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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei258 – 2581ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciHAUR316274:GHYA-769-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Haur_0757
OrganismiHerpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
Taxonomic identifieri316274 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon
ProteomesiUP000000787 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 495495Glutamate--tRNA ligasePRO_1000090080Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi316274.Haur_0757.

Structurei

3D structure databases

ProteinModelPortaliA9AXJ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi14 – 2411"HIGH" regionAdd
BLAST
Motifi255 – 2595"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiMSKRHGS.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.

Sequencei

Sequence statusi: Complete.

A9AXJ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDRPTPART RFAPSPTGYL HIGSLRTVLF SWLWARHTGG QFLLRIEDTD
60 70 80 90 100
RKRFVEGAEE QLTSSLQAIG LMWDEGPIVG GPHAPYKQSE RLEIYQAHAQ
110 120 130 140 150
ALIDKGVAYR SYATADEIAA INAEREARGE PKLLVFRNLP GIDDAAREAA
160 170 180 190 200
GADYNVRLSL KTTGQTVVQD LVRGQIVFDN AALKMPDPVL LKTDGFPTYA
210 220 230 240 250
LAAMVDDHLM GITHVLRADE WIPTWPIHHQ IYEAFGWEQP VWVHVPQVLG
260 270 280 290 300
SDGKKLSKRH GDTSVTEYID LGFVPEAIIN YLALIGWSYD DKTEFMTLEE
310 320 330 340 350
LIERFDLNRI RPSGGVFDRD KLLHFNGVYL RNMAPAELAQ RVAPYLSKAG
360 370 380 390 400
LISAEPTAAE LAKITEYLPL VQDRLKLLSE APELLDFFFV DPQGYDPALL
410 420 430 440 450
VPKKGDPAQT VEILGQVKAS FEAVETWDAP SLDKLLHDFV NQLGLKIPQV
460 470 480 490
FMPIRVAISG RTTSPGLFET LAVLGKAVTL ARISTAAAAL SSASV
Length:495
Mass (Da):54,778
Last modified:January 15, 2008 - v1
Checksum:iA7916F5D4F4C7734
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000875 Genomic DNA. Translation: ABX03405.1.
RefSeqiYP_001543533.1. NC_009972.1.

Genome annotation databases

EnsemblBacteriaiABX03405; ABX03405; Haur_0757.
KEGGihau:Haur_0757.
PATRICi22118459. VBIHerAur93466_0775.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000875 Genomic DNA. Translation: ABX03405.1.
RefSeqiYP_001543533.1. NC_009972.1.

3D structure databases

ProteinModelPortaliA9AXJ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316274.Haur_0757.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX03405; ABX03405; Haur_0757.
KEGGihau:Haur_0757.
PATRICi22118459. VBIHerAur93466_0775.

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252720.
KOiK01885.
OMAiMSKRHGS.
OrthoDBiEOG6DRPF7.

Enzyme and pathway databases

BioCyciHAUR316274:GHYA-769-MONOMER.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
    , Kim E., Bryant D.A., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23779 / DSM 785.

Entry informationi

Entry nameiSYE_HERA2
AccessioniPrimary (citable) accession number: A9AXJ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: April 29, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.