A9A8R5 (DP2L_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase II large subunit
Short name=Pol II
EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MmarC6_0923

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase By similarity. HAMAP MF_00324
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00324 Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates. HAMAP MF_00324
Subunit structure	Heterodimer of a large subunit and a small subunit By similarity.
Sequence similarities	Belongs to the archaeal DNA polymerase II family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	DNA-binding
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	DNA catabolic process Inferred from electronic annotation. Source: InterPro DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: UniProtKB-KW DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW exonuclease activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1131	1131	DNA polymerase II large subunit HAMAP MF_00324		PRO_1000116095

Sequences

	Sequence		Length	Mass (Da)
	A9A8R5 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: D859FF8022B7946F	FASTA	1,131	128,285
	10 20 30 40 50 60 MLHVSASKGM AEYFKNILDN VTELYTLADD CRKSGYDVTD HVEIPLAKDM ADRVEGIVGP 70 80 90 100 110 120 KNVAERIREL VSDLGKEPAA LEIAKEIVEG KFGEFGREVG AEQAVRTALA VITEGIVAAP 130 140 150 160 170 180 LEGIAHVKIK KNNDGSEYLA IYFAGPIRSA GGTAQALAVL VGDYVRKNMG LDKFKPTEDE 190 200 210 220 230 240 VERYGEEVDL YQSEVTTFQY QPKAEEIRVA VRNISVEITG EATDDVEVSG HRDLPRVETN 250 260 270 280 290 300 QIRGGALLAL VEGVLLKAPK ILRHVDKLGI EGWDWLKELK SKKEEVIEEI EEEKDDFNYE 310 320 330 340 350 360 EEEDLSQYED YEVEAVTKFI GEVIAGRPVF SHPSKKGGFR LRYGRSRNTG FATDGFHPAI 370 380 390 400 410 420 MYLVDDFMAV GTQLKTERPG KATCVVPVDS IEGPIVKLND KSVLKIDTVE KAKQYRDDVE 430 440 450 460 470 480 EILFLGDILV NYGDFLENNH TILPSSWCVE WYEKILKSEN LEYTKEFIEN PDQKEAVTYA 490 500 510 520 530 540 KLTKTPLHPK YTYFWHDISK DNINVLRNWL IGGKYNENND SWELTYNLED SEIATSKRYL 550 560 570 580 590 600 ELIGCPHTVT AETVEIFEYY PLLYSLGYDF DKKQDIVEDI GEKLQNTKNN MHFINTIAPF 610 620 630 640 650 660 EIRRNAYIYV GARMGRPEKA ASRKMKPPVN GLFPIGNAGA LVRLINKAVE EGKTDEIEIS 670 680 690 700 710 720 NVKCSCGNVS LYRTCPFCGS SVEPSGPSRI KLPIKEYWYK ALENLKINKA GDVKCIKGMT 730 740 750 760 770 780 SKDKIIEPLE KAILRAKNDI FVFKDGTTRF DCTDVPVTHF RPVEIHVGIE KLKFLGYLKD 790 800 810 820 830 840 IHGNPLENEN QVLELKVQDV IVPESCMDYF FNVSKFIDDL LEKYYKKDRF YNVNKREELV 850 860 870 880 890 900 GHLIIGMAPH TSAGMVGRII GYSYANVGYA HPYFHASKRR NCDGDEDAFF LLLDAFMNFS 910 920 930 940 950 960 KRFLPDKRGG QMDAPLVLTT ILDPKEVDGE VHNMDSMWEY PLEFYEKSLE GIAPKEIKKI 970 980 990 1000 1010 1020 MQTVEDRLDK DSQYEGIGYT HETLKIDEGP LVCAYKTLGS MMEKTSAQLA VAKKIRATDE 1030 1040 1050 1060 1070 1080 RDVAEKVIQS HFVPDLIGNL RAFSRQGVRC KCGAKYRRMP LKGVCRKCGS RLILTVSKGA 1090 1100 1110 1120 1130 VEKYMDVSQT MAEKYNASDY IKQRLEIIRS GIDSLFVNDK RKQVKIEDFF K

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01738.1.
RefSeq	YP_001548970.1. NC_009975.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	A9A8R5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5738641.
GenomeReviews	Gene locus MmarC6_0923 in contig CP000867_GR.
KEGG	mmx:MmarC6_0923.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG497074.
OMA	NCDGDED.
ProtClustDB	PRK04023.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_0923-MONOMER.
Family and domain databases
HAMAP	MF_00324. DNApol_II_L_arch. [Tree]
InterPro	IPR004475. PolC_DP2. IPR016033. PolC_DP2_N. [Graphical view]
KO	K02322.
Pfam	PF03833. PolC_DP2. 1 hit. [Graphical view]
PIRSF	PIRSF016275. PolC_DP2. 1 hit.
TIGRFAMs	TIGR00354. PolC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DP2L_METM6

Accession

Primary (citable) accession number: A9A8R5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program