A9AEN5 (F16PA_BURM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-1,6-bisphosphatase class 1
Short name=FBPase class 1
EC=3.1.3.11

Alternative name(s):
D-fructose-1,6-bisphosphate 1-phosphohydrolase class 1

Gene names

Name:	fbp
Ordered Locus Names:	Bmul_2381, BMULJ_00860

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate + H2O = D-fructose 6-phosphate + phosphate. HAMAP MF_01855
Cofactor	Binds 2 magnesium ions per subunit By similarity. HAMAP MF_01855
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_01855
Subunit structure	Homotetramer By similarity. HAMAP MF_01855
Subcellular location	Cytoplasm Potential HAMAP MF_01855.
Sequence similarities	Belongs to the FBPase class 1 family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fructose 1,6-bisphosphate 1-phosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Fructose-1,6-bisphosphatase class 1 HAMAP MF_01855		PRO_0000364494
Regions
	Region	119 – 122	4	Substrate binding By similarity
Sites
	Metal binding	94	1	Magnesium 1 By similarity
	Metal binding	116	1	Magnesium 1 By similarity
	Metal binding	116	1	Magnesium 2 By similarity
	Metal binding	118	1	Magnesium 1; via carbonyl oxygen By similarity
	Metal binding	119	1	Magnesium 2 By similarity
	Metal binding	282	1	Magnesium 2 By similarity
	Binding site	210	1	Substrate By similarity
	Binding site	276	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AEN5 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: D75094533DC9A283	FASTA	337	37,162
	10 20 30 40 50 60 MSIARRTTLS KFLIEQQRET NNLPADLRLL IEVVARACKA ISYNVSKGAL GDALGTAGSE 70 80 90 100 110 120 NVQGEVQKKL DILSNEILLD ANEWGGNLAA MASEEMETFF PIPANYPRGE YLLVFDPLDG 130 140 150 160 170 180 SSNIDVNVSI GTIFSVLRCP DGKQATEESF LQPGTQQVAA GYAVYGPQTV FVLTTGNGVN 190 200 210 220 230 240 CFTLDREVGS WVLTQSNMQI PADTREYAIN ASNARHWYDP VKRYVDELNA GKDGPRGDNF 250 260 270 280 290 300 NMRWIASMVA DVHRILNRGG IFMYPADKRT PDRPGKLRLM YEANPMAFIV EQAGGAATTG 310 320 330 TQRIMEVQPT GLHQRVPVFL GSKNEVERVT AYHHESQ

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000868 Genomic DNA. Translation: ABX16066.1. AP009385 Genomic DNA. Translation: BAG42813.1.
RefSeq	YP_001580563.1. NC_010084.1. YP_001945349.1. NC_010804.1.
3D structure databases
ProteinModelPortal	A9AEN5.
ModBase	Search...
Protein-protein interaction databases
STRING	A9AEN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5767444. 6357652.
GenomeReviews	Gene locus BMULJ_00860 in contig AP009385_GR. Gene locus Bmul_2381 in contig CP000868_GR.
KEGG	bmj:BMULJ_00860. bmu:Bmul_2381.
PATRIC	19163458. VBIBurMul203716_1820.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG731261.
OMA	HWEAPVQ.
ProtClustDB	PRK09293.
Family and domain databases
HAMAP	MF_01855. FBPase_class1. [Tree]
InterPro	IPR000146. FBPase_class-1/SBPase. [Graphical view]
KO	K03841.
PANTHER	PTHR11556. In_FB_phphtase. 1 hit.
Pfam	PF00316. FBPase. 1 hit. [Graphical view]
PIRSF	PIRSF000904. FBPtase_SBPase. 1 hit.
PRINTS	PR00115. F16BPHPHTASE.
PROSITE	PS00124. FBPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

F16PA_BURM1

Accession

Primary (citable) accession number: A9AEN5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program