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A9AB67 (COFG_METM6) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
FO synthase subunit 1

EC=2.5.1.77
Alternative name(s):
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1
Gene names
Name:cofG
Ordered Locus Names:MmarC6_1779
OrganismMethanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]
Taxonomic identifier444158 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus

Protein attributes

Sequence length352 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) By similarity. HAMAP-Rule MF_01611

Catalytic activity

5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3. HAMAP-Rule MF_01611

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Cofactor biosynthesis; coenzyme F0 biosynthesis. HAMAP-Rule MF_01611

Subunit structure

Consists of two subunits, CofG and CofH By similarity. HAMAP-Rule MF_01611

Sequence similarities

Belongs to the radical SAM superfamily. CofG family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 352352FO synthase subunit 1 HAMAP-Rule MF_01611
PRO_1000148236

Sites

Metal binding491Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding531Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
Metal binding561Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9AB67 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 7B4BF967BC7D15D6

FASTA35240,620
        10         20         30         40         50         60 
MITKSEALDF LKFNSINPIL EKLEDINTRN SSKTITFSKN AFIPVCNWCR NVCGYCTFRA 

        70         80         90        100        110        120 
EDFKLLKMEE MKEILIKADV FGCREALFTF GENVDENEKV KEELKKMGYS GILEYLYEIS 

       130        140        150        160        170        180 
AWCLENTNLL PHTNCGILSY DELKYLREVN ASMGLMLENS SERLCSTIAH EKSPGKDPNL 

       190        200        210        220        230        240 
RIEMIENAGK LKIPFTTGIL IGIGETLEER IDSIFEIKRI HEKYGNIQEV IVQNFRSKPK 

       250        260        270        280        290        300 
IPMENYKEPS PVEMFKMIIL SKLILEDISI QVPPNLNRET GQLFLMAGID DWGGVSPLTK 

       310        320        330        340        350 
DFVNPEAPWP DIEELNSFSK ELGFNLKERL PVYEKYIAEE WLDKKILEKI KK 

« Hide

References

[1]"Complete sequence of Methanococcus maripaludis C6."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000867 Genomic DNA. Translation: ABX02590.1.
RefSeqYP_001549822.1. NC_009975.1.

3D structure databases

ProteinModelPortalA9AB67.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING444158.MmarC6_1779.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX02590; ABX02590; MmarC6_1779.
GeneID5737633.
KEGGmmx:MmarC6_1779.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1060.
HOGENOMHOG000108301.
KOK11780.
OMALTTACRY.

Enzyme and pathway databases

BioCycMMAR444158:GHN2-1826-MONOMER.
UniPathwayUPA00072.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01611. FO_synth_sub1.
InterProIPR013785. Aldolase_TIM.
IPR019939. CofG_family.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR03550. F420_cofG. 1 hit.
ProtoNetSearch...

Entry information

Entry nameCOFG_METM6
AccessionPrimary (citable) accession number: A9AB67
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways