVVV ALNSFPDDHP SEIEAVREIA MSAGAFDVAV
     SKVFSQGGVG GEELAEKVLA AIDQAGQAQF LYELEQPLTA KIATIATKIY GAAEVSYSEA
     ASEQLAKLEA NGFGNLPICM AKTHLSISHD PALKGAPTGY SFPIREVRAS IGAGFIYPIA
     GDMMTMPGLS ANPAAQQIDI DEHGNTVGLF
//