A9AKW7 (A9AKW7_BURM1) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Beta-galactosidase PIRNR PIRNR001084
Short name=Beta-gal PIRNR PIRNR001084
EC=3.2.1.23 PIRNR PIRNR001084

Gene names

Name:	lacZ EMBL BAG47166.1
Ordered Locus Names:	Bmul_3197 EMBL ABX16881.1, BMULJ_05328 EMBL BAG47166.1

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP] EMBL ABX16881.1

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex › Burkholderia multivorans

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides. PIRNR PIRNR001084
Sequence similarities	Belongs to the glycosyl hydrolase 42 family. PIRNR PIRNR001084

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR001084 EMBL ABX16881.1 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	beta-galactosidase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	beta-galactosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	142	1	Proton donor By similarity PIRSR PIRSR001084-1
	Active site	313	1	Nucleophile By similarity PIRSR PIRSR001084-1
	Binding site	103	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	141	1	Substrate By similarity PIRSR PIRSR001084-2
	Binding site	321	1	Substrate By similarity PIRSR PIRSR001084-2

Sequences

	Sequence		Length	Mass (Da)
	A9AKW7 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: D9D654062A2E4658	FASTA	661	74,176
	10 20 30 40 50 60 MRVGVCYYPE HWPESMWADD ARRMKALGIA QVRIAEFAWS RIEPSPGEYD WGWLDRAVDV 70 80 90 100 110 120 LAAAGLDIVM CTPTATPPKW LIDRHPEILA IGADGRPRTF GSRRHYDFSS PVYYDASRQI 130 140 150 160 170 180 CTAVAERYGR HPAVRFWQTD NEMGCHQTVV SYSPAALARF REWLKARYGT IDALNRAWGT 190 200 210 220 230 240 VFWSMEYRDF DEIDAPVGTV TEAHPTHRLD YRRFASDEVV RYHRMQVDVI RAHSPGRPVA 250 260 270 280 290 300 HNFMQLFTDF DHYAVARDLD VATWDSYPLG ALEEHWFAPD VKARWLRTGH PDFASFNHDL 310 320 330 340 350 360 YRGMSKLPFW VMEQQPGPVN WAHWNPAPLP GMVRLWSWEA FAHGAGCVSY FRWRQAPFAQ 370 380 390 400 410 420 EQMHAGLHTP DDRLDEGGRE AERVAHEIAV VHGADAHADA AVHARVALVF DYEAKWLLDV 430 440 450 460 470 480 QPQGADFQYA RIAFEYYSAL RALGLDVDVI SAQAPLDGYR LVVVPPLPIV PDDFAARLAA 490 500 510 520 530 540 SGAHAVFGPR TGSKTVDLQI PPTLPPGALA SILPLRVWRV ESLRPNVTER VDGALVDGTP 550 560 570 580 590 600 FAGHARHWRD LVELRDAQRT VVRARFADDH PAWLSHGTLH YWAALFDDAT TAQLFASVAA 610 620 630 640 650 660 EAGLAPTPLA DGVRISRRGG LTYVFNYGDT VHTIDAVPPA AFVVGSADVE PRGVAVYRSP V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 EMBL BAG47166.1 and ATCC 17616 / 249.
[2]	"Complete sequence of chromosome 2 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.
[3]	"Complete sequence of chromosome2 of Burkholderia multivorans ATCC 17616." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: ATCC 17616 EMBL ABX16881.1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000869 Genomic DNA. Translation: ABX16881.1. AP009386 Genomic DNA. Translation: BAG47166.1.
RefSeq	YP_001583173.1. NC_010086.1. YP_001949702.1. NC_010805.1.
3D structure databases
SMR	A9AKW7. Positions 3-658.
ModBase	Search...
Protein-protein interaction databases
STRING	395019.Bmul_3197.
Protein family/group databases
CAZy	GH42. Glycoside Hydrolase Family 42.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX16881; ABX16881; Bmul_3197. BAG47166; BAG47166; BMULJ_05328.
GeneID	5768083. 6361892.
KEGG	bmj:BMULJ_05328. bmu:Bmul_3197.
PATRIC	19172845. VBIBurMul203716_6453.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1874.
HOGENOM	HOG000117811.
KO	K12308.
OMA	GRFITHN.
ProtClustDB	CLSK875629.
Enzyme and pathway databases
BioCyc	BMUL395019:GIYO-5324-MONOMER.
Family and domain databases
Gene3D	2.60.40.1180. 1 hit. 3.20.20.80. 1 hit.
InterPro	IPR013738. Beta_galactosidase_Trimer. IPR013780. Glyco_hydro_13_b. IPR003476. Glyco_hydro_42. IPR013529. Glyco_hydro_42_N. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF02449. Glyco_hydro_42. 1 hit. PF08532. Glyco_hydro_42M. 1 hit. [Graphical view]
PIRSF	PIRSF001084. B-galactosidase. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A9AKW7_BURM1

Accession

Primary (citable) accession number: A9AKW7

Entry history

Integrated into UniProtKB/TrEMBL:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)