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A9ATE7 (A9ATE7_BURM1) Unreviewed, UniProtKB/TrEMBL

Last modified January 25, 2012. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Adenylosuccinate synthetase 2 HAMAP MF_00011
Short name=AMPSase 2 HAMAP MF_00011
Short name=AdSS 2 HAMAP MF_00011
EC=6.3.4.4 HAMAP MF_00011
Alternative name(s):
IMP--aspartate ligase 2 HAMAP MF_00011
Gene names
Name:purA EMBL BAG47202.1
Synonyms:purA2 HAMAP MF_00011
Ordered Locus Names:Bmul_5326, BMULJ_05365
OrganismBurkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]
Taxonomic identifier395019 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length432 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Plays an important role in the de novo pathway of purine nucleotide biosynthesis By similarity. RuleBase RU000520

Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP By similarity. HAMAP MF_00011

Catalytic activity

GTP + IMP + L-aspartate = GDP + phosphate + N(6)-(1,2-dicarboxyethyl)-AMP. RuleBase RU000520 HAMAP MF_00011

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00011

Pathway

Purine metabolism; AMP biosynthesis via de novo pathway; AMP from IMP: step 1/2. RuleBase RU000520 HAMAP MF_00011

Subunit structure

Homodimer By similarity. HAMAP MF_00011

Subcellular location

Cytoplasm By similarity HAMAP MF_00011.

Sequence similarities

Belongs to the adenylosuccinate synthetase family. RuleBase RU004163 HAMAP MF_00011

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Nucleotide binding12 – 187GTP By similarity HAMAP MF_00011
Nucleotide binding40 – 423GTP By similarity HAMAP MF_00011
Nucleotide binding329 – 3313GTP By similarity HAMAP MF_00011
Nucleotide binding411 – 4133GTP By similarity HAMAP MF_00011
Region13 – 164IMP binding By similarity HAMAP MF_00011
Region38 – 414IMP binding By similarity HAMAP MF_00011
Region297 – 3037Substrate binding By similarity HAMAP MF_00011

Sites

Active site131Proton acceptor By similarity HAMAP MF_00011
Active site411Proton donor By similarity HAMAP MF_00011
Metal binding131Magnesium By similarity HAMAP MF_00011
Metal binding401Magnesium; via carbonyl oxygen By similarity HAMAP MF_00011
Binding site1281IMP By similarity HAMAP MF_00011
Binding site1421IMP; shared with dimeric partner By similarity HAMAP MF_00011
Binding site2221IMP By similarity HAMAP MF_00011
Binding site2371IMP By similarity HAMAP MF_00011
Binding site3011IMP By similarity HAMAP MF_00011
Binding site3031GTP By similarity HAMAP MF_00011

Sequences

Sequence LengthMass (Da)Tools
A9ATE7 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: D8C056922365D76D

FASTA43246,339
        10         20         30         40         50         60 
MANVVVVGAQ WGDEGKGRIV DWLAARADVV ARYNGGHNAG HTLVVGGKTY KLALLPSGIV 

        70         80         90        100        110        120 
RGKRGVIGNG VVLDPEALLA EIGRMAELGL QVTPDNLSIA ENATLVLPIH RALDEAQERL 

       130        140        150        160        170        180 
RREPIGTTLR GIGPAYEDKV GRRGLRVGDL AEPGRLADKL EALVDYHNAW FRGLGLEVCS 

       190        200        210        220        230        240 
RDAMFDRLMD IAPKILPFVR PVWADLNDAT DRGEHILFEG SQAVMLDIDW GTYPFVTSSA 

       250        260        270        280        290        300 
TVASAAAAGT GLGASKLGHV LGVTKAYATR VGGGPFLTEL TDATGEALRT RGQEFGVNTG 

       310        320        330        340        350        360 
RPRRCGWLDA AQLRQPVRVS GIDSLALTKL DVLDGFDSIE LCVGYELDGA RVEHLPASLE 

       370        380        390        400        410        420 
AQARARPVYE RFDGWQRSVK GVRERAALPH AAQAFIARVE AVAGVPVSMI TTGAERDDTI 

       430 
VLRDPFDPAP RV

« Hide

References

[1]"Complete genome sequence of Burkholderia multivorans ATCC 17616."
Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17616 EMBL BAG47202.1 and ATCC 17616 / 249.
[2]"Complete sequence of chromosome 3 of Burkholderia multivorans ATCC 17616."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17616 / 249.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000870 Genomic DNA. Translation: ABX18996.1.
AP009387 Genomic DNA. Translation: BAG47202.1.
RefSeqYP_001585288.1. NC_010087.1.
YP_001941192.1. NC_010801.1.

3D structure databases

ProteinModelPortalA9ATE7.
ModBaseSearch...

Protein-protein interaction databases

STRINGA9ATE7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID5770554.
6356240.
GenomeReviewsGene locus BMULJ_05365 in contig AP009387_GR.
Gene locus Bmul_5326 in contig CP000870_GR.
KEGGbmj:BMULJ_05365.
bmu:Bmul_5326.
PATRIC19159775. VBIBurMul203716_0004.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG658237.
OMAYDELMQV.
ProtClustDBCLSK941367.

Family and domain databases

HAMAPMF_00011. Adenylosucc_synth.
[Tree]
InterProIPR018220. Adenylosuccinate_synthase_AS.
IPR001114. Adenylosuccinate_synthetase.
[Graphical view]
KOK01939.
PANTHERPTHR11846. Asucc_synthtase. 1 hit.
PfamPF00709. Adenylsucc_synt. 1 hit.
[Graphical view]
SMARTSM00788. Adenylsucc_synt. 1 hit.
[Graphical view]
TIGRFAMsTIGR00184. PurA. 1 hit.
PROSITEPS01266. ADENYLOSUCCIN_SYN_1. 1 hit.
PS00513. ADENYLOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameA9ATE7_BURM1
AccessionPrimary (citable) accession number: A9ATE7
Entry history
Integrated into UniProtKB/TrEMBL: January 15, 2008
Last sequence update: January 15, 2008
Last modified: January 25, 2012
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
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