ID   FTHS_DESOH              Reviewed;         587 AA.
AC   A8ZZJ0;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   27-MAR-2024, entry version 80.
DE   RecName: Full=Formate--tetrahydrofolate ligase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            EC=6.3.4.3 {ECO:0000255|HAMAP-Rule:MF_01543};
DE   AltName: Full=Formyltetrahydrofolate synthetase {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FHS {ECO:0000255|HAMAP-Rule:MF_01543};
DE            Short=FTHFS {ECO:0000255|HAMAP-Rule:MF_01543};
GN   Name=fhs {ECO:0000255|HAMAP-Rule:MF_01543};
GN   OrderedLocusNames=Dole_3059;
OS   Desulfosudis oleivorans (strain DSM 6200 / JCM 39069 / Hxd3) (Desulfococcus
OS   oleovorans).
OC   Bacteria; Thermodesulfobacteriota; Desulfobacteria; Desulfobacterales;
OC   Desulfosudaceae; Desulfosudis.
OX   NCBI_TaxID=96561;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 6200 / JCM 39069 / Hxd3;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA   Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C.,
RA   Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Wawrik B.,
RA   Richardson P.;
RT   "Complete sequence of Desulfococcus oleovorans Hxd3.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6R)-10-
CC         formyltetrahydrofolate + ADP + phosphate; Xref=Rhea:RHEA:20221,
CC         ChEBI:CHEBI:15740, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474,
CC         ChEBI:CHEBI:57453, ChEBI:CHEBI:195366, ChEBI:CHEBI:456216;
CC         EC=6.3.4.3; Evidence={ECO:0000255|HAMAP-Rule:MF_01543};
CC   -!- PATHWAY: One-carbon metabolism; tetrahydrofolate interconversion.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
CC   -!- SIMILARITY: Belongs to the formate--tetrahydrofolate ligase family.
CC       {ECO:0000255|HAMAP-Rule:MF_01543}.
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DR   EMBL; CP000859; ABW68862.1; -; Genomic_DNA.
DR   RefSeq; WP_012176473.1; NC_009943.1.
DR   AlphaFoldDB; A8ZZJ0; -.
DR   SMR; A8ZZJ0; -.
DR   STRING; 96561.Dole_3059; -.
DR   KEGG; dol:Dole_3059; -.
DR   eggNOG; COG2759; Bacteria.
DR   HOGENOM; CLU_003601_3_3_7; -.
DR   OrthoDB; 9761733at2; -.
DR   UniPathway; UPA00193; -.
DR   Proteomes; UP000008561; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0035999; P:tetrahydrofolate interconversion; IEA:UniProtKB-UniPathway.
DR   CDD; cd00477; FTHFS; 1.
DR   Gene3D; 3.30.1510.10; Domain 2, N(10)-formyltetrahydrofolate synthetase; 1.
DR   Gene3D; 3.10.410.10; Formyltetrahydrofolate synthetase, domain 3; 1.
DR   Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR   HAMAP; MF_01543; FTHFS; 1.
DR   InterPro; IPR000559; Formate_THF_ligase.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   Pfam; PF01268; FTHFS; 1.
DR   SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; One-carbon metabolism;
KW   Reference proteome.
FT   CHAIN           1..587
FT                   /note="Formate--tetrahydrofolate ligase"
FT                   /id="PRO_1000146681"
FT   BINDING         73..80
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01543"
SQ   SEQUENCE   587 AA;  63724 MW;  3D1026866134F143 CRC64;
     MAYDATKMAD WQISEEAEKN MPMPEEWCEK LGLEKEEMLA MGRLSKLDFL KIIKRLEAKP
     DGKYIEVTAI TPTPLGEGKS TTSLGLMEGL GARGKSVGGA LRQPSGGPTM NVKGTAAGGG
     NSLLIPMTEF SLGLTGDIND IMNAHNLGMV AMTARMQHER NYNDEQLQRL TGMRRLDIDP
     TRVEMGWIID FCAQALRNIV IGLGGRTDGY TMQSKFGIAV GSELMAILAV ATDLADLKER
     INNITVAFDK SGKPVTCRDL EVGNAMAAFM RNTINPTLMS TAEYQPCLVH AGPFANIAVG
     QSSIIADRVG LKLWDYHVTE SGFAADIGFE KFWNVKCRFS GLKPHVSVLT ATIRALKMHG
     GGPKVVAGKA LDDAYTKENL ALVEKGVENM VHMIGVIRKS GINPVVCVNR FYTDTDAEVA
     IVKKAAEAAG ARCAESKHWE KGGEGALEFA DAVIDACEEG NDFDFLYPLE MKLRDRVDKI
     AREVYGADGV DWSPEATAKA EMLENDPKYA DFATMMVKTH LSLTHDPVKK GVPKGWRLPI
     RDVLIYSGAK FLCPCAGTIS LMPGTGSNPA FRRIDVDPAT GKVSGLF
//