A9AYM5 (SYN_HERA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	Haur_2363

Organism

Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785) [Complete proteome] [HAMAP]

Taxonomic identifier

316274 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	447 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm By similarity HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	Asparagine--tRNA ligase HAMAP MF_00534		PRO_1000128208

Sequences

	Sequence		Length	Mass (Da)
	A9AYM5 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 076AFAEBC17B2296	FASTA	447	50,948
	10 20 30 40 50 60 MALLPSMYIR DSADHVGEAV KLAGWVYHKT EKGKLVFIQL RDGTATIQCV VFKKNVSEEV 70 80 90 100 110 120 FARAKELTQE SSCYIHGTLR ADERSSLGFE LDVTDIEIVH LTQNYPITPK EHGTQFLMEH 130 140 150 160 170 180 RHLWVRSAKQ HALLRIRAQV IAAAQEYLNS EHFVRYDSPI LTATAAEGTS DLFATEYFDL 190 200 210 220 230 240 GNAYLAQTGQ LYVESGMATF GRVYCFGPTF RAEKSKTRRH LTEFWMIEPE FAFADQDDNM 250 260 270 280 290 300 ELQENFVSYI VQRVLDRCEE DLKILERDTS KLENIVPPFP RISYDEAIEK IKQGVAAGAT 310 320 330 340 350 360 VAPDNAPLAD LEWGDDFGAP HETYLASLFD KPLFIYNYPT KVKAFYMQPA EGRPEVVRCA 370 380 390 400 410 420 DLIAPEGYGE IIGGSQRIHD AELLEARIRE HGLDVADYQW YLDLRRYGSV PHSGFGMGIE 430 440 RCVAWLAGTR HIREAIPFPR QLYRIYP

References

[1]

"Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Bryant D.A., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23779 / DSM 785.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000875 Genomic DNA. Translation: ABX05003.1.
RefSeq	YP_001545131.1. NC_009972.1.
3D structure databases
ProteinModelPortal	A9AYM5.
ModBase	Search...
Protein-protein interaction databases
STRING	A9AYM5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5734244.
GenomeReviews	Gene locus Haur_2363 in contig CP000875_GR.
KEGG	hau:Haur_2363.
PATRIC	22121791. VBIHerAur93466_2424.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG745843.
OMA	AIHRFFH.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	HAUR316274:HAUR_2363-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_HERA2

Accession

Primary (citable) accession number: A9AYM5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program