Reviewed, UniProtKB/Swiss-Prot A9AMZ9 (BETA_BURM1)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	Bmul_3536, BMULJ_04981

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	566 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor. HAMAP MF_00750
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1. HAMAP MF_00750
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 566	566	Choline dehydrogenase HAMAP MF_00750		PRO_1000133323
Regions
	Nucleotide binding	7 – 36	30	FAD Probable
Sites
	Active site	474	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AMZ9-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 1D3CFF0E9E050550	FASTA	566	62,958
	10 20 30 40 50 60 MTTREYDYII CGAGSAGNVL ATRLTEDPNV TVLLLEAGGP DYRFDFRTQM PAALAYPLQG 70 80 90 100 110 120 RRYNWAYETD PEPHMDNRRM ECGRGKGLGG SSLINGMCYI RGNALDYDNW ATHQGLENWT 130 140 150 160 170 180 YLDCLPYFKK AETRDIGPND YHGGDGPVSV TTSKPGVNPL FEAMVEAGVQ AGYPRTEDLN 190 200 210 220 230 240 GYQQEGFGPM DRTVTPKGRR ASTARGYLDQ AKTRPNLEIV THALADRILF DGKRASGVTY 250 260 270 280 290 300 LRGNERATAH ARREVLVCSG AIASPQLLQR SGVGPGAWLK ELDIPIVLDL PGVGQNLQDH 310 320 330 340 350 360 LEMYIQYECK EPVSLYPALK WWNQPKIGLE WMLNGTGLGA SNHFEAGGFI RTRDDDPWPN 370 380 390 400 410 420 IQYHFLPVAI NYNGSNAIEM HGFQAHVGSM RSPSRGRVKL RSRDPNAHPS ILFNYMAEAL 430 440 450 460 470 480 DWREFRDAIR ATREIMRQPA LDRYRGRELN PGADLKSDKE LDAFVRARAE TAFHPSCSCK 490 500 510 520 530 540 MGYDDMAVVD NEGRVHGLEG LRVVDASIMP IITTGNLNAP TIMIAEKIAD KIRGRKPLER 550 560 ANVPYFVANG APARNVAKAV RQPETV

References

[1]

"Complete sequence of chromosome 2 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000869 Genomic DNA. Translation: ABX17220.1. AP009386 Genomic DNA. Translation: BAG46827.1.
RefSeq	YP_001583512.1. YP_001949363.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5769978. 6361992.
GenomeReviews	Gene locus BMULJ_04981 in contig AP009386_GR. Gene locus Bmul_3536 in contig CP000869_GR.
KEGG	bmj:BMULJ_04981. bmu:Bmul_3536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	PNLQYHF.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_BURM1

Accession

Primary (citable) accession number: A9AMZ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)