A9AFB4 (QUEF_BURM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductase
EC=1.7.1.13

Alternative name(s):
7-cyano-7-carbaguanine reductase
NADPH-dependent nitrile oxidoreductase
PreQ(0) reductase

Gene names

Name:	queF
Ordered Locus Names:	Bmul_0561, BMULJ_02700

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) By similarity. HAMAP MF_00817
Catalytic activity	7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH. HAMAP MF_00817
Pathway	tRNA modification; tRNA-queuosine biosynthesis. HAMAP MF_00817
Subcellular location	Cytoplasm By similarity HAMAP MF_00817.
Sequence similarities	Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Ontologies

Keywords
Biological process	Queuosine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	queuosine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro preQ1 synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NADPH-dependent 7-cyano-7-deazaguanine reductase HAMAP MF_00817		PRO_1000134271

Sequences

	Sequence		Length	Mass (Da)
	A9AFB4 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: D708B2721F992AE4	FASTA	274	30,416
	10 20 30 40 50 60 MNPEHSPLGK ATVYASQYDA SLLFPIPRAG AREQIGITSA LPFFGTDIWN AYELSWLNAR 70 80 90 100 110 120 GKPQVAVATF YVPAESPNIV ESKSFKLYLG SFAQSKFDSV DAVRDVLKRD VSAACGASVS 130 140 150 160 170 180 VQLVSPHDFG KLEMDELDGL SLDRLDLDAD VYEPDPSLLS AAEDEAPVEE TLVSDLLKSN 190 200 210 220 230 240 CPVTGQPDWG SVQIHYVGPQ IDHAGLLRYI ISFRNHTGFH EQCVERIFLD ILHRCKPVKL 250 260 270 AVYARYTRRG GLDINPFRTN YNQPMPDNAR TARQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000868 Genomic DNA. Translation: ABX14256.1. AP009385 Genomic DNA. Translation: BAG44590.1.
RefSeq	YP_001578753.1. NC_010084.1. YP_001947126.1. NC_010804.1.
3D structure databases
ProteinModelPortal	A9AFB4.
ModBase	Search...
Protein-protein interaction databases
STRING	A9AFB4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5765536. 6359550.
GenomeReviews	Gene locus BMULJ_02700 in contig AP009385_GR. Gene locus Bmul_0561 in contig CP000868_GR.
KEGG	bmj:BMULJ_02700. bmu:Bmul_0561.
PATRIC	19167326. VBIBurMul203716_3714.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289139.
OMA	CVERIFC.
ProtClustDB	PRK11792.
Family and domain databases
HAMAP	MF_00817. QueF_type2. [Tree]
InterPro	IPR016428. CN_OxRdtase_NADPH-dep_YqcD. IPR020602. GTP_CycHdrlase_I/CN_OxRdtase. [Graphical view]
KO	K06879.
Pfam	PF01227. GTP_cyclohydroI. 1 hit. [Graphical view]
PIRSF	PIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMs	TIGR03138. QueF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

QUEF_BURM1

Accession

Primary (citable) accession number: A9AFB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program