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Protein

Thiamine thiazole synthase

Gene

thi4

Organism
Methanococcus maripaludis (strain C6 / ATCC BAA-1332)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.UniRule annotation

Cofactori

Fe2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei40NADUniRule annotation1
Binding sitei67NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei133NAD; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi161Iron; shared with adjacent protomerUniRule annotation1
Metal bindingi176IronUniRule annotation1
Binding sitei179NAD; via carbonyl oxygenUniRule annotation1
Binding sitei226NAD; via amide nitrogenUniRule annotation1
Binding sitei236GlycineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi59 – 60NADUniRule annotation2
Nucleotide bindingi159 – 161NAD; shared with adjacent protomerUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processThiamine biosynthesis
LigandIron, Metal-binding, NAD

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiamine thiazole synthaseUniRule annotation (EC:4.-.-.-UniRule annotation)
Gene namesi
Name:thi4UniRule annotation
Ordered Locus Names:MmarC6_1321
OrganismiMethanococcus maripaludis (strain C6 / ATCC BAA-1332)
Taxonomic identifieri444158 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000791 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001156111 – 261Thiamine thiazole synthaseAdd BLAST261

Interactioni

Subunit structurei

Homooctamer; tetramer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi444158.MmarC6_1321.

Structurei

3D structure databases

ProteinModelPortaliA9A9W1.
SMRiA9A9W1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the THI4 family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG094200OG.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.

Sequencei

Sequence statusi: Complete.

A9A9W1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDGKLRADEV AVTKSILKST FNMWMDIIDV DVVIVGAGPS GLTAAKYLAQ
60 70 80 90 100
KGFKTVVLER HLSFGGGTWG GGMGFPNIVV EKPADDILRE AGIKLDEVDG
110 120 130 140 150
EEELFTADSV EVPAKLGVAA IDAGAKILTG IVVEDLILKE DKIAGVVIQS
160 170 180 190 200
YAIEKAGLHI DPLTISAKYV IDSTGHDASA VHTLARKNKD LGIEVPGEKS
210 220 230 240 250
MWAEKGENSL TRNTREIFPG LYVCGMAANA YHAGYRMGAI FGGMYLSGKK
260
CAEMILEKME K
Length:261
Mass (Da):27,919
Last modified:January 15, 2008 - v1
Checksum:i46B0BB435D0A610C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000867 Genomic DNA. Translation: ABX02134.1.
RefSeqiWP_012194078.1. NC_009975.1.

Genome annotation databases

EnsemblBacteriaiABX02134; ABX02134; MmarC6_1321.
GeneIDi5737733.
KEGGimmx:MmarC6_1321.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000867 Genomic DNA. Translation: ABX02134.1.
RefSeqiWP_012194078.1. NC_009975.1.

3D structure databases

ProteinModelPortaliA9A9W1.
SMRiA9A9W1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444158.MmarC6_1321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX02134; ABX02134; MmarC6_1321.
GeneIDi5737733.
KEGGimmx:MmarC6_1321.

Phylogenomic databases

eggNOGiarCOG00574. Archaea.
COG1635. LUCA.
HOGENOMiHOG000106048.
KOiK03146.
OMAiRMGPVFG.
OrthoDBiPOG094200OG.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.50.50.60. 1 hit.
HAMAPiMF_00304. Thi4. 1 hit.
InterProiView protein in InterPro
IPR023753. FAD/NAD-binding_dom.
IPR002922. Thi4_fam.
IPR022828. Thi4_putative.
SUPFAMiSSF51905. SSF51905. 1 hit.
TIGRFAMsiTIGR00292. TIGR00292. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHI4_METM6
AccessioniPrimary (citable) accession number: A9A9W1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: February 15, 2017
This is version 65 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.