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Protein

Preflagellin peptidase

Gene

flaK

Organism
Methanococcus maripaludis (strain C6 / ATCC BAA-1332)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Cleaves the N-terminal leader peptide from preflagellins.1 Publication

Catalytic activityi

Cleaves the signal peptide of 3 to 12 amino acids from the N-terminal of preflagellin, usually at Arg-Gly-|- or Lys-Gly-|-, to release flagellin.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei18Essential for catalysisBy similarity1
Sitei79Essential for catalysisBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease

Keywords - Biological processi

Archaeal flagellum biogenesis

Enzyme and pathway databases

BRENDAi3.4.23.52. 3262.

Protein family/group databases

MEROPSiA24.016.

Names & Taxonomyi

Protein namesi
Recommended name:
Preflagellin peptidase (EC:3.4.23.52)
Short name:
PFP
Gene namesi
Name:flaK
Ordered Locus Names:MmarC6_0338
OrganismiMethanococcus maripaludis (strain C6 / ATCC BAA-1332)
Taxonomic identifieri444158 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaMethanococciMethanococcalesMethanococcaceaeMethanococcus
Proteomesi
  • UP000000791 Componenti: Chromosome

Subcellular locationi

  • Cell membrane 1 Publication; Multi-pass membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1CytoplasmicCurated1
Transmembranei2 – 18HelicalCuratedAdd BLAST17
Topological domaini19 – 23ExtracellularCurated5
Transmembranei24 – 46HelicalCuratedAdd BLAST23
Topological domaini47 – 49CytoplasmicCurated3
Transmembranei50 – 72HelicalCuratedAdd BLAST23
Topological domaini73 – 78ExtracellularCurated6
Transmembranei79 – 89HelicalCuratedAdd BLAST11
Topological domaini90 – 110CytoplasmicCuratedAdd BLAST21
Transmembranei111 – 139HelicalCuratedAdd BLAST29
Topological domaini140 – 204ExtracellularCuratedAdd BLAST65
Transmembranei205 – 216HelicalCuratedAdd BLAST12
Topological domaini217 – 230CytoplasmicCuratedAdd BLAST14

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi23E → A: No effect on proteolytic activity. 1 Publication1
Mutagenesisi25E → A: No effect on proteolytic activity. 1 Publication1
Mutagenesisi26D → A: No effect on proteolytic activity. 1 Publication1
Mutagenesisi76G → A: Large reduction in proteolytic activity. 1 Publication1
Mutagenesisi77G → A: No effect on proteolytic activity. 1 Publication1
Mutagenesisi78G → A: No effect on proteolytic activity. 1 Publication1
Mutagenesisi208P → A: Enhanced proteolytic activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004192771 – 230Preflagellin peptidaseAdd BLAST230

Interactioni

Protein-protein interaction databases

STRINGi444158.MmarC6_0338.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S0XX-ray3.60A/B1-230[»]
SMRiA9A677.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiarCOG02298. Archaea.
COG1989. LUCA.
HOGENOMiHOG000254257.
KOiK07991.
OMAiEIEDYIW.

Family and domain databases

InterProiIPR009655. Preflagellin_peptidase_C.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06847. Arc_PepC_II. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9A677-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIEYIIGALG LIIASVQDFR SREIEDYIWI FLAVFGVLFA IYSSITLLDY
60 70 80 90 100
SILINSISGF VICFILGYMM FLSGIGGGDG KMLIGLGALV PKFQMPIYTS
110 120 130 140 150
LGTLLNLNYV PTFPIMVFIN GIFFMVFLPF VILFRNILNG ARPKTGKEFI
160 170 180 190 200
LMFFGEKMKV NVAKEQKRLI MGQNDKINFF PAADDEDFSK YSNNEEIWVT
210 220 230
PQIPLIIPIT LSYLVTPIIG DRILDFLIPF
Length:230
Mass (Da):26,021
Last modified:January 15, 2008 - v1
Checksum:i879C0EE8E214E61A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000867 Genomic DNA. Translation: ABX01159.1.
RefSeqiWP_012193134.1. NC_009975.1.

Genome annotation databases

EnsemblBacteriaiABX01159; ABX01159; MmarC6_0338.
GeneIDi5739084.
KEGGimmx:MmarC6_0338.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000867 Genomic DNA. Translation: ABX01159.1.
RefSeqiWP_012193134.1. NC_009975.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3S0XX-ray3.60A/B1-230[»]
SMRiA9A677.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi444158.MmarC6_0338.

Protein family/group databases

MEROPSiA24.016.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX01159; ABX01159; MmarC6_0338.
GeneIDi5739084.
KEGGimmx:MmarC6_0338.

Phylogenomic databases

eggNOGiarCOG02298. Archaea.
COG1989. LUCA.
HOGENOMiHOG000254257.
KOiK07991.
OMAiEIEDYIW.

Enzyme and pathway databases

BRENDAi3.4.23.52. 3262.

Family and domain databases

InterProiIPR009655. Preflagellin_peptidase_C.
IPR000045. Prepilin_IV_endopep_pep.
[Graphical view]
PfamiPF06847. Arc_PepC_II. 1 hit.
PF01478. Peptidase_A24. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFLAK_METM6
AccessioniPrimary (citable) accession number: A9A677
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.