Reviewed, UniProtKB/Swiss-Prot A9AGN3 (DUT_BURM1)

Names and origin

Protein names

Recommended name:
    Deoxyuridine 5'-triphosphate nucleotidohydrolase
      Short name=dUTPase
    EC=3.6.1.23
Alternative name(s):
    dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	Bmul_0780, BMULJ_02480

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	148 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity.
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity.
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 148	148	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000094947
Regions
	Region	67 – 69	3	Substrate binding By similarity
	Region	84 – 86	3	Substrate binding By similarity
Sites
	Binding site	80	1	Substrate By similarity
	Binding site	94	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AGN3-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: F67FB86AF77FF35B	FASTA	148	15,851
	10 20 30 40 50 60 MKLDLKILDA RMRDYLPAYA TAGSAGLDLR ACLDAPLTLK PGDTALVPTG LAIHLADPNY 70 80 90 100 110 120 AALILPRSGL GHKHGIVLGN LVGLIDSDYQ GQLMVSTWNR GQTEFVLNPF ERLAQLVIVP 130 140 VVQAQFNIVD DFAQSERGAG GFGSTGRH

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000868 Genomic DNA. Translation: ABX14475.1. AP009385 Genomic DNA. Translation: BAG44371.1.
RefSeq	YP_001578972.1. YP_001946907.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5767963. 6359187.
GenomeReviews	Gene locus BMULJ_02480 in contig AP009385_GR. Gene locus Bmul_0780 in contig CP000868_GR.
KEGG	bmj:BMULJ_02480. bmu:Bmul_0780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LDLRACI.
Family and domain databases
HAMAP	MF_00116. [Tree]
InterPro	IPR008180. DeoxyUTP_pyroPase_dom. IPR008181. dUTP_pyrophosphatase_subfam_1. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BURM1

Accession

Primary (citable) accession number: A9AGN3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)