A9A874 (G1PDH_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol-1-phosphate dehydrogenase [NAD(P)+]
Short name=G1P dehydrogenase
Short name=G1PDH
EC=1.1.1.261

Alternative name(s):
Enantiomeric glycerophosphate synthase
sn-glycerol-1-phosphate dehydrogenase

Gene names

Name:	egsA
Ordered Locus Names:	MmarC6_0730

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	334 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol-1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea By similarity. HAMAP MF_00497_A
Catalytic activity	sn-glycerol-1-phosphate + NAD(P)+ = glycerone phosphate + NAD(P)H. HAMAP MF_00497_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00497_A
Pathway	Membrane lipid metabolism; glycerophospholipid metabolism. HAMAP MF_00497_A
Subcellular location	Cytoplasm Potential HAMAP MF_00497_A.
Sequence similarities	Belongs to the glycerol-1-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Phospholipid biosynthesis
Cellular component	Cytoplasm
Ligand	Metal-binding NAD NADP Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phospholipid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycerol-1-phosphate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 334	334	Glycerol-1-phosphate dehydrogenase [NAD(P)+] HAMAP MF_00497_A		PRO_0000350654
Regions
	Nucleotide binding	77 – 81	5	NAD By similarity
	Nucleotide binding	99 – 102	4	NAD By similarity
Sites
	Metal binding	147	1	Zinc; catalytic By similarity
	Metal binding	225	1	Zinc; catalytic By similarity
	Metal binding	246	1	Zinc; catalytic By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	108	1	NAD By similarity
	Binding site	147	1	Substrate By similarity
	Binding site	229	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9A874 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 6BE4CD76C69DC1C6	FASTA	334	36,774
	10 20 30 40 50 60 MIVIPRYTII KEKASGRIPE ILDNLNLKNP LVITGKNTKK YNKDFDFIYY DEIETSDLEN 70 80 90 100 110 120 IKNYAKDYDS IIGIGGGRPI DIGKLIAHKS KKPFLSVPTT ASNDGIASPI VSLTQPSYMT 130 140 150 160 170 180 EAPIAIIADI DIIKKSPKKL LSAGMGDIVS NITAVLDWEL GKIEKSEKYS DSSGIFSKTI 190 200 210 220 230 240 AIELMDYVLN SNLEEYPKKL VKALIGSGIS IAIAHSSRPA SGSEHLFSHA LDTMKEKYDI 250 260 270 280 290 300 DTTSLHGEQC GVGTLAIAQI YLEEGKLEVE TFEMLKNSLK AVDAPVTAEQ LGFDEEIIIE 310 320 330 ALSSANTLRK RHTILRNGIS KEKAREILEK SEII

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01547.1.
RefSeq	YP_001548779.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9A874.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A874.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5737801.
GenomeReviews	Gene locus MmarC6_0730 in contig CP000867_GR.
KEGG	mmx:MmarC6_0730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG672951.
OMA	CGVGTIM.
ProtClustDB	CLSK876301.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_0730-MONOMER.
Family and domain databases
HAMAP	MF_00497_A. G1P_dehydrogenase_A. [Tree]
InterPro	IPR023002. G1P_dehydrogenase_arc. IPR016205. Glycerol_DH. [Graphical view]
KO	K00096.
PIRSF	PIRSF000112. Glycerol_dehydrogenase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

G1PDH_METM6

Accession

Primary (citable) accession number: A9A874

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 23, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program