A9AAX4 (DNLI_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA ligase
EC=6.5.1.1

Alternative name(s):
Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:	lig
Ordered Locus Names:	MmarC6_1685

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	573 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity. HAMAP MF_00407
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Divalent metal cations By similarity. HAMAP MF_00407
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA ligation involved in DNA repair Inferred from electronic annotation. Source: InterPro DNA recombination Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 573	573	DNA ligase HAMAP MF_00407		PRO_0000365252
Sites
	Active site	252	1	N6-AMP-lysine intermediate By similarity
	Binding site	250	1	ATP By similarity
	Binding site	257	1	ATP By similarity
	Binding site	272	1	ATP By similarity
	Binding site	301	1	ATP By similarity
	Binding site	342	1	ATP By similarity
	Binding site	432	1	ATP By similarity
	Binding site	438	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9AAX4 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: E25F2EC289F2571D	FASTA	573	65,774
	10 20 30 40 50 60 MLFIDFCKIL DKIEKTTKRL EKTDYFVELI DFIKNNGKAE NLKQVSQITI GRVFAEFENK 70 80 90 100 110 120 EIGIGPNLLI EAVKTTGISE KDLKAEIKKT GDIGIAVENL SSNIKQVSLF SQPLTLEEIY 130 140 150 160 170 180 STLKKLSEIE GNSSQKKKTR IISNLLILSN PVESRYISRL ILEDMRIGMN IPTILASFSN 190 200 210 220 230 240 YFNVNKEAVE KIYAVTNDIG LIGKKLISGS DIENDSELKL KVFRPIKPML AQLTPSIEDA 250 260 270 280 290 300 IIETKMPQFE TKYDGARVQV HKSNGKVNIY SRRLENITNS VPELVEEINK IDIDNIILEG 310 320 330 340 350 360 ECVAMDLSSG KPRPFQDILR RFRRKYDIDK VAEKIALRIY FFDVLYYEKG LIDTPLKNRR 370 380 390 400 410 420 EILEKLFGTN NWDSELEKIQ KEIFSNKMLF SSFKLNSDDP DLAKEFFNWS LSIGHEGIMI 430 440 450 460 470 480 KNPDAPYTPG SRVKTMYKVK PTLENLDVVV TRAKIGMGKR KDWYGSYEIS VKDDEDNLHV 490 500 510 520 530 540 IGNVGSGLTE DDLERLTTIV NEIKIEDLGE EVILEPKIVL EVTYEEIQTS EKYEMGYALR 550 560 570 FPRVVQIRED KSINDINTLD DVKKIYEIER NRK

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX02497.1.
RefSeq	YP_001549729.1. NC_009975.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9AAX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5737379.
GenomeReviews	Gene locus MmarC6_1685 in contig CP000867_GR.
KEGG	mmx:MmarC6_1685.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG570821.
OMA	GRPRPFQ.
ProtClustDB	CLSK876100.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_1685-MONOMER.
Family and domain databases
HAMAP	MF_00407. DNA_ligase. [Tree]
InterPro	IPR022865. DNA_ligae_ATP-dep_bac/arc. IPR000977. DNA_ligase_ATP-dep. IPR012309. DNA_ligase_ATP-dep_C. IPR012310. DNA_ligase_ATP-dep_cent. IPR016059. DNA_ligase_ATP-dep_CS. IPR012308. DNA_ligase_ATP-dep_N. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. [Graphical view]
Gene3D	G3DSA:1.10.3260.10. DNA_ligase_ATP-dep_N. 1 hit. G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K10747.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit. SSF117018. SSF117018. 1 hit.
TIGRFAMs	TIGR00574. Dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_METM6

Accession

Primary (citable) accession number: A9AAX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 3, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program