A9A6N6 (ILVD_METM6) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	MmarC6_0633

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000089395
Sites
	Metal binding	119	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	191	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	A9A6N6 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 83D53E4CFF63245F	FASTA	550	58,653
	10 20 30 40 50 60 MISDNVKKGV IRSPNRALLK ACGYSDEDME KPFIGVVNSF TEVVPGHIHL KTLSDVVKHG 70 80 90 100 110 120 VYANGGTPFE FNTIGICDGI AMGHEGMKYS LPSREIIADA VESMARAHGF DGLVLIPTCD 130 140 150 160 170 180 KIVPGMIMGA LRLNIPFIVV TGGPMLPGEF QGKKYELISL FEGVGEYQVG KITEEELRCI 190 200 210 220 230 240 EDCACPGAGS CAGLYTANSM ACLTEALGLS LPMCATTHAI DAQKVRLAKK SGSKIVNMVK 250 260 270 280 290 300 EDLKPTDILT KEAFENAILV DLALGGSTNT TLHIPAIANE IENKFITLDD FDRLSDEVPH 310 320 330 340 350 360 IASIKPGGNH YMIDLHNAGG IPAVFNVLKE KIRNTKTVDG RSTLEIAESV KYINYNVIRK 370 380 390 400 410 420 VEAPVHETAG LRVLKGNLAP NGCVVKIGAV NPKMHKHEGP AKVYNSEDEA ISAILGGEIV 430 440 450 460 470 480 KGDVVVIRYE GPSGGPGMRE MLSPTSAICG MGLDDSVALI TDGRFSGGSR GPCIGHVSPE 490 500 510 520 530 540 AAAGGVIAAI ENGDIIKIDM IGKEINVDLD ESVIKERLSK LGEFEPKIKK GYLSRYSRLV 550 SSADEGAVLK

References

[1]

"Complete sequence of Methanococcus maripaludis C6." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C6 / ATCC BAA-1332.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01450.1.
RefSeq	YP_001548682.1. NC_009975.1.
3D structure databases
ProteinModelPortal	A9A6N6.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A6N6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5738900.
GenomeReviews	Gene locus MmarC6_0633 in contig CP000867_GR.
KEGG	mmx:MmarC6_0633.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SQDCRWP.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	MMAR444158:MMARC6_0633-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_METM6

Accession

Primary (citable) accession number: A9A6N6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program