A9ADV6 (AROA_BURM1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	Bmul_2259, BMULJ_00980

Organism

Burkholderia multivorans (strain ATCC 17616 / 249) [Complete proteome] [HAMAP]

Taxonomic identifier

395019 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Burkholderia › Burkholderia cepacia complex

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_1000099672

Sequences

	Sequence		Length	Mass (Da)
	A9ADV6 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: D3DFE9CA61FF7319	FASTA	434	46,302
	10 20 30 40 50 60 MDYLDLGPYS SASGTVRLPG SKSISNRVLL LAALAEGDTT ITNLLDSDDT RVMLDALGKL 70 80 90 100 110 120 GVKLARDGDT CVVTGTRGAF TAKTADLFLG NAGTAVRPLT AALAINGGDY RVHGVPRMHE 130 140 150 160 170 180 RPIGDLVDGL RQIGAQIDYE QNEGFPPLRI RPATISVDAP IRVRGDVSSQ FLTALLMTLP 190 200 210 220 230 240 LVKAKDGRSI VEIDGELISK PYIDITIRLM ARFGVNVERE GWQRFTVPAG VRYRSPGRIM 250 260 270 280 290 300 VEGDASSASY FLAAGALGGG PLRVEGVGRA SIQGDVGFAH ALMQMGANVT MGDDWIEVRG 310 320 330 340 350 360 IGHDHGKLEP IDMDFNLIPD AAMTIAVAAL FANGTSTLRN IASWRVKETD RIAAMATELR 370 380 390 400 410 420 KVGATVEEGA DYLVVTPPAA LTPNAAIDTY DDHRMAMCFS LVSLGGVPVR INDPKCVGKT 430 FPDYFDRFAA LAKA

References

[1]

"Complete sequence of chromosome 1 of Burkholderia multivorans ATCC 17616." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Tiedje J., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

[2]

"Complete genome sequence of Burkholderia multivorans ATCC 17616." Ohtsubo Y., Yamashita A., Kurokawa K., Takami H., Yuhara S., Nishiyama E., Endo R., Miyazaki R., Ono A., Yano K., Ito M., Sota M., Yuji N., Hattori M., Tsuda M. Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 17616 / 249.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000868 Genomic DNA. Translation: ABX15944.1. AP009385 Genomic DNA. Translation: BAG42926.1.
RefSeq	YP_001580441.1. NC_010084.1. YP_001945462.1. NC_010804.1.
3D structure databases
ProteinModelPortal	A9ADV6.
ModBase	Search...
Protein-protein interaction databases
STRING	A9ADV6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5767467. 6360291.
GenomeReviews	Gene locus BMULJ_00980 in contig AP009385_GR. Gene locus Bmul_2259 in contig CP000868_GR.
KEGG	bmj:BMULJ_00980. bmu:Bmul_2259.
PATRIC	19163682. VBIBurMul203716_1932.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	GADIEWG.
ProtClustDB	PRK02427.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BURM1

Accession

Primary (citable) accession number: A9ADV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program