A9AGH1 (T23O_BURM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan 2,3-dioxygenase
Short name=TDO
EC=1.13.11.11

Alternative name(s):
Tryptamin 2,3-dioxygenase
Tryptophan oxygenase
Short name=TO
Short name=TRPO
Tryptophan pyrrolase
Tryptophanase

Gene names

Name:	kynA
Ordered Locus Names:	Bmul_0718, BMULJ_02542

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the oxidative cleavage of the L-tryptophan (L-Trp) pyrrole ring By similarity.
Catalytic activity	L-tryptophan + O2 = N-formyl-L-kynurenine.
Cofactor	Binds 2 heme groups per tetramer By similarity.
Pathway	Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 1/2.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the tryptophan 2,3-dioxygenase family.
Sequence caution	The sequence ABX14413.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Tryptophan catabolism
Ligand	Heme Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein homotetramerization Inferred from sequence or structural similarity. Source: UniProtKB tryptophan catabolic process to kynurenine Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	heme binding Inferred from sequence or structural similarity. Source: UniProtKB tryptophan 2,3-dioxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Tryptophan 2,3-dioxygenase		PRO_0000360102
Regions
	Region	54 – 58	5	Substrate binding By similarity
	Region	79 – 83	5	Substrate binding By similarity
Sites
	Metal binding	268	1	Iron (heme axial ligand) By similarity
	Binding site	141	1	Substrate By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	152	1	Heme By similarity
	Binding site	282	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AGH1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 9064E432AAB811FB	FASTA	310	35,343
	10 20 30 40 50 60 MQPPGNDAPA GCPFSGARAQ GTQAAHEAPH VPGDAGEQAG WHNAQLDFSK SMSYGDYLSL 70 80 90 100 110 120 NAILNAQHPL SPDHNEMLFI IQHQTSELWM KLALFELRGA LDAVRSDALP PAFKMLARVS 130 140 150 160 170 180 RILEQLVQAW NVLSTMTPSE YSAMRPYLGQ SSGFQSYQYR QLEFLLGNKN AQMLQPHAHR 190 200 210 220 230 240 PDILEQVRAT LDAPSFYDEV VRLLARRGFP IAPSRLDRDW RQPTQHDETV EAAWLEVYRH 250 260 270 280 290 300 PQQHWELYEM AEELVDLEDA FRQWRFRHVT TVERIIGFKQ GTGGTSGAPY LRKMLDVVLF 310 PELWHVRTTL

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000868 Genomic DNA. Translation: ABX14413.1. Different initiation. AP009385 Genomic DNA. Translation: BAG44433.1.
RefSeq	YP_001578910.1. NC_010084.1. YP_001946969.1. NC_010804.1.
3D structure databases
ProteinModelPortal	A9AGH1.
ModBase	Search...
Protein-protein interaction databases
STRING	A9AGH1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5767643. 6359606.
GenomeReviews	Gene locus BMULJ_02542 in contig AP009385_GR. Gene locus Bmul_0718 in contig CP000868_GR.
KEGG	bmj:BMULJ_02542. bmu:Bmul_0718.
PATRIC	19166993. VBIBurMul203716_3549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG647485.
OMA	QYREIEF.
ProtClustDB	CLSK930429.
Family and domain databases
InterPro	IPR017485. Trp_2-3-dOase_bac. IPR004981. Trp_2_3_dOase. [Graphical view]
KO	K00453.
PANTHER	PTHR10138. Trp_2_3_dOase. 1 hit.
Pfam	PF03301. Trp_dioxygenase. 1 hit. [Graphical view]
TIGRFAMs	TIGR03036. Trp_2_3_diox. 1 hit.
ProtoNet	Search...

Entry information

Entry name

T23O_BURM1

Accession

Primary (citable) accession number: A9AGH1
Secondary accession number(s): B3D321

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 20, 2009
Last sequence update:	January 20, 2009
Last modified:	January 25, 2012
This is version 28 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program