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A9AJP0

- PNP_BURM1

UniProt

A9AJP0 - PNP_BURM1

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Protein
Polyribonucleotide nucleotidyltransferase
Gene
pnp, Bmul_1024, BMULJ_02240
Organism
Burkholderia multivorans (strain ATCC 17616 / 249)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi493 – 4931Magnesium By similarity
Metal bindingi499 – 4991Magnesium By similarity

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. RNA binding Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. RNA processing Source: InterPro
  2. mRNA catabolic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBMUL395019:GIYO-2238-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferase (EC:2.7.7.8)
Alternative name(s):
Polynucleotide phosphorylase
Short name:
PNPase
Gene namesi
Name:pnp
Ordered Locus Names:Bmul_1024, BMULJ_02240
OrganismiBurkholderia multivorans (strain ATCC 17616 / 249)
Taxonomic identifieri395019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000007064: Chromosome 1, UP000008815: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 715715Polyribonucleotide nucleotidyltransferaseUniRule annotation
PRO_1000147896Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi395019.Bmul_1024.

Structurei

3D structure databases

ProteinModelPortaliA9AJP0.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini560 – 61960KH
Add
BLAST
Domaini629 – 69769S1 motif
Add
BLAST

Sequence similaritiesi

Contains 1 KH domain.
Contains 1 S1 motif domain.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218326.
KOiK00962.
OMAiPRWDWVA.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9AJP0-1 [UniParc]FASTAAdd to Basket

« Hide

MSMFNKVVKE FQWGQHKVRL ETGEIARQAS GAVIVDVEDT VVLATVVGAK    50
SAKPGQDFFP LTVDYIEKTY SAGKIPGGFF RREGRPSEHE TLTSRLIDRP 100
LRPLFPEGFY NEVQVVIHVL SVNPEIPADI PALIGASAAL AVSGLPFNGP 150
VGAARVAYVN DQYVLNPTRE QLKSSRLDLV VAGTERAVLM VESEADQLPE 200
EVMLGAVVFG HEQMQTAIDA IHELVRDGGK PEWDWQPAPK DEALIARVTE 250
IAQPELLAAY QIRDKQARST KLKEVYAATS AKLEEDALAA GTVAADKATV 300
GNILFDLEAK IVRGQILNGE PRIDGRDTRT VRPIEIRTGV LPRTHGSALF 350
TRGETQALVV ATLGTKGDEQ IIDALEGEYR ERFMLHYNMP PFATGETGRV 400
GSPKRREIGH GRLAKRALVA CLPSAEEFGY SIRVVSEITE SNGSSSMASV 450
CGGCLALMDA GVPMKAHVAG IAMGLILEGN KFAVLTDILG DEDHLGDMDF 500
KVAGTAQGVT ALQMDIKIQG ITKEIMQVAL AQAKEGRMHI LGKMTEAVAG 550
ANTQLSEFAP RMITIKINPE KIRDVIGKGG SVIRALTEET GTTIDISDDG 600
VVTIASTSSE GMAEAKKRIE NITAEIEVGQ VYEGTVLKLL DFGAIVNLLP 650
GKDGLLHISE IVNERVKDIN DYLKEGQQVK VKVIQTDEKG RVRLSAKALL 700
NEAAAAAQSD TPPQQ 715
Length:715
Mass (Da):77,131
Last modified:January 15, 2008 - v1
Checksum:i68754EE7DF89DCA2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000868 Genomic DNA. Translation: ABX14715.1.
AP009385 Genomic DNA. Translation: BAG44135.1.
RefSeqiYP_001579212.1. NC_010084.1.
YP_001946671.1. NC_010804.1.

Genome annotation databases

EnsemblBacteriaiABX14715; ABX14715; Bmul_1024.
BAG44135; BAG44135; BMULJ_02240.
GeneIDi5765265.
6360013.
KEGGibmj:BMULJ_02240.
bmu:Bmul_1024.
PATRICi19166334. VBIBurMul203716_3222.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000868 Genomic DNA. Translation: ABX14715.1 .
AP009385 Genomic DNA. Translation: BAG44135.1 .
RefSeqi YP_001579212.1. NC_010084.1.
YP_001946671.1. NC_010804.1.

3D structure databases

ProteinModelPortali A9AJP0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395019.Bmul_1024.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX14715 ; ABX14715 ; Bmul_1024 .
BAG44135 ; BAG44135 ; BMULJ_02240 .
GeneIDi 5765265.
6360013.
KEGGi bmj:BMULJ_02240.
bmu:Bmul_1024.
PATRICi 19166334. VBIBurMul203716_3222.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218326.
KOi K00962.
OMAi PRWDWVA.
OrthoDBi EOG6WT8CC.

Enzyme and pathway databases

BioCyci BMUL395019:GIYO-2238-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. RNA-binding_domain_S1.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.
  2. "Complete genome sequence of Burkholderia multivorans ATCC 17616."
    Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.

Entry informationi

Entry nameiPNP_BURM1
AccessioniPrimary (citable) accession number: A9AJP0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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