A9AB67 (COFG_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
FO synthase subunit 1
EC=2.5.1.77

Alternative name(s):
7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 1

Gene names

Name:	cofG
Ordered Locus Names:	MmarC6_1779

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

cellular organisms › Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus › Methanococcus maripaludis

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino-6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) By similarity. HAMAP-Rule MF_01611
Catalytic activity	5-amino-6-(D-ribitylamino)uracil + 3-(4-hydroxyphenyl)pyruvate + 2 S-adenosyl-L-methionine + H2O = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 2 L-methionine + 2 5'-deoxyadenosine + oxalate + NH3. HAMAP-Rule MF_01611
Cofactor	Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Cofactor biosynthesis; coenzyme F0 biosynthesis. HAMAP-Rule MF_01611
Subunit structure	Consists of two subunits, CofG and CofH By similarity.
Sequence similarities	Belongs to the radical SAM superfamily. CofG family.

Ontologies

Keywords
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	coenzyme biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	FO synthase subunit 1 HAMAP-Rule MF_01611		PRO_1000148236
Sites
	Metal binding	49	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	53	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity
	Metal binding	56	1	Iron-sulfur (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AB67 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 7B4BF967BC7D15D6	FASTA	352	40,620
	10 20 30 40 50 60 MITKSEALDF LKFNSINPIL EKLEDINTRN SSKTITFSKN AFIPVCNWCR NVCGYCTFRA 70 80 90 100 110 120 EDFKLLKMEE MKEILIKADV FGCREALFTF GENVDENEKV KEELKKMGYS GILEYLYEIS 130 140 150 160 170 180 AWCLENTNLL PHTNCGILSY DELKYLREVN ASMGLMLENS SERLCSTIAH EKSPGKDPNL 190 200 210 220 230 240 RIEMIENAGK LKIPFTTGIL IGIGETLEER IDSIFEIKRI HEKYGNIQEV IVQNFRSKPK 250 260 270 280 290 300 IPMENYKEPS PVEMFKMIIL SKLILEDISI QVPPNLNRET GQLFLMAGID DWGGVSPLTK 310 320 330 340 350 DFVNPEAPWP DIEELNSFSK ELGFNLKERL PVYEKYIAEE WLDKKILEKI KK

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX02590.1.
RefSeq	YP_001549822.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9AB67.
ModBase	Search...
Protein-protein interaction databases
STRING	444158.MmarC6_1779.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX02590; ABX02590; MmarC6_1779.
GeneID	5737633.
KEGG	mmx:MmarC6_1779.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1060.
HOGENOM	HOG000108301.
KO	K11780.
OMA	KLKIPFT.
ProtClustDB	PRK06245.
Enzyme and pathway databases
BioCyc	MMAR444158:GHN2-1825-MONOMER.
UniPathway	UPA00072.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01611. FO_synth_sub1.
InterPro	IPR013785. Aldolase_TIM. IPR019939. CofG_family. IPR006638. Elp3/MiaB/NifB. IPR007197. rSAM. [Graphical view]
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR03550. F420_cofG. 1 hit.
ProtoNet	Search...

Entry information

Entry name

COFG_METM6

Accession

Primary (citable) accession number: A9AB67

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program