A9AUP1 (PIMT_HERA2) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein-L-isoaspartate O-methyltransferase
EC=2.1.1.77

Alternative name(s):
L-isoaspartyl protein carboxyl methyltransferase
Protein L-isoaspartyl methyltransferase
Protein-beta-aspartate methyltransferase
Short name=PIMT

Gene names

Name:	pcm
Ordered Locus Names:	Haur_1925

Organism

Herpetosiphon aurantiacus (strain ATCC 23779 / DSM 785) [Complete proteome] [HAMAP]

Taxonomic identifier

316274 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Chloroflexi › Chloroflexi › Herpetosiphonales › Herpetosiphonaceae › Herpetosiphon › Herpetosiphon aurantiacus

Protein attributes

Sequence length	224 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins By similarity. HAMAP-Rule MF_00090
Catalytic activity	S-adenosyl-L-methionine + protein L-isoaspartate = S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester. HAMAP-Rule MF_00090
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00090.
Sequence similarities	Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein repair Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 224	224	Protein-L-isoaspartate O-methyltransferase HAMAP-Rule MF_00090		PRO_0000351868
Sites
	Active site	63	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AUP1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: E01B6FBCD033E34D	FASTA	224	24,685
	10 20 30 40 50 60 MSDVWQQQRQ RMVDEQLRPR GIHDQRILAA MANVPRHLFV PEALQAQAYS DQALPLTLGQ 70 80 90 100 110 120 TISQPYIVAL MAQELLLNPH EQLLEIGAGS GYAAAVFAEL VRKVVTIERH QALAQQTQVR 130 140 150 160 170 180 LRNLGYVNIE VVWGDGSLGY PTAAPYHAIS IPAATPQLAQ TLLSQLHDGG RLVAPIGDAQ 190 200 210 220 DQQLIRLQRQ GQNWQKTTIS NVRFVPLIGA GGWEHAPETT AEGE

References

[1]

"Complete sequence of chromosome of Herpetosiphon aurantiacus ATCC 23779." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. , Kim E., Bryant D.A., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23779 / DSM 785.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000875 Genomic DNA. Translation: ABX04568.1.
RefSeq	YP_001544696.1. NC_009972.1.
3D structure databases
ProteinModelPortal	A9AUP1.
ModBase	Search...
Protein-protein interaction databases
STRING	316274.Haur_1925.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX04568; ABX04568; Haur_1925.
GeneID	5733814.
KEGG	hau:Haur_1925.
PATRIC	22120901. VBIHerAur93466_1979.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2518.
HOGENOM	HOG000257189.
KO	K00573.
OMA	RILFSAC.
Enzyme and pathway databases
BioCyc	HAUR316274:GHYA-1954-MONOMER.
Family and domain databases
HAMAP	MF_00090. PIMT.
InterPro	IPR000682. PCMT. [Graphical view]
PANTHER	PTHR11579. PTHR11579. 1 hit.
Pfam	PF01135. PCMT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00080. pimt. 1 hit.
PROSITE	PS01279. PCMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PIMT_HERA2

Accession

Primary (citable) accession number: A9AUP1

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	January 15, 2008
Last modified:	May 29, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program