Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

A9AGN6

- SYI_BURM1

UniProt

A9AGN6 - SYI_BURM1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia multivorans (strain ATCC 17616 / 249)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylateUniRule annotation
Binding sitei625 – 6251ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi928 – 9281ZincUniRule annotation
Metal bindingi931 – 9311ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBMUL395019:GIYO-2475-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bmul_0783, BMULJ_02477
OrganismiBurkholderia multivorans (strain ATCC 17616 / 249)
Taxonomic identifieri395019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000007064: Chromosome 1, UP000008815: Chromosome 1

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligasePRO_1000189138Add
BLAST

Proteomic databases

PRIDEiA9AGN6.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi395019.Bmul_0783.

Structurei

3D structure databases

ProteinModelPortaliA9AGN6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionAdd
BLAST
Motifi622 – 6265"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9AGN6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGVYEKIR
60 70 80 90 100
AASKGRPKFI LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMSK ARAYATEQIE KQKAGFKRLG
160 170 180 190 200
VLGDWADPYK TMNFVNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDR TDPTIDVMFP FAEPEKTAQA FGLPALPRAE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTERGLLIVA EERVAACMEE FKLSGRVVAT
310 320 330 340 350
APGVKLVNLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG
360 370 380 390 400
VEDFISCKTH GMTDSDIINP VMGDGRYIES LPLFGGLSIW DANPKVVDAL
410 420 430 440 450
RDAGTLLRSE KYTHSYMHCW RHKTPIIYRA TSQWFAGMDV TPREGGKTLR
460 470 480 490 500
ETALEGVEAT AFYPSWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAKRVE KSGIEAWQTL DPRELIGDDA NLYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG VDPHEVANRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAQHAVPVDE
710 720 730 740 750
WLEIDRYAVA FSAQLQTELL AYYEKYEFHP VVAKLQTYCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTSAP DSRARRSAQT ALYHLTQGLL RVLAPFLSFT AEEAWKVFQP
810 820 830 840 850
ASDTIFTETY YAYPDVADAD ALIDKWSLLR DVRGTVTKAL EEARTANRIG
860 870 880 890 900
SSLQAEVTVH ASGARYDALA SLGDDLKFVL ITSAATVVKV DDEAQEGVDV
910 920 930 940
AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE IRSAA
Length:945
Mass (Da):106,062
Last modified:January 15, 2008 - v1
Checksum:i895D622E8ADA8634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000868 Genomic DNA. Translation: ABX14478.1.
AP009385 Genomic DNA. Translation: BAG44368.1.
RefSeqiWP_006416148.1. NC_010804.1.
YP_001578975.1. NC_010084.1.
YP_001946904.1. NC_010804.1.

Genome annotation databases

EnsemblBacteriaiABX14478; ABX14478; Bmul_0783.
BAG44368; BAG44368; BMULJ_02477.
GeneIDi5765949.
6360053.
KEGGibmj:BMULJ_02477.
bmu:Bmul_0783.
PATRICi19166854. VBIBurMul203716_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000868 Genomic DNA. Translation: ABX14478.1 .
AP009385 Genomic DNA. Translation: BAG44368.1 .
RefSeqi WP_006416148.1. NC_010804.1.
YP_001578975.1. NC_010084.1.
YP_001946904.1. NC_010804.1.

3D structure databases

ProteinModelPortali A9AGN6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395019.Bmul_0783.

Proteomic databases

PRIDEi A9AGN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX14478 ; ABX14478 ; Bmul_0783 .
BAG44368 ; BAG44368 ; BMULJ_02477 .
GeneIDi 5765949.
6360053.
KEGGi bmj:BMULJ_02477.
bmu:Bmul_0783.
PATRICi 19166854. VBIBurMul203716_3480.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BMUL395019:GIYO-2475-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.
  2. "Complete genome sequence of Burkholderia multivorans ATCC 17616."
    Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.

Entry informationi

Entry nameiSYI_BURM1
AccessioniPrimary (citable) accession number: A9AGN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: November 26, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3