A9A8B3 (A9A8B3_METM6) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180
Short name=DHBP synthase HAMAP MF_00180
EC=4.1.99.12 HAMAP MF_00180

Gene names

Name:	ribB HAMAP MF_00180
Ordered Locus Names:	MmarC6_0769

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP MF_00180
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180 RuleBase RU003843
Subunit structure	Homodimer By similarity. HAMAP MF_00180
Sequence similarities	Belongs to the DHBP synthase family. HAMAP MF_00180 RuleBase RU003843

Ontologies

Keywords
Biological process	Riboflavin biosynthesis HAMAP MF_00180 RuleBase RU003843
Ligand	Magnesium HAMAP MF_00180 Manganese HAMAP MF_00180 RuleBase RU003843 Metal-binding HAMAP MF_00180 RuleBase RU003843
Molecular function	Lyase HAMAP MF_00180 RuleBase RU003843
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Region	45 – 46	2	Substrate binding By similarity HAMAP MF_00180
	Region	181 – 185	5	Substrate binding By similarity HAMAP MF_00180
Sites
	Metal binding	46	1	Magnesium or manganese 1 By similarity HAMAP MF_00180
	Metal binding	46	1	Magnesium or manganese 2 By similarity HAMAP MF_00180
	Metal binding	184	1	Magnesium or manganese 2 By similarity HAMAP MF_00180
	Binding site	50	1	Substrate By similarity HAMAP MF_00180
	Binding site	205	1	Substrate By similarity HAMAP MF_00180
	Site	167	1	Essential for catalytic activity By similarity HAMAP MF_00180
	Site	205	1	Essential for catalytic activity By similarity HAMAP MF_00180

Sequences

	Sequence		Length	Mass (Da)
	A9A8B3 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 27D79AD99075FCB7	FASTA	247	27,726
	10 20 30 40 50 60 MTTDLKNARN SEVKISGDEK ISNVEKAIDA LKSGKIVLLY DSDDREGETD MVVASEFVTP 70 80 90 100 110 120 EHIREMRKNA GGLICTCLHS DFCDKLGIPF MVDILDIAAE KYPVLKELYP NDIPYDEKST 130 140 150 160 170 180 FALYANHRKT FTGITDNDRA LTVKKITELC SEERFNDFGK EFRCPGHVAF LRATKGLVKN 190 200 210 220 230 240 RQGHTEMTVA LAEMAGLTPI TTICEMMGDN GKAMSKDLVK KYAEENGLVT INGQELIEYY LNEFSKE

References

[1]

"Complete sequence of Methanococcus maripaludis C6." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01586.1.
RefSeq	YP_001548818.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9A8B3.
SMR	A9A8B3. Positions 22-240.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A8B3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5737817.
GenomeReviews	Gene locus MmarC6_0769 in contig CP000867_GR.
KEGG	mmx:MmarC6_0769.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG735778.
OMA	GDMIFAA.
ProtClustDB	CLSK876051.
Family and domain databases
HAMAP	MF_00180. RibB. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
KO	K02858.
Pfam	PF00926. DHBP_synthase. 1 hit. [Graphical view]
SUPFAM	SSF55821. DHBP_synth_RibB-like_a/b_dom. 1 hit.
TIGRFAMs	TIGR00506. RibB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

A9A8B3_METM6

Accession

Primary (citable) accession number: A9A8B3

Entry history

Integrated into UniProtKB/TrEMBL:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	November 16, 2011
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)