Reviewed, UniProtKB/Swiss-Prot A9AGQ1 (HUTI_BURM1)

Names and origin

Protein names

Recommended name:
    Imidazolonepropionase
    EC=3.5.2.7
Alternative name(s):
    Imidazolone-5-propionate hydrolase

Gene names

Name:	hutI
Ordered Locus Names:	Bmul_1102, BMULJ_02154

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+. HAMAP MF_00372
Cofactor	Binds 1 zinc or iron ion per subunit By similarity. HAMAP MF_00372
Pathway	Amino-acid degradation; L-histidine degradation into L-glutamate; N-formimidoyl-L-glutamate from L-histidine: step 3/3. HAMAP MF_00372
Subcellular location	Cytoplasm Potential HAMAP MF_00372.
Sequence similarities	Belongs to the hutI family.

Ontologies

Keywords
Biological process	Histidine metabolism
Cellular component	Cytoplasm
Ligand	Iron Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidine catabolic process to glutamate and formamide Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	imidazolonepropionase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Imidazolonepropionase HAMAP MF_00372		PRO_1000121538
Sites
	Metal binding	68	1	Zinc or iron By similarity
	Metal binding	70	1	Zinc or iron By similarity
	Metal binding	238	1	Zinc or iron By similarity
	Metal binding	313	1	Zinc or iron By similarity
	Binding site	77	1	Substrate By similarity
	Binding site	90	1	Substrate By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	Substrate By similarity
	Binding site	241	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AGQ1-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: FD18B6747F9CD6DE	FASTA	407	44,073
	10 20 30 40 50 60 MKPTVWHHLR LCPHGHPDET IDDAAIAVDE TGAIVWLGAC AALPHGYAHW RREDLHGAWV 70 80 90 100 110 120 TPGLVDCHTH LVYGGTRADE FAQRLAGVSY EEIARQGGGI VSTVRATRAA DETALFVQAA 130 140 150 160 170 180 ARLQPLLAEG VTAIEIKSGY GLDLASERKM LRVARQLGER FPVSVYTTFL GAHALPPEYA 190 200 210 220 230 240 GRADAYIDEI CERMLPTLAD EGLIDAVDVF CERIGFSLAQ TERVFEAATR RGLPVKLHAE 250 260 270 280 290 300 QLSDAGGTAL AARYHALSAD HLEFLDEAGI EAMKAAGTVA VLLPGAYYFI RETQLPPIDL 310 320 330 340 350 360 LRRHGVPIAL ATDHNPGTSP LESLLLTMNL GCTLFRMTVP EVLQGVTRHA AAALGRADRH 370 380 390 400 GALEVGRQAD FAVWSVGSLA ELAYWIGRPL CEQVVRGGTT VFRRMIG

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000868 Genomic DNA. Translation: ABX14792.1. AP009385 Genomic DNA. Translation: BAG44058.1.
RefSeq	YP_001579289.1. YP_001946594.1.
3D structure databases
SMR	A9AGQ1. Positions 4-401.
ModBase	Search...
Genome annotation databases
GeneID	5766612. 6359539.
GenomeReviews	Gene locus BMULJ_02154 in contig AP009385_GR. Gene locus Bmul_1102 in contig CP000868_GR.
KEGG	bmj:BMULJ_02154. bmu:Bmul_1102.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG686142.
OMA	MNMACTL.
Family and domain databases
HAMAP	MF_00372. HutI. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR005920. HutI. IPR011059. Metal-dep_hydrolase_composite. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01224. hutI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HUTI_BURM1

Accession

Primary (citable) accession number: A9AGQ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)