A9A271 (A9A271_NITMS) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase HAMAP MF_00012
Short name=DAD HAMAP MF_00012
EC=4.2.1.9 HAMAP MF_00012

Gene names

Name:	ilvD HAMAP MF_00012
Ordered Locus Names:	Nmar_0886

Organism

Nitrosopumilus maritimus (strain SCM1)

Taxonomic identifier

436308 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › marine archaeal group 1 › Nitrosopumilales › Nitrosopumilaceae › Nitrosopumilus

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster By similarity. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family. HAMAP MF_00012

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis HAMAP MF_00012
Ligand	4Fe-4S HAMAP MF_00012 Iron Iron-sulfur Metal-binding
Molecular function	Lyase HAMAP MF_00012 EMBL ABX12782.1
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012
	Metal binding	194	1	Iron-sulfur (4Fe-4S) By similarity HAMAP MF_00012

Sequences

	Sequence		Length	Mass (Da)
	A9A271 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: E533D1EF078DFD10	FASTA	558	59,458
	10 20 30 40 50 60 MEEISSRNVV EGTSRAPQRA MYKAMGLDDS DLSKQMIGVC HTGNEATPCN IHLPRLALKA 70 80 90 100 110 120 KEGVSDTGAT PREFSTIAVS DGIAMGHEGM KSSLISREVI ADSIELMVRA HQYDALVGIA 130 140 150 160 170 180 GCDKSLPGTM MAMARLNVPS VFVYGGTIMP GMLNGEELTV VDVYEAVGAY DAGQLSLEGL 190 200 210 220 230 240 KNIENTACPN EGSCGGMFTA NTMASISEAI GLALPGSASP PAEDDRREKM VYDTGVACAK 250 260 270 280 290 300 ALQMDIRPRE VLTFEAFENA ITMLNSVGGS TNGILHLLAL ANEVGIKLTY DDFERVRKKT 310 320 330 340 350 360 PHIADMKPGG NYVMNSLDKI GGIPFVLKKL LDKGLLNENC ITITGKTIKE NLNAMVMPQT 370 380 390 400 410 420 EQQIIRPMEN PLHNVGTAVI LKGSLAPDGA VIKTAGVEMT KFTGKARVFD REELAFEAVK 430 440 450 460 470 480 RGDIDEGHVV VIRYEGPKGG PGMREMLATT AALVGQGLGK KVAMVTDGRF SGGTRGFMVG 490 500 510 520 530 540 HVAPEAYVGG PIALVKDDDE ITIDTETNII DLHVSSEELE NRRKQWSPPK PNYTSGALAK 550 FATLVGSAAE GAITKPNL

References

[1]

"Complete sequence of Nitrosopumilus maritimus SCM1." Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Stahl D., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000866 Genomic DNA. Translation: ABX12782.1.
RefSeq	YP_001582220.1. NC_010085.1.
3D structure databases
ProteinModelPortal	A9A271.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A271.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5774374.
GenomeReviews	Gene locus Nmar_0886 in contig CP000866_GR.
KEGG	nmr:Nmar_0886.
Phylogenomic databases
HOGENOM	HBG671001.
OMA	ETVTCAQ.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

A9A271_NITMS

Accession

Primary (citable) accession number: A9A271

Entry history

Integrated into UniProtKB/TrEMBL:	January 15, 2008
Last sequence update:	January 15, 2008
Last modified:	December 14, 2011
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)