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Protein

Queuine tRNA-ribosyltransferase

Gene

tgt

Organism
Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Exchanges the guanine residue with 7-aminomethyl-7-deazaguanine in tRNAs with GUN anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).UniRule annotation

Catalytic activityi

Guanine(34) in tRNA + queuine = queuosine(34) in tRNA + guanine.UniRule annotation
Guanine(34) in tRNA + 7-aminomethyl-7-carbaguanine = 7-aminomethyl-7-carbaguanine(34) in tRNA + guanine.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei92 – 921NucleophileUniRule annotation
Binding sitei93 – 931SubstrateUniRule annotation
Metal bindingi303 – 3031ZincUniRule annotation
Metal bindingi305 – 3051ZincUniRule annotation
Metal bindingi308 – 3081ZincUniRule annotation
Metal bindingi334 – 3341ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Queuosine biosynthesis, tRNA processing

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciHAUR316274:GHYA-641-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
Queuine tRNA-ribosyltransferaseUniRule annotation (EC:2.4.2.29UniRule annotation)
Alternative name(s):
Guanine insertion enzymeUniRule annotation
tRNA-guanine transglycosylaseUniRule annotation
Gene namesi
Name:tgtUniRule annotation
Ordered Locus Names:Haur_0632
OrganismiHerpetosiphon aurantiacus (strain ATCC 23779 / DSM 785)
Taxonomic identifieri316274 [NCBI]
Taxonomic lineageiBacteriaChloroflexiChloroflexiaHerpetosiphonalesHerpetosiphonaceaeHerpetosiphon
Proteomesi
  • UP000000787 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 392392Queuine tRNA-ribosyltransferasePRO_1000203656Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316274.Haur_0632.

Structurei

3D structure databases

ProteinModelPortaliA9AW42.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the queuine tRNA-ribosyltransferase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.

Sequencei

Sequence statusi: Complete.

A9AW42-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNNFTYTLEH SDGEARAGQF STPHGTIQTP VFMPVGTQAT VKTLDPLEVE
60 70 80 90 100
AIGSQIILSN TYHLYLRPSA DLVAEMGGLH RFMQWPKPIL TDSGGFQVFS
110 120 130 140 150
LGPHSKIDED GVTFKSHIDG SKHRFTPESA IGIQEKLGAD IIMAFDECAP
160 170 180 190 200
QPTTHAYTKA AMERTHRWLL RCIAAKTRAD QALFGIVQGG VEADLRRESA
210 220 230 240 250
SFIAQQDVPG IGIGGLSVGE PKEQMYGMLE ETTPLLPRNK PRYLMGVGSP
260 270 280 290 300
EDLLEGVARG VDMFDCVLPT RLGRNGALFI PEGRLNIGNA KYAREDAPID
310 320 330 340 350
ATCDCSTCQR FSRAYLRHLF RTEEVLGLRL ATLHNLRFLI RLMEQAREAI
360 370 380 390
LQDRYQSFMD DWLSRFQTIP HAVREASRAA RLNSLRTQGD KA
Length:392
Mass (Da):43,590
Last modified:January 15, 2008 - v1
Checksum:i4F72293D37EFA667
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000875 Genomic DNA. Translation: ABX03280.1.

Genome annotation databases

EnsemblBacteriaiABX03280; ABX03280; Haur_0632.
KEGGihau:Haur_0632.
PATRICi22118197. VBIHerAur93466_0647.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000875 Genomic DNA. Translation: ABX03280.1.

3D structure databases

ProteinModelPortaliA9AW42.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316274.Haur_0632.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX03280; ABX03280; Haur_0632.
KEGGihau:Haur_0632.
PATRICi22118197. VBIHerAur93466_0647.

Phylogenomic databases

eggNOGiENOG4105C6U. Bacteria.
COG0343. LUCA.
HOGENOMiHOG000223473.
KOiK00773.
OMAiTPRFMPV.
OrthoDBiEOG6SNDVG.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciHAUR316274:GHYA-641-MONOMER.

Family and domain databases

Gene3Di3.20.20.105. 1 hit.
HAMAPiMF_00168. Q_tRNA_Tgt.
InterProiIPR004803. Queuine_tRNA-ribosylTrfase.
IPR002616. tRNA_ribo_trans-like.
[Graphical view]
PfamiPF01702. TGT. 1 hit.
[Graphical view]
SUPFAMiSSF51713. SSF51713. 1 hit.
TIGRFAMsiTIGR00430. Q_tRNA_tgt. 1 hit.
TIGR00449. tgt_general. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N.
    , Kim E., Bryant D.A., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23779 / DSM 785.

Entry informationi

Entry nameiTGT_HERA2
AccessioniPrimary (citable) accession number: A9AW42
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: January 15, 2008
Last modified: December 9, 2015
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.