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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Nitrosopumilus maritimus (strain SCM1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131PhosphateUniRule annotation
Sitei168 – 1681Important for substrate specificityUniRule annotation
Binding sitei186 – 1861Substrate; via amide nitrogenUniRule annotation
Binding sitei187 – 1871PhosphateUniRule annotation
Sitei221 – 2211Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

BioCyciNMAR436308:GI3J-1436-MONOMER.
UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Gene namesi
Name:mtnPUniRule annotation
Ordered Locus Names:Nmar_1401
OrganismiNitrosopumilus maritimus (strain SCM1)
Taxonomic identifieri436308 [NCBI]
Taxonomic lineageiArchaeaThaumarchaeotaNitrosopumilalesNitrosopumilaceaeNitrosopumilus
ProteomesiUP000000792 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 263263S-methyl-5'-thioadenosine phosphorylasePRO_0000415105Add
BLAST

Interactioni

Subunit structurei

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi436308.Nmar_1401.

Structurei

3D structure databases

ProteinModelPortaliA9A3N5.
SMRiA9A3N5. Positions 3-261.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni55 – 562Phosphate bindingUniRule annotation
Regioni88 – 892Phosphate bindingUniRule annotation
Regioni210 – 2123Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
KOiK00772.
OMAiMTNHTEA.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A9A3N5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKDVEIGIF GGTGIYDSGL LEDAKEVDID TPYGKPSDTI TVGTFKGRKI
60 70 80 90 100
AFLPRHGKKH TIPPHMINFK ANIWAFKELG VTRIIAPSAV GSLKEELAPG
110 120 130 140 150
HFVLPTQFLD FTKSRDGSFS EDGRVIHISV ADPFCPELQS SITEVTDSLD
160 170 180 190 200
MNIHKDCTYV CIEGPRFSTK AESKFYRTTG ADIIGMTLVP ECQLAREAQI
210 220 230 240 250
CYASISTVTD YDVWAEKPVT AKEVLETLSK NVEGTKKILT ELIEKIPKDR
260
SCSCAKALEE AEF
Length:263
Mass (Da):29,043
Last modified:January 14, 2008 - v1
Checksum:iF42286B331B3BFF9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000866 Genomic DNA. Translation: ABX13297.1.
RefSeqiYP_001582735.1. NC_010085.1.

Genome annotation databases

EnsemblBacteriaiABX13297; ABX13297; Nmar_1401.
GeneIDi5773440.
KEGGinmr:Nmar_1401.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000866 Genomic DNA. Translation: ABX13297.1.
RefSeqiYP_001582735.1. NC_010085.1.

3D structure databases

ProteinModelPortaliA9A3N5.
SMRiA9A3N5. Positions 3-261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi436308.Nmar_1401.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX13297; ABX13297; Nmar_1401.
GeneIDi5773440.
KEGGinmr:Nmar_1401.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228986.
KOiK00772.
OMAiMTNHTEA.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.
BioCyciNMAR436308:GI3J-1436-MONOMER.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: SCM1.

Entry informationi

Entry nameiMTAP_NITMS
AccessioniPrimary (citable) accession number: A9A3N5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2012
Last sequence update: January 14, 2008
Last modified: March 31, 2015
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.