A9A1F7 (ASPD_NITMS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable L-aspartate dehydrogenase
EC=1.4.1.21

Gene names

Name:	nadX
Ordered Locus Names:	Nmar_1240

Organism

Nitrosopumilus maritimus (strain SCM1)

Taxonomic identifier

436308 [NCBI]

Taxonomic lineage

Archaea › Thaumarchaeota › marine archaeal group 1 › Nitrosopumilales › Nitrosopumilaceae › Nitrosopumilus

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate By similarity. HAMAP MF_01265
Catalytic activity	L-aspartate + H2O + NAD(P)+ = oxaloacetate + NH3 + NAD(P)H. HAMAP MF_01265
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. HAMAP MF_01265
Miscellaneous	The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia By similarity. HAMAP MF_01265
Sequence similarities	Belongs to the L-aspartate dehydrogenase family.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro NADP catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro aspartate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Probable L-aspartate dehydrogenase HAMAP MF_01265		PRO_1000140088
Sites
	Active site	222	1	By similarity
	Binding site	125	1	NAD; via amide nitrogen By similarity
	Binding site	192	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A9A1F7 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 66E9C99CAF4E57D9	FASTA	272	29,185
	10 20 30 40 50 60 MKRIALLGCG SMGTQIALAI DSENFPATLT HVYDESKDAS FSLTQKLKNK PEIVENSHLL 70 80 90 100 110 120 SSQPIDIVVE AASQNAVKDV ALSVIQNKKD LMIMSVGALL DESIYDILSD ACNDFKKTIY 130 140 150 160 170 180 LPSGAIAGLD GLKSVKDELE SISITTTKHP RSLKGAKFFE TSDINLDEIT SSTVVYKGTA 190 200 210 220 230 240 KEAVTLFPAN INVAALLSLT GIGSEKTSVT IVADPNTDKN THHIEASGKF GTMTFTIENV 250 260 270 PDSNNPKTSR LAILSAIETL KKYCSDDIQI GT

References

[1]

"Complete sequence of Nitrosopumilus maritimus SCM1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Stahl D., Richardson P. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: SCM1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000866 Genomic DNA. Translation: ABX13136.1.
RefSeq	YP_001582574.1. NC_010085.1.
3D structure databases
ProteinModelPortal	A9A1F7.
ModBase	Search...
Protein-protein interaction databases
STRING	A9A1F7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5773947.
GenomeReviews	Gene locus Nmar_1240 in contig CP000866_GR.
KEGG	nmr:Nmar_1240.
Phylogenomic databases
HOGENOM	HBG649642.
OMA	ECAGHSA.
Family and domain databases
HAMAP	MF_01265. NadX. [Tree]
InterPro	IPR005106. Asp/hSer_DH_NAD-bd. IPR002811. Asp_DH. IPR011182. Asp_DH_NAD_syn. IPR020626. Asp_DH_NAD_syn_prok. IPR022487. Asp_DH_NAD_synth_arc. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K06989.
Pfam	PF01958. DUF108. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF005227. Asp_dh_NAD_syn. 1 hit.
TIGRFAMs	TIGR03855. NAD_NadX. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASPD_NITMS

Accession

Primary (citable) accession number: A9A1F7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 14, 2009
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program