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A9A5K6 (BIOB_NITMS) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Nmar_0821
OrganismNitrosopumilus maritimus (strain SCM1) [Reference proteome] [HAMAP]
Taxonomic identifier436308 [NCBI]
Taxonomic lineageArchaeaThaumarchaeotaNitrosopumilalesNitrosopumilaceaeNitrosopumilus

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Biotin synthase HAMAP-Rule MF_01694
PRO_0000381503

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1451Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2041Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2741Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A9A5K6 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 81B17EEB1B67D9ED

FASTA32636,271
        10         20         30         40         50         60 
MSTLEFIKEC QEKVFSGNHI TAEDAEKLLN IPEENLKDLA RCANEITRDF NGEKVDVEQL 

        70         80         90        100        110        120 
NNIKKNACSE DCTFCGQSAF FDTGIETYQL PSPEEVVSKA QKAKEEGAES YCLVAAWREP 

       130        140        150        160        170        180 
SRTDFEKVCK IITEINDKVG ISVECSLGFL TQEQAKKLKD LKVKRYNHNL ETAKSKFPEI 

       190        200        210        220        230        240 
CTTHTYEDRL ETLGIARDAG LELCTGGIIG LGETREQRLE LTLELARLYP EEVTINILVP 

       250        260        270        280        290        300 
VPGTPLELQT DLPNSEIVRM FSVIRFLLPE SVIKISGGRE TNLEDSGEEL LQSGANGIIT 

       310        320 
SGYLTMGGNE AQKDHAMIEK IGLKSQ 

« Hide

References

[1]"Complete sequence of Nitrosopumilus maritimus SCM1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Stahl D., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: SCM1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000866 Genomic DNA. Translation: ABX12717.1.
RefSeqYP_001582155.1. NC_010085.1.

3D structure databases

ProteinModelPortalA9A5K6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING436308.Nmar_0821.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX12717; ABX12717; Nmar_0821.
GeneID5773808.
KEGGnmr:Nmar_0821.

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OMADETQALC.

Enzyme and pathway databases

BioCycNMAR436308:GI3J-848-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_NITMS
AccessionPrimary (citable) accession number: A9A5K6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways