A9A6Q2 (SYA_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine--tRNA ligase
EC=6.1.1.7

Alternative name(s):
Alanyl-tRNA synthetase
Short name=AlaRS

Gene names

Name:	alaS
Ordered Locus Names:	MmarC6_0513

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	892 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity. HAMAP MF_00036_A
Catalytic activity	ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala). HAMAP MF_00036_A
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00036_A
Subcellular location	Cytoplasm By similarity HAMAP MF_00036_A.
Domain	Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity. HAMAP MF_00036_A
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding RNA-binding Zinc tRNA-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW alanine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 892	892	Alanine--tRNA ligase HAMAP MF_00036_A		PRO_0000347886
Sites
	Metal binding	596	1	Zinc By similarity
	Metal binding	600	1	Zinc By similarity
	Metal binding	700	1	Zinc By similarity
	Metal binding	704	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9A6Q2 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 574296E8E1E25D63	FASTA	892	101,482
	10 20 30 40 50 60 MEINHDYRVK LFDELSFERK QCTECNQWFW TLDKDRTTCG DSPCDEYSFI GSPITSKKYT 70 80 90 100 110 120 YNEMVKEFTN FFAEKGHSPV KRSPVVAKRW RDDILLTIAS IAVFQPWVTN GLVKPVKNPL 130 140 150 160 170 180 VIAQPCIRLN DIDNVGRTGR HLTCFTMGAH HAFNSKDEYK YWTDKTVEYC FELMQRLGID 190 200 210 220 230 240 GKTITFIESW WEGGGNAGPC YEVITHGVEL ATLVFMQYKK VGNDYEEIPL KIVDTGYGIE 250 260 270 280 290 300 RFAWASQGTP TVYESLFSEI IEKLKEDAGI PEVDEKIMAE SATLAGLMDI ENVGDLRVLR 310 320 330 340 350 360 QKVAEKIGMD VDELDKLISP LEYIYAIADH TRCLSFMFGD GIVPSNVKEG YLARLVLRKT 370 380 390 400 410 420 LRYMEKIGIS MSIKDIIAMQ LENMKEIYPE LSEMKEYVMD VLDAEEKKYI QTVNRGRGIV 430 440 450 460 470 480 ERMAASKSEI TLDDLIELYD SNGLPPEIVK DVVDELNKKG KKTIAITVPD NFYTIVAERH 490 500 510 520 530 540 EEEKPEEVVS TKKELPELEV SETELLFFKH PTQAEFEAKI LKIAEKYVVL DKTLFYAEGG 550 560 570 580 590 600 GQKYDIGQLN DIEVSDVQKK NGIVFHKVSD ISKFKEGDTV KGTLNWENRL KLMRNHTATH 610 620 630 640 650 660 VINAAATRVL GKHIWQTGSN VDTEKGRLDI THYERISREQ VKEIERIANE MVLSKMPVNS 670 680 690 700 710 720 TFMDRNDAEQ KYGFTIYQGG VVPGDTLRII EIEETDVEAC GGTHCSNTSE VGYIKVLKTE 730 740 750 760 770 780 RIQDGVERLE YSTGMGSVSE IAIIEDTLID SAEILGIPND QLPKTVKRFF EEWKEQKKTI 790 800 810 820 830 840 EELQKKVGEL VKYELADKFE KVGDYEVLVE QVSGTPNELM SIADNLAVGN KLIVLMNESD 850 860 870 880 890 YLLCKRGENV ELSMKELIRN IGKGGGKDNL AQGKYSETKE QITEKISQIL NK

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01330.1.
RefSeq	YP_001548562.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9A6Q2.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A6Q2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5738555.
GenomeReviews	Gene locus MmarC6_0513 in contig CP000867_GR.
KEGG	mmx:MmarC6_0513.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392147.
OMA	MFTNSGM.
ProtClustDB	PRK13902.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_0513-MONOMER.
Family and domain databases
HAMAP	MF_00036_A. Ala_tRNA_synth_A. [Tree]
InterPro	IPR002318. Ala-tRNA-synth_IIc. IPR018162. Ala-tRNA-synth_IIc_anticod-bd. IPR018165. Ala-tRNA-synth_IIc_core. IPR018164. Ala-tRNA-synth_IIc_N. IPR022429. Ala-tRNA_synth_arc. IPR018163. Thr/Ala-tRNA-synth_IIc_edit. IPR012947. tRNA_SAD. [Graphical view]
KO	K01872.
PANTHER	PTHR11777:SF6. PTHR11777:SF6. 1 hit.
Pfam	PF01411. tRNA-synt_2c. 1 hit. PF07973. tRNA_SAD. 1 hit. [Graphical view]
PRINTS	PR00980. TRNASYNTHALA.
SMART	SM00863. tRNA_SAD. 1 hit. [Graphical view]
SUPFAM	SSF101353. Ala-tRNA-synth_IIc_anticod-bd. 1 hit. SSF55186. Thr/Ala-tRNA-synth_IIc_edit. 1 hit.
TIGRFAMs	TIGR03683. A-tRNA_syn_arch. 1 hit. TIGR00344. AlaS. 1 hit.
PROSITE	PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYA_METM6

Accession

Primary (citable) accession number: A9A6Q2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program