Reviewed, UniProtKB/Swiss-Prot A9A6M9 (G3P_METM6)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase
      Short name=GAPDH
    EC=1.2.1.59
Alternative name(s):
    NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase

Gene names

Name:	gap
Ordered Locus Names:	MmarC6_0626

Organism

Methanococcus maripaludis (strain C6 / ATCC BAA-1332) [Complete proteome] [HAMAP]

Taxonomic identifier

444158 [NCBI]

Taxonomic lineage

Archaea › Euryarchaeota › Methanococci › Methanococcales › Methanococcaceae › Methanococcus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(P)+ = 3-phospho-D-glyceroyl phosphate + NAD(P)H. HAMAP MF_00559
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5. HAMAP MF_00559
Subunit structure	Homotetramer By similarity. HAMAP MF_00559
Subcellular location	Cytoplasm By similarity HAMAP MF_00559.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro NADP or NADPH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NAD(P)+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Glyceraldehyde-3-phosphate dehydrogenase HAMAP MF_00559		PRO_1000129246
Regions
	Nucleotide binding	11 – 12	2	NAD By similarity
	Region	140 – 142	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	195 – 196	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	141	1	Nucleophile By similarity
	Binding site	111	1	NAD; via amide nitrogen By similarity
	Binding site	169	1	NAD By similarity
	Binding site	303	1	NAD; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9A6M9-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 15F18EC83EF0F352	FASTA	340	37,329
	10 20 30 40 50 60 MVNVLINGYG SIGKRVADAV AKQDDMKVIG VTKTKPDFEA RMAVEKGYKL FAAIPERKHL 70 80 90 100 110 120 FEEAGIPVEG TLDDIIEDAD IVVDGAPKKI GKANLENVYK KHGVKAIIQG GEKAGDAQDS 130 140 150 160 170 180 FNSLWSYNRC YGKDYIRLVS CNTTGLCRSM YAINSVADIL KARIVLIRRA ADPNDVKTGP 190 200 210 220 230 240 VNAIVPNPVS VPSHHGPDVV SVIPELDGKI MTSAVIVPTT LMHMHSIMVE TSGTNRDEII 250 260 270 280 290 300 DALAKTPRIL TLKASEGFDS TAKIIEYARD LGRSRYDLNE IAVWEESVNV VDNEVYMMQA 310 320 330 340 IHQESDVIPE NVDCIRAMLE MESDNLKSIE KTNKAMGLIK

References

[1]

"Complete sequence of Methanococcus maripaludis C6." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Clum A., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Sieprawska-Lupa M., Whitman W.B., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000867 Genomic DNA. Translation: ABX01443.1.
RefSeq	YP_001548675.1.
3D structure databases
SMR	A9A6M9. Positions 4-338.
ModBase	Search...
Genome annotation databases
GeneID	5738892.
GenomeReviews	Gene locus MmarC6_0626 in contig CP000867_GR.
KEGG	mmx:MmarC6_0626.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG392099.
OMA	VHQESDV.
Family and domain databases
HAMAP	MF_00559. G3P_dehdrog_arch. [Tree]
InterPro	IPR020830. GlycerAld_3-P_DH_AS. IPR020829. GlycerAld_3-P_DH_cat. IPR020831. GlycerAld_3-P_DH_family. IPR020828. GlycerAld_3-P_DH_NAD(P)_bd. IPR006436. Glyceraldehyde-3-P_DH_2_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
SMART	SM00846. Gp_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01546. GAPDH-II_archae. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P_METM6

Accession

Primary (citable) accession number: A9A6M9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)