Reviewed, UniProtKB/Swiss-Prot A9AGW1 (KDSA_BURM1)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	Bmul_1162, BMULJ_02092

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_1000091802

Sequences

	Sequence		Length	Mass (Da)
	A9AGW1-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 1F71644FC9A94DBC	FASTA	284	30,877
	10 20 30 40 50 60 MKLCDFEVGL DKPFFLIAGT CVVESEQMTI DTAGRLKEIC AKLNIPFIYK SSYDKANRSS 70 80 90 100 110 120 GKSFRGLGMD EGLRILSEVK RQLGLPVLTD VHSIDEIEQV ASVVDVLQTP AFLCRQTDFI 130 140 150 160 170 180 HACARSGKPV NIKKGQFLAP HDMKNVIDKA RDAAREAGLS EDRFMACERG VSFGYNNLVS 190 200 210 220 230 240 DMRSLAIMRE TNAPVVFDAT HSVQLPGGQG TSSGGQREFV PVLARAAVAT GVAGLFMETH 250 260 270 280 PNPAEAKSDG PNAVPLNRMS ALLETLVTLD QAVKRGPFLE NDFN

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000868 Genomic DNA. Translation: ABX14852.1. AP009385 Genomic DNA. Translation: BAG43999.1.
RefSeq	YP_001579349.1. YP_001946535.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5766118. 6359237.
GenomeReviews	Gene locus BMULJ_02092 in contig AP009385_GR. Gene locus Bmul_1162 in contig CP000868_GR.
KEGG	bmj:BMULJ_02092. bmu:Bmul_1162.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	VYKTSFD.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BURM1

Accession

Primary (citable) accession number: A9AGW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 15 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)