A9A787 (SYP_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proline--tRNA ligase
EC=6.1.1.15

Alternative name(s):
Prolyl-tRNA synthetase
Short name=ProRS

Gene names

Name:	proS
Ordered Locus Names:	MmarC6_0188

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro) By similarity. HAMAP MF_01571
Catalytic activity	ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro). HAMAP MF_01571
Subunit structure	Homodimer By similarity. HAMAP MF_01571
Subcellular location	Cytoplasm By similarity HAMAP MF_01571.
Domain	Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension By similarity. HAMAP MF_01571
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	prolyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW proline-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	Proline--tRNA ligase HAMAP MF_01571		PRO_1000215566

Sequences

	Sequence		Length	Mass (Da)
	A9A787 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 0A6EC9A349250276	FASTA	460	53,323
	10 20 30 40 50 60 MEFSEWYSDI LEKAGIYDLR YPIKGCGVYL PYGFKIRRYS FEILRKLLDE TNHDETLFPM 70 80 90 100 110 120 LIPENLLAKE GEHIKGFEDE VFWVTHGGKT PLEVKLALRP TSETTMYYMM KQWIKVHTDL 130 140 150 160 170 180 PMKLYQVVNT FRYETKHTRP LIRLREIMSF KEAHTAHATK EDCDAQITEA LNLYGEFFDE 190 200 210 220 230 240 ICVPYIISKR PEWDKFPGAD YTMAFDTIYP DGKTMQIGTV HNLGQNFAKT FELEFETPDG 250 260 270 280 290 300 EKDFVYQTCY GISDRAIASL ISVHGDEKGL VIPVDVAPIQ IVLIPLLFKG KEEIVMDKIK 310 320 330 340 350 360 ELNRTLKSEF RVLLDDRDIR PGRKYNDWEI KGVPLRIELG PRDIENGQAL IVRRDTGEKI 370 380 390 400 410 420 TVEYSNILEE VEKIVSMYKE NLKIKADEKI KNFLTVVDFE SDVNALSEKV KAALLENKGI 430 440 450 460 ILIPFDESVY NEEFEELIDA SVLGQTTYEG KDYISVARTY

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01011.1.
RefSeq	YP_001548243.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9A787.
SMR	A9A787. Positions 1-460.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A787.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5737991.
GenomeReviews	Gene locus MmarC6_0188 in contig CP000867_GR.
KEGG	mmx:MmarC6_0188.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG334108.
OMA	KFAEYEL.
ProtClustDB	PRK08661.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_0188-MONOMER.
Family and domain databases
HAMAP	MF_01571. Pro_tRNA_synth_type3. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-dom. IPR006195. aa-tRNA-synth_II. IPR004154. Anticodon-bd. IPR002316. Pro-tRNA-synth_IIa. IPR004499. Pro-tRNA-synth_IIa_arc-type. IPR017449. Pro-tRNA_synth_II. IPR015264. Pro-tRNA_synth_II_arc. IPR016061. Pro-tRNA_synth_II_C. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit. G3DSA:3.30.110.30. Pro-tRNA-synth_II_C_arc/euk. 1 hit.
KO	K01881.
PANTHER	PTHR11451:SF6. ProS_fam_I. 1 hit.
Pfam	PF03129. HGTP_anticodon. 1 hit. PF09181. ProRS-C_2. 1 hit. PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PRINTS	PR01046. TRNASYNTHPRO.
SUPFAM	SSF52954. Anticodon_bd. 1 hit. SSF64586. Pro-tRNA_synth_II_C. 1 hit.
TIGRFAMs	TIGR00408. ProS_fam_I. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYP_METM6

Accession

Primary (citable) accession number: A9A787

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program