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A9AGN6

- SYI_BURM1

UniProt

A9AGN6 - SYI_BURM1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Bmul_0783, BMULJ_02477
Organism
Burkholderia multivorans (strain ATCC 17616 / 249)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei581 – 5811Aminoacyl-adenylate By similarity
Binding sitei625 – 6251ATP By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity
Metal bindingi928 – 9281Zinc By similarity
Metal bindingi931 – 9311Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBMUL395019:GIYO-2475-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Bmul_0783, BMULJ_02477
OrganismiBurkholderia multivorans (strain ATCC 17616 / 249)
Taxonomic identifieri395019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
ProteomesiUP000007064: Chromosome 1, UP000008815: Chromosome 1

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 945945Isoleucine--tRNA ligaseUniRule annotation
PRO_1000189138Add
BLAST

Proteomic databases

PRIDEiA9AGN6.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi395019.Bmul_0783.

Structurei

3D structure databases

ProteinModelPortaliA9AGN6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi66 – 7611"HIGH" regionUniRule annotation
Add
BLAST
Motifi622 – 6265"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9AGN6-1 [UniParc]FASTAAdd to Basket

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MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGVYEKIR    50
AASKGRPKFI LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV 100
PGWDCHGMPI EIQIEKQFGK SLPAAEVMSK ARAYATEQIE KQKAGFKRLG 150
VLGDWADPYK TMNFVNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL 200
AEAEVEYKDR TDPTIDVMFP FAEPEKTAQA FGLPALPRAE GGIVIWTTTP 250
WTIPANQALN LHPEIVYALV DTERGLLIVA EERVAACMEE FKLSGRVVAT 300
APGVKLVNLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG 350
VEDFISCKTH GMTDSDIINP VMGDGRYIES LPLFGGLSIW DANPKVVDAL 400
RDAGTLLRSE KYTHSYMHCW RHKTPIIYRA TSQWFAGMDV TPREGGKTLR 450
ETALEGVEAT AFYPSWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE 500
TGELHPRTLE LLEEVAKRVE KSGIEAWQTL DPRELIGDDA NLYEKNRDTL 550
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML 600
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG VDPHEVANRL GAEIIRLWIA 650
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAQHAVPVDE 700
WLEIDRYAVA FSAQLQTELL AYYEKYEFHP VVAKLQTYCS EDLGGFYLDV 750
LKDRLYTSAP DSRARRSAQT ALYHLTQGLL RVLAPFLSFT AEEAWKVFQP 800
ASDTIFTETY YAYPDVADAD ALIDKWSLLR DVRGTVTKAL EEARTANRIG 850
SSLQAEVTVH ASGARYDALA SLGDDLKFVL ITSAATVVKV DDEAQEGVDV 900
AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE IRSAA 945
Length:945
Mass (Da):106,062
Last modified:January 15, 2008 - v1
Checksum:i895D622E8ADA8634
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000868 Genomic DNA. Translation: ABX14478.1.
AP009385 Genomic DNA. Translation: BAG44368.1.
RefSeqiYP_001578975.1. NC_010084.1.
YP_001946904.1. NC_010804.1.

Genome annotation databases

EnsemblBacteriaiABX14478; ABX14478; Bmul_0783.
BAG44368; BAG44368; BMULJ_02477.
GeneIDi5765949.
6360053.
KEGGibmj:BMULJ_02477.
bmu:Bmul_0783.
PATRICi19166854. VBIBurMul203716_3480.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000868 Genomic DNA. Translation: ABX14478.1 .
AP009385 Genomic DNA. Translation: BAG44368.1 .
RefSeqi YP_001578975.1. NC_010084.1.
YP_001946904.1. NC_010804.1.

3D structure databases

ProteinModelPortali A9AGN6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 395019.Bmul_0783.

Proteomic databases

PRIDEi A9AGN6.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX14478 ; ABX14478 ; Bmul_0783 .
BAG44368 ; BAG44368 ; BMULJ_02477 .
GeneIDi 5765949.
6360053.
KEGGi bmj:BMULJ_02477.
bmu:Bmul_0783.
PATRICi 19166854. VBIBurMul203716_3480.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BMUL395019:GIYO-2475-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616."
    Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.
  2. "Complete genome sequence of Burkholderia multivorans ATCC 17616."
    Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M.
    Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 17616 / 249.

Entry informationi

Entry nameiSYI_BURM1
AccessioniPrimary (citable) accession number: A9AGN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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