A9AXJ1 (SYE_HERA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Haur_0757

Organism

Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785) [Complete proteome] [HAMAP]

Taxonomic identifier

316274 [NCBI]

Taxonomic lineage

Bacteria › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon

Protein attributes

Sequence length	495 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 495	495	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_1000090080
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_00022_B
	Motif	255 – 259	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	258	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AXJ1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: A7916F5D4F4C7734	FASTA	495	54,778
	10 20 30 40 50 60 MSDRPTPART RFAPSPTGYL HIGSLRTVLF SWLWARHTGG QFLLRIEDTD RKRFVEGAEE 70 80 90 100 110 120 QLTSSLQAIG LMWDEGPIVG GPHAPYKQSE RLEIYQAHAQ ALIDKGVAYR SYATADEIAA 130 140 150 160 170 180 INAEREARGE PKLLVFRNLP GIDDAAREAA GADYNVRLSL KTTGQTVVQD LVRGQIVFDN 190 200 210 220 230 240 AALKMPDPVL LKTDGFPTYA LAAMVDDHLM GITHVLRADE WIPTWPIHHQ IYEAFGWEQP 250 260 270 280 290 300 VWVHVPQVLG SDGKKLSKRH GDTSVTEYID LGFVPEAIIN YLALIGWSYD DKTEFMTLEE 310 320 330 340 350 360 LIERFDLNRI RPSGGVFDRD KLLHFNGVYL RNMAPAELAQ RVAPYLSKAG LISAEPTAAE 370 380 390 400 410 420 LAKITEYLPL VQDRLKLLSE APELLDFFFV DPQGYDPALL VPKKGDPAQT VEILGQVKAS 430 440 450 460 470 480 FEAVETWDAP SLDKLLHDFV NQLGLKIPQV FMPIRVAISG RTTSPGLFET LAVLGKAVTL 490 ARISTAAAAL SSASV

References

[1]

"Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Bryant D.A., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23779 / DSM 785.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000875 Genomic DNA. Translation: ABX03405.1.
RefSeq	YP_001543533.1. NC_009972.1.
3D structure databases
ProteinModelPortal	A9AXJ1.
ModBase	Search...
Protein-protein interaction databases
STRING	A9AXJ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5732641.
GenomeReviews	Gene locus Haur_0757 in contig CP000875_GR.
KEGG	hau:Haur_0757.
PATRIC	22118459. VBIHerAur93466_0775.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG628189.
OMA	MAHIPLI.
Enzyme and pathway databases
BioCyc	HAUR316274:HAUR_0757-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_HERA2

Accession

Primary (citable) accession number: A9AXJ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program