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A9AHA9 (SYL_BURM1) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Bmul_2732, BMULJ_00505
OrganismBurkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]
Taxonomic identifier395019 [NCBI]
Taxonomic lineageBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex

Protein attributes

Sequence length864 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

regulation of translational fidelity

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 864864Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091299

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif624 – 6285"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6271ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A9AHA9 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 22F866E5CCBD9029

FASTA86495,873
        10         20         30         40         50         60 
MHERYVPADV EAAAQGDWRA ADAYKTQEDA QKPKFYCVSM LPYPSGKLHM GHVRNYTIND 

        70         80         90        100        110        120 
VMYRYLRMNG YNVLMPMGWD AFGMPAENAA MANGVPPAKW TYDNIDYMKG QMQSMGLAID 

       130        140        150        160        170        180 
WSREIATCKP DYYKWNQWLF LKMLEKGIAY KKTGTVNWDP VDQTVLANEQ VIDGRGWRSG 

       190        200        210        220        230        240 
ALVEKREIPM YYLRITQYAD ELLNDLDGLG WPERVKIMQQ NWIGKSFGVN FGFPYELDGE 

       250        260        270        280        290        300 
KQLLRVFTTR ADTIMGVTFC AIAAEHPLAT RLAQGKPELQ AFIDECKRGG VAEADMATME 

       310        320        330        340        350        360 
KKGVATGFSV THPLTGEPVE VWIGNYVLMS YGEGAVMGVP GHDERDFAFA KKYGLPIKQV 

       370        380        390        400        410        420 
IAAEGHTYSL DAWQEWYGDK DIAVCINSGK YDGLGYAAAV DAVAADLKAG GFGDKQVTWR 

       430        440        450        460        470        480 
LRDWGISRQR YWGTPIPIIH CPSCGDVPVP EKDLPVVLPE DLVPDGSGNP LAKSEAFLNC 

       490        500        510        520        530        540 
TCPKCGAAAK RETDTMDTFV DSSWYFSRYT APDAETMVDA RTDYWMPMDQ YIGGIEHAIL 

       550        560        570        580        590        600 
HLLYSRFWTK VMRDLGLVKF GEPAKNLLTQ GMVLNETYYR EDAAGKKTWY NPADVTVTHD 

       610        620        630        640        650        660 
DKGRPVGATL NADGQPVVLG GIEKMSKSKN NGVDPQVLID QYGADTARLF TMFAAPPEQQ 

       670        680        690        700        710        720 
LEWSGAGVEG ASRFLRRVWS FGYANREALA SRAGFDAAAL GDADKALRRE IYSVLKQADF 

       730        740        750        760        770        780 
DYQRLQYNTV VSAAMKMLNA IDGAKGATPG VLREAYGVLL RVLYPVVPHV TYELWKALGY 

       790        800        810        820        830        840 
ADEFGPLLDA PWPKVDEAAL EQAEIELVLQ VNGKVRGALK VAKDASREAI EAAALADDAF 

       850        860 
AKFGEGKPAK KIVVVPGRLV NIVV 

« Hide

References

[1]"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616."
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17616 / 249.
[2]"Complete genome sequence of Burkholderia multivorans ATCC 17616."
Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M.
Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 17616 / 249.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000868 Genomic DNA. Translation: ABX16416.1.
AP009385 Genomic DNA. Translation: BAG42472.1.
RefSeqYP_001580913.1. NC_010084.1.
YP_001945008.1. NC_010804.1.

3D structure databases

ProteinModelPortalA9AHA9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING395019.Bmul_2732.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX16416; ABX16416; Bmul_2732.
BAG42472; BAG42472; BMULJ_00505.
GeneID5765905.
6359247.
KEGGbmj:BMULJ_00505.
bmu:Bmul_2732.
PATRIC19162701. VBIBurMul203716_1450.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycBMUL395019:GIYO-505-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BURM1
AccessionPrimary (citable) accession number: A9AHA9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: February 19, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries