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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Burkholderia multivorans (strain ATCC 17616 / 249)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei581Aminoacyl-adenylateUniRule annotation1
Binding sitei625ATPUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi928ZincUniRule annotation1
Metal bindingi931ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Bmul_0783, BMULJ_02477
OrganismiBurkholderia multivorans (strain ATCC 17616 / 249)
Taxonomic identifieri395019 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeBurkholderiaBurkholderia cepacia complex
Proteomesi
  • UP000008815 Componenti: Chromosome 1

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891381 – 945Isoleucine--tRNA ligaseAdd BLAST945

Proteomic databases

PRIDEiA9AGN6

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi395019.BMULJ_02477

Structurei

3D structure databases

ProteinModelPortaliA9AGN6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi66 – 76"HIGH" regionAdd BLAST11
Motifi622 – 626"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

A9AGN6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKKADSKP QAKYPVNLLD TPFPMRGDLP KREPQWVKEW EERGVYEKIR
60 70 80 90 100
AASKGRPKFI LHDGPPYANG DIHLGHAVNK ILKDIVVKSR NMAGFDAPYV
110 120 130 140 150
PGWDCHGMPI EIQIEKQFGK SLPAAEVMSK ARAYATEQIE KQKAGFKRLG
160 170 180 190 200
VLGDWADPYK TMNFVNEAEE IRALGKIIEK GYVYRGLKPV NWCFDCGSAL
210 220 230 240 250
AEAEVEYKDR TDPTIDVMFP FAEPEKTAQA FGLPALPRAE GGIVIWTTTP
260 270 280 290 300
WTIPANQALN LHPEIVYALV DTERGLLIVA EERVAACMEE FKLSGRVVAT
310 320 330 340 350
APGVKLVNLR FHHPLASAHP GYKRTAPVYL GDYVTTDTGT GVVHSSPAYG
360 370 380 390 400
VEDFISCKTH GMTDSDIINP VMGDGRYIES LPLFGGLSIW DANPKVVDAL
410 420 430 440 450
RDAGTLLRSE KYTHSYMHCW RHKTPIIYRA TSQWFAGMDV TPREGGKTLR
460 470 480 490 500
ETALEGVEAT AFYPSWGKQR LFSMIANRPD WTLSRQRQWG VPMAFFVHKE
510 520 530 540 550
TGELHPRTLE LLEEVAKRVE KSGIEAWQTL DPRELIGDDA NLYEKNRDTL
560 570 580 590 600
DVWFDSGTTH WHVLRGSHKD QLQFPADLYL EGSDQHRGWF HSSLLTASML
610 620 630 640 650
DGRAPYKGLL THGFTVDGEG RKMSKSLGNG VDPHEVANRL GAEIIRLWIA
660 670 680 690 700
STDYSGELAI SEEILKRVTE GYRRIRNTLR FLLANLSDFD YAQHAVPVDE
710 720 730 740 750
WLEIDRYAVA FSAQLQTELL AYYEKYEFHP VVAKLQTYCS EDLGGFYLDV
760 770 780 790 800
LKDRLYTSAP DSRARRSAQT ALYHLTQGLL RVLAPFLSFT AEEAWKVFQP
810 820 830 840 850
ASDTIFTETY YAYPDVADAD ALIDKWSLLR DVRGTVTKAL EEARTANRIG
860 870 880 890 900
SSLQAEVTVH ASGARYDALA SLGDDLKFVL ITSAATVVKV DDEAQEGVDV
910 920 930 940
AASKYQKCER CWHYREDVGA HADHPTLCGR CFSNLFENGE IRSAA
Length:945
Mass (Da):106,062
Last modified:January 15, 2008 - v1
Checksum:i895D622E8ADA8634
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000868 Genomic DNA Translation: ABX14478.1
AP009385 Genomic DNA Translation: BAG44368.1
RefSeqiWP_006416148.1, NC_010804.1

Genome annotation databases

EnsemblBacteriaiABX14478; ABX14478; Bmul_0783
BAG44368; BAG44368; BMULJ_02477
KEGGibmj:BMULJ_02477
bmu:Bmul_0783

Similar proteinsi

Entry informationi

Entry nameiSYI_BURM1
AccessioniPrimary (citable) accession number: A9AGN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: April 25, 2018
This is version 81 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health